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A more recent version of this article appeared on February 1, 2002
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Submitted on August 29, 2001
Revised on October 31, 2001
Accepted on November 5, 2001
1 Department of Zoology, South Parks Road, Oxford, OX1 3PS, UK
* Corresponding author. E-mail address: stephen.kearsey{at}zoo.ox.ac.uk.
A critical event in eukaryotic DNA replication involves association of minichromosome maintenance (MCM2-7) proteins with origins, to form pre-replicative complexes (pre-RC) that are competent for initiation. The ability of mutants defective in MCM2-7 function to complete meiosis had suggested that pre-RC components could be irrelevant to pre-meiotic S phase. We show here that MCM2-7 proteins bind to chromatin in fission yeast cells preparing for meiosis, and during pre-meiotic S phase in a manner suggesting they in fact are required DNA replication in the meiotic cycle. This is confirmed by analysis of a degron mcm4 mutant which cannot carry out pre-meiotic DNA replication. Later in meiosis, Mcm4 chromatin association is blocked between meiotic nuclear divisions, presumably accounting for the absence of a second round of DNA replication. Together, these results emphasize similarity between replication mechanisms in mitotic and meiotic cell cycles.
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