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MBC in Press, published online ahead of print December 7, 2001
Mol. Biol. Cell 10.1091/mbc.01-06-0318

A more recent version of this article appeared on January 1, 2002
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Submitted on June 18, 2001
Revised on September 19, 2001
Accepted on October 10, 2001

Cytoplasmic localization and evolutionary conservation of MEI-218, a protein required for meiotic crossing over in Drosophila

Elizabeth A. Manheim1, Janet K. Jang1, Danielle Dominic1, and Kim S. McKim1*

1 Waksman Institute, Rutgers University, Piscataway, New Jersey

* Corresponding author. E-mail address: mckim{at}rci.rutgers.edu.

During Drosophila oogenesis, the oocyte is formed within a 16-cell cyst immediately following four incomplete cell divisions. One of the primary events in oocyte development is meiotic recombination. Here, we report on the intracellular localization of the mei-218 protein that is specifically required for meiotic crossing over. In order to understand the role of mei-218 in meiosis and to study the regulation of genes required for meiotic recombination, we characterized the expression pattern of its RNA and protein. Further, we cloned and sequenced mei-218 from two other Drosophila species. The mei-218 RNA and protein have a similar expression pattern, appearing first in early meiotic prophase and then rapidly disappearing as prophase is completed. This pattern corresponds to a specific appearance of the mei-218 gene product in the region of the ovary where meiotic prophase occurs. Although mei-218 is required for 95 percent of all crossovers, the protein is found exclusively in the cytoplasm. Based on these results, we suggest that mei-218 does not have a direct role in recombination but rather regulates other factors required for the production of crossovers. We propose that mei-218 is a molecular link between oocyte differentiation and meiosis.




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