Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print February 22, 2002
Mol. Biol. Cell 10.1091/mbc.01-11-0531

A more recent version of this article appeared on April 1, 2002
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
01-11-0531v1
13/4/1417    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pan, J.
Right arrow Articles by Snell, W. J.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Pan, J.
Right arrow Articles by Snell, W. J.

Submitted on November 1, 2001
Revised on January 3, 2002
Accepted on January 18, 2002

Kinesin-II is required for flagellar sensory transduction during fertilization in

Junmin Pan1 and William J. Snell1*

1 Department of Cell Biology, University of Texas Southwestern Medical School, 5323 Harry Hines Blvd., Dallas, TX 75390-9039

* Corresponding author. E-mail address: william.snell{at}utsouthwestern.edu.

The assembly and maintenance of eucaryotic flagella and cilia depend on the microtubule motor, kinesin-II. This plus end-directed motor carries intraflagellar transport (IFT) particles from the base to the tip of the organelle, where structural components of the axoneme are assembled. Here we test the idea that kinesin-II also is essential for signal transduction. When mating type plus (mt+) and mating type minus (mt-) gametes of the unicellular green alga Chlamydomonas are mixed together, binding interactions between mt+ and mt- flagellar adhesion molecules, the agglutinins, initiate a signaling pathway that leads to increases in intracellular cAMP, gamete activation, and zygote formation. A critical question in Chlamydomonas fertilization has been how agglutinin interactions are coupled to increases in intracellular cAMP. Recently, Piperno et al. (1996. J. Cell Biol.133:371-9), reported that fla10 gametes with a temperature-sensitive defect in FLA10 kinesin-II did not form zygotes at the restrictive temperature (32 °C). We find that while the rates and extents of flagellar adhesion in fla10 gametes at 32 °C are indistinguishable from wild type gametes, the cells do not undergo gamete activation. On the other hand, 32 °C fla10 gametes regulated agglutinin location and underwent gamete fusion when the cells were incubated in dibutyryl cAMP, indicating that their capacity to respond to the cAMP signal was intact. We show that the cellular defect in the 32 °C, fla10 gametes is a failure to undergo increases in cAMP during flagella adhesion. Thus, in addition to being essential for assembly and maintenance of the structural components of flagella, kinesin-II/IFT plays a role in sensory transduction in these organelles.




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
J. C. Hoeng, S. C. Dawson, S. A. House, M. S. Sagolla, J. K. Pham, J. J. Mancuso, J. Lowe, and W. Z. Cande
High-Resolution Crystal Structure and In Vivo Function of a Kinesin-2 Homologue in Giardia intestinalis
Mol. Biol. Cell, July 1, 2008; 19(7): 3124 - 3137.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
K. Huang, D. R. Diener, A. Mitchell, G. J. Pazour, G. B. Witman, and J. L. Rosenbaum
Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella
J. Cell Biol., November 5, 2007; 179(3): 501 - 514.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
Y. Hou, G. J. Pazour, and G. B. Witman
A Dynein Light Intermediate Chain, D1bLIC, Is Required for Retrograde Intraflagellar Transport
Mol. Biol. Cell, October 1, 2004; 15(10): 4382 - 4394.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
K. Huang, T. Kunkel, and C. F. Beck
Localization of the Blue-Light Receptor Phototropin to the Flagella of the Green Alga Chlamydomonas reinhardtii
Mol. Biol. Cell, August 1, 2004; 15(8): 3605 - 3614.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Wang and W. J. Snell
Flagellar Adhesion between Mating Type Plus and Mating Type Minus Gametes Activates a Flagellar Protein-tyrosine Kinase during Fertilization in Chlamydomonas
J. Biol. Chem., August 29, 2003; 278(35): 32936 - 32942.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
J. Pan and W. J. Snell
Kinesin II and regulated intraflagellar transport of Chlamydomonas aurora protein kinase
J. Cell Sci., June 1, 2003; 116(11): 2179 - 2186.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
D. Watanabe, Y. Saijoh, S. Nonaka, G. Sasaki, Y. Ikawa, T. Yokoyama, and H. Hamada
The left-right determinant Inversin is a component of node monocilia and other 9+0 cilia
Development, May 1, 2003; 130(9): 1725 - 1734.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2002 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.