![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
A more recent version of this article appeared on June 1, 2002
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Submitted on November 5, 2001
Revised on February 13, 2002
Accepted on February 21, 2002
1 Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01002
2 Department of Biology, Union College, Schenectady, NY
3 Wadsworth Center, N.Y. State Dept. of Health, Albany, NY
* Corresponding author. E-mail address: patw{at}bio.umass.edu.
The reorientation of the microtubule organizing center during cell migration into a wound in the monolayer was directly observed in living wound edge cells expressing 
-tubulin tagged with green fluorescent protein. Our results demonstrate that in CHO cells the centrosome reorients to a position in front of the nucleus, towards the wound edge, while in PtK cells the centrosome lags behind the nucleus during migration into the wound. In CHO cells the average rate of centrosome motion was faster than that of the nucleus; the converse was true in PtK cells. In both cell lines, centrosome motion was stochastic, with periods of rapid motion interspersed with periods of slower motion. Centrosome reorientation in CHO cells required dynamic microtubules, cytoplasmic dynein/dynactin activity and could be prevented by altering cell-cell or cell-substrate adhesion. Microtubule marking experiments using photoactivation of caged tubulin demonstrate that microtubules are transported in the direction of cell motility in both cell lines, but that in PtK cells microtubules move individually, while their movement is more coherent in CHO cells. Our data demonstrate that centrosome reorientation is not required for directed migration and that diverse cells utilize distinct mechanisms for remodeling the microtubule array during directed migration.
This article has been cited by other articles:
|
|
 |
|
  C. Sutterlin and A. Colanzi The Golgi and the centrosome: building a functional partnership J. Cell Biol., March 8, 2010; 188(5): 621 - 628. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. T. Bach, S. Creed, J. Zhong, M. Mahmassani, G. Schevzov, J. Stehn, L. N. Cowell, P. Naumanen, P. Lappalainen, P. W. Gunning, et al. Tropomyosin Isoform Expression Regulates the Transition of Adhesions To Determine Cell Speed and Direction Mol. Cell. Biol., March 15, 2009; 29(6): 1506 - 1514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Levy and E. L. F. Holzbaur Dynein drives nuclear rotation during forward progression of motile fