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A more recent version of this article appeared on February 1, 2002
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Submitted on June 18, 2001
Revised on October 31, 2001
Accepted on November 14, 2001
1 Department of Pharmacology and Cancer Biology, Duke University Medical Center, C370 LSRC, Research Drive, Durham, NC 27710
* Corresponding author. E-mail address: kornb001{at}mc.duke.edu.
In response to many different apoptotic stimuli, cytochrome c is released from the intermembrane space of the mitochondria into the cytoplasm, where it serves as a co-factor in the activation of pro-caspase 9. Inhibition of this process can occur either by preventing cytochrome c release or by blocking caspase activation or activity. Experiments involving in vitro reconstitution of apoptosis in cell-free extracts of Xenopus laevis eggs have suggested that extracts arrested in interphase are susceptible to an endogenous apoptotic program leading to caspase activation, while extracts arrested in meiotic metaphase are not. We report here that Mos/MEK/MAPK pathways active in M phase-arrested eggs are responsible for rendering them refractory to apoptosis. Interestingly, M phase arrested extracts are competent to release cytochrome c, yet still do not activate caspases. Concomitantly, we have also demonstrated that recombinant Mos, MEK and ERK are sufficient to block cytochrome-c-dependent caspase activation in purified Xenopus cytosol, which lacks both transcription and translation. These data indicate that the MAP kinase pathway can target and inhibit post-cytochrome c release apoptotic events in the absence of new mRNA/protein synthesis, and that this biochemical pathway is responsible for the apoptotic inhibition observed in meiotic Xenopus laevis egg extracts.
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