![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on March 1, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on June 11, 2001
Revised on October 16, 2001
Accepted on December 12, 2001
1 School of Biochemistry and Genetics, The Medical School, University of Newcastle, Newcastle-upon-Tyne NE2 4HH, UK
2 CRC Cell Regulation Group, Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Rd., Manchester M20 4BX, UK
3 Division of Yeast Genetics, National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK
* Corresponding author. E-mail address: mtoone{at}PICR.man.ac.uk.
The signaling pathways that sense adverse stimuli and communicate with the nucleus to initiate appropriate changes in gene expression are central to the cellular stress response. Here, we have characterized the role of the Sty1 (Spc1) stress-activated MAPK pathway, and the Pap1 and Atf1 transcription factors, in regulating the response to H2O2 in the fission yeast, Schizosaccharomyces pombe. We find that H2O2 activates the Sty1 pathway in a dose-dependent manner via at least two sensing mechanisms. At relatively low levels of H2O2, a two component-signaling pathway, which feeds into either of the two stress-activated MAPKKKs, Wak1 or Win1, regulates Sty1 phosphorylation. In contrast, at high levels of H2O2, Sty1 activation is controlled predominantly by a two-component independent mechanism and requires the function of both Wak1 and Win1. Individual transcription factors were also found to function within a limited range of H2O2 concentrations. Pap1 activates target genes primarily in response to low levels of H2O2 while Atf1 primarily controls the transcriptional response to high concentrations of H2O2. Our results demonstrate that S. pombe uses a combination of stress responsive regulatory proteins to gauge and effect the appropriate transcriptional response to increasing concentrations of H2O2.
This article has been cited by other articles:
|
|
 |
|
  T. Udagawa, N. Nemoto, C. R. M. Wilkinson, J. Narashimhan, L. Jiang, S. Watt, A. Zook, N. Jones, R. C. Wek, J. Bahler, et al. Int6/eIF3e Promotes General Translation and Atf1 Abundance to Modulate Sty1 MAPK-dependent Stress Response in Fission Yeast J. Biol. Chem., August 8, 2008; 283(32): 22063 - 22075. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gao, M. K. Davidson, and W. P. Wahls Distinct regions of ATF/CREB proteins Atf1 and Pcr1 control recombination hotspot ade6-M26 and the osmotic stress response Nucleic Acids Res., May 1, 2008; 36(9): 2838 - 2851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Reiter, S. Watt, K. Dawson, C. L. Lawrence, J. Bahler, N. Jones, and C. R. M. Wilkinson Fission Yeast MAP Kinase Sty1 Is Recruited to Stress-induced Genes J. Biol. Chem., April 11, 2008; 283(15): 9945 - 9956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Asp, D. Nilsson, and P. Sunnerhagen Fission Yeast Mitogen-Activated Protein Kinase Sty1 Interacts with Translation Factors Eukaryot. Cell, February 1, 2008; 7(2): 328 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Chen, C. R.M. Wilkinson, S. Watt, C. J. Penkett, W. M. Toone, N. Jones, and J. Bahler Multiple Pathways Differentially Regulate Global Oxidative Stress Responses in Fission Yeast Mol. Biol. Cell, January 1, 2008; 19(1): 308 - 317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Cheetham, D. A. Smith, A. da Silva Dantas, K. S. Doris, M. J. Patterson, C. R. Bruce, and J. Quinn A Single MAPKKK Regulates the Hog1 MAPK Pathway in the Pathogenic Fungus Candida albicans Mol. Biol. Cell, November 1, 2007; 18(11): 4603 - 4614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jara, A. P. Vivancos, I. A. Calvo, A. Moldon, M. Sanso, and E. Hidalgo The Peroxiredoxin Tpx1 Is Essential as a H2O2 Scavenger during Aerobic Growth in Fission Yeast Mol. Biol. Cell, June 1, 2007; 18(6): 2288 - 2295. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Soto, A. Nunez, M. Madrid, J. Vicente, M. Gacto, and J. Cansado Transduction of centrifugation-induced gravity forces through mitogen-activated protein kinase pathways in the fission yeast Schizosaccharomyces pombe Microbiology, May 1, 2007; 153(5): 1519 - 1529. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Lawrence, H. Maekawa, J. L. Worthington, W. Reiter, C. R. M. Wilkinson, and N. Jones Regulation of Schizosaccharomyces pombe Atf1 Protein Levels by Sty1-mediated Phosphorylation and Heterodimerization with Pcr1 J. Biol. Chem., February 23, 2007; 282(8): 5160 - 5170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Rodriguez-Gabriel, S. Watt, J. Bahler, and P. Russell Upf1, an RNA Helicase Required for Nonsense-Mediated mRNA Decay, Modulates the Transcriptional Response to Oxidative Stress in Fission Yeast. Mol. Cell. Biol., September 1, 2006; 26(17): 6347 - 6356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takatsume, S. Izawa, and Y. Inoue Methylglyoxal as a Signal Initiator for Activation of the Stress-activated Protein Kinase Cascade in the Fission Yeast Schizosaccharomyces pombe J. Biol. Chem., April 7, 2006; 281(14): 9086 - 9092. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Martin, M. A. Rodriguez-Gabriel, W. H. McDonald, S. Watt, J. R. Yates III, J. Bahler, and P. Russell Cip1 and Cip2 Are Novel RNA-Recognition-Motif Proteins That Counteract Csx1 Function during Oxidative Stress Mol. Biol. Cell, March 1, 2006; 17(3): 1176 - 1183. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Enjalbert, D. A. Smith, M. J. Cornell, I. Alam, S. Nicholls, A. J.P. Brown, and J. Quinn Role of the Hog1 Stress-activated Protein Kinase in the Global Transcriptional Response to Stress in the Fungal Pathogen Candida albicans Mol. Biol. Cell, February 1, 2006; 17(2): 1018 - 1032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Madrid, T. Soto, H. K. Khong, A. Franco, J. Vicente, P. Perez, M. Gacto, and J. Cansado Stress-induced Response, Localization, and Regulation of the Pmk1 Cell Integrity Pathway in Schizosaccharomyces pombe J. Biol. Chem., January 27, 2006; 281(4): 2033 - 2043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Mutoh, M. Kawabata, and S. Kitajima Effects of Four Oxidants, Menadione, 1-Chloro-2,4-Dinitrobenzene, Hydrogen Peroxide and Cumene Hydroperoxide, on Fission Yeast Schizosaccharmoyces pombe J. Biochem., December 1, 2005; 138(6): 797 - 804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zuin, A. P. Vivancos, M. Sanso, Y. Takatsume, J. Ayte, Y. Inoue, and E. Hidalgo The Glycolytic Metabolite Methylglyoxal Activates Pap1 and Sty1 Stress Responses in Schizosaccharomyces pombe J. Biol. Chem., November 4, 2005; 280(44): 36708 - 36713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Dunand-Sauthier, C. A. Walker, J. Narasimhan, A. K. Pearce, R. C. Wek, and T. C. Humphrey Stress-Activated Protein Kinase Pathway Functions To Support Protein Synthesis and Translational Adaptation in Response to Environmental Stress in Fission Yeast Eukaryot. Cell, November 1, 2005; 4(11): 1785 - 1793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Rodriguez-Gabriel and P. Russell Distinct Signaling Pathways Respond to Arsenite and Reactive Oxygen Species in Schizosaccharomyces pombe Eukaryot. Cell, August 1, 2005; 4(8): 1396 - 1402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Vivancos, E. A. Castillo, B. Biteau, C. Nicot, J. Ayte, M. B. Toledano, and E. Hidalgo A cysteine-sulfinic acid in peroxiredoxin regulates H2O2-sensing by the antioxidant Pap1 pathway PNAS, June 21, 2005; 102(25): 8875 - 8880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Bozonet, V. J. Findlay, A. M. Day, J. Cameron, E. A. Veal, and B. A. Morgan Oxidation of a Eukaryotic 2-Cys Peroxiredoxin Is a Molecular Switch Controlling the Transcriptional Response to Increasing Levels of Hydrogen Peroxide J. Biol. Chem., June 17, 2005; 280(24): 23319 - 23327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Davidson, H. K. Shandilya, K. Hirota, K. Ohta, and W. P. Wahls Atf1-Pcr1-M26 Complex Links Stress-activated MAPK and cAMP-dependent Protein Kinase Pathways via Chromatin Remodeling of cgs2+ J. Biol. Chem., December 3, 2004; 279(49): 50857 - 50863. