Molecular Biology of the Cell Sign up for new MBC in Press e-TOCs!

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print February 22, 2002
Mol. Biol. Cell 10.1091/mbc.01-08-0422

A more recent version of this article appeared on April 1, 2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
01-08-0422v1
13/4/1329    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Walker, D. S.
Right arrow Articles by Baylis, H. A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Walker, D. S.
Right arrow Articles by Baylis, H. A.

Submitted on August 24, 2001
Revised on January 7, 2002
Accepted on January 18, 2002

Regulated Disruption of Inositol 1,4,5-trisphosphate (IP3) Signaling in Caenorhabditis elegans Reveals New Functions in Feeding and Embryogenesis

Denise S. Walker1, Nicholas J.D. Gower2, Sung Ly1, Gemma L. Bradley3, and Howard A. Baylis1*

1 Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, UK
2 Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, UK, and Laboratory of Receptor Signaling, The Babraham Institute, Cambridge, CB2 4AT, UK
3 Department of Zoology, University of Cambridge, Downing Street, Cambridge, CB2 3EJ, UK (present address: University College London, Department of Biochemistry and Medical Biology, Darwin Building, Gower Street, London, WC1E 6BT, UK)

* Corresponding author. E-mail address: hab{at}mole.bio.cam.ac.uk.

Inositol 1,4,5 trisphosphate (IP3) is an important second messenger in animal cells and is central to a wide range of cellular responses. The major intracellular activity of IP3 is to regulate release of Ca2+ from intracellular stores through IP3 receptors (IP3Rs). We describe a system for the transient disruption of IP3 signaling in the model organism Caenorhabditis elegans. The IP3 binding domain of the C. elegans IP3R, ITR-1, was expressed from heat-shock induced promoters in live animals. This results in a dominant-negative effect caused by the over-expressed IP3 binding domain acting as an IP3 "sponge". Disruption of IP3 signaling resulted in disrupted defecation, a phenotype predicted by previous genetic studies. This approach also identified two new IP3-mediated processes. Firstly, the up-regulation of pharyngeal pumping in response to food is dependant on IP3 signaling. RNAi studies and analysis of itr-1 mutants show that this process is also IP3R dependant. Secondly, the tissue specific expression of the dominant-negative construct enabled us to circumvent the sterility associated with loss of IP3 signaling through the IP3R and thus determine that IP3-mediated signaling is required for multiple steps in embryogenesis, including cytokinesis and gastrulation.




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
S. M. Tokuoka, A. Saiardi, and S. J. Nurrish
The Mood Stabilizer Valproate Inhibits both Inositol- and Diacylglycerol-signaling Pathways in Caenorhabditis elegans
Mol. Biol. Cell, May 1, 2008; 19(5): 2241 - 2250.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
X. Yan, J. Xing, C. Lorin-Nebel, A. Y. Estevez, K. Nehrke, T. Lamitina, and K. Strange
Function of a STIM1 Homologue in C. elegans: Evidence that Store-operated Ca2+ Entry Is Not Essential for Oscillatory Ca2+ Signaling and ER Ca2+ Homeostasis
J. Gen. Physiol., October 1, 2006; 128(4): 443 - 459.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
W. Zhong and P. W. Sternberg
Genome-wide prediction of C. elegans genetic interactions.
Science, March 10, 2006; 311(5766): 1481 - 1484.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
M. Whitaker
Calcium at Fertilization and in Early Development
Physiol Rev, January 1, 2006; 86(1): 25 - 88.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
H. Parry, A. McDougall, and M. Whitaker
Microdomains bounded by endoplasmic reticulum segregate cell cycle calcium transients in syncytial Drosophila embryos
J. Cell Biol., October 10, 2005; 171(1): 47 - 59.
[Abstract] [Full Text] [PDF]

Home page
 JGPHome page
M. V. Espelt, A. Y. Estevez, X. Yin, and K. Strange
Oscillatory Ca2+ Signaling in the Isolated Caenorhabditis elegans Intestine: Role of the Inositol-1,4,5-trisphosphate Receptor and Phospholipases C {beta} and {gamma}
J. Gen. Physiol., September 26, 2005; 126(4): 379 - 392.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
N. J. D. Gower, D. S. Walker, and H. A. Baylis
Inositol 1,4,5-Trisphosphate Signaling Regulates Mating Behavior in Caenorhabditis elegans Males
Mol. Biol. Cell, September 1, 2005; 16(9): 3978 - 3986.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
X. Yin, N. J.D. Gower, H. A. Baylis, and K. Strange
Inositol 1,4,5-Trisphosphate Signaling Regulates Rhythmic Contractile Activity of Myoepithelial Sheath Cells in Caenorhabditis elegans
Mol. Biol. Cell, August 1, 2004; 15(8): 3938 - 3949.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. A. Steger and L. Avery
The GAR-3 Muscarinic Receptor Cooperates With Calcium Signals to Regulate Muscle Contraction in the Caenorhabditis elegans Pharynx
Genetics, June 1, 2004; 167(2): 633 - 643.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
J. A. T. DOW and S. A. DAVIES
Integrative Physiology and Functional Genomics of Epithelial Function in a Genetic Model Organism
Physiol Rev, July 1, 2003; 83(3): 687 - 729.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2002 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.