Figure 1. Glycan structures. The figure represents the glycan transferred to proteins in wild-type S. pombe cells (Glc3Man9GlcNAc2). Lettering corresponds to the order of residue addition in the synthesis of the dolichol-P-P derivative. Numbers between monosaccharides correspond to the carbon atoms involved in the respective linkages. M8A lacks residues g and l-n, M8B residues j and l-n, and M8C residues k-n. The Man7GlcNAc2 isomer formed in S. pombe lacks residues i and k-n. The glycan transferred in MadIA214cells (Man5GlcNAc2) lacks residues h-n.