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Nicholls, M. Straffon, B. Enjalbert, A. Nantel, S. Macaskill, M. Whiteway, and A. J. P. Brown Msn2- and Msn4-Like Transcription Factors Play No Obvious Roles in the Stress Responses of the Fungal Pathogen Candida albicans Eukaryot. Cell, October 1, 2004; 3(5): 1111 - 1123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Madrid, T. Soto, A. Franco, V. Paredes, J. Vicente, E. Hidalgo, M. Gacto, and J. Cansado A Cooperative Role for Atf1 and Pap1 in the Detoxification of the Oxidative Stress Induced by Glucose Deprivation in Schizosaccharomyces pombe J. Biol. Chem., October 1, 2004; 279(40): 41594 - 41602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Smith, S. Nicholls, B. A. Morgan, A. J.P. Brown, and J. Quinn A Conserved Stress-activated Protein Kinase Regulates a Core Stress Response in the Human Pathogen Candida albicans Mol. Biol. Cell, September 1, 2004; 15(9): 4179 - 4190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Watson, J. Mata, J. Bahler, A. Carr, and T. Humphrey Global Gene Expression Responses of Fission Yeast to Ionizing Radiation Mol. Biol. Cell, February 1, 2004; 15(2): 851 - 860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Castillo, A. P. Vivancos, N. Jones, J. Ayte, and E. Hidalgo Schizosaccharomyces pombe Cells Lacking the Ran-binding Protein Hba1 Show a Multidrug Resistance Phenotype Due to Constitutive Nuclear Accumulation of Pap1 J. Biol. Chem., October 17, 2003; 278(42): 40565 - 40572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tatebe and K. Shiozaki Identification of Cdc37 as a Novel Regulator of the Stress-Responsive Mitogen-Activated Protein Kinase Mol. Cell. Biol., August 1, 2003; 23(15): 5132 - 5142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Paredes, A. Franco, T. Soto, J. Vicente-Soler, M. Gacto, and J. Cansado Different roles for the stress-activated protein kinase pathway in the regulation of trehalose metabolism in Schizosaccharomyces pombe Microbiology, July 1, 2003; 149(7): 1745 - 1752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. S. Moye-Rowley Regulation of the Transcriptional Response to Oxidative Stress in Fungi: Similarities and Differences Eukaryot. Cell, June 1, 2003; 2(3): 381 - 389. [Full Text] [PDF]
|
|
|
|
|
|
R. Alonso-Monge, F. Navarro-Garcia, E. Roman, A. I. Negredo, B. Eisman, C. Nombela, and J. Pla The Hog1 Mitogen-Activated Protein Kinase Is Essential in the Oxidative Stress Response and Chlamydospore Formation in Candidaalbicans Eukaryot. Cell, April 1, 2003; 2(2): 351 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Chen, W. M. Toone, J. Mata, R. Lyne, G. Burns, K. Kivinen, A. Brazma, N. Jones, and J. Bahler Global Transcriptional Responses of Fission Yeast to Environmental Stress Mol. Biol. Cell, January 1, 2003; 14(1): 214 - 229. [Abstract] [Full Text]
|
|
|
|
|
|
E. A. Veal, W. M. Toone, N. Jones, and B. A. Morgan Distinct Roles for Glutathione S-Transferases in the Oxidative Stress Response in Schizosaccharomyces pombe J. Biol. Chem., September 13, 2002; 277(38): 35523 - 35531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Smith, W. M. Toone, D. Chen, J. Bahler, N. Jones, B. A. Morgan, and J. Quinn The Srk1 Protein Kinase Is a Target for the Sty1 Stress-activated MAPK in Fission Yeast J. Biol. Chem., August 30, 2002; 277(36): 33411 - 33421. [Abstract] [Full Text] [PDF]
|
|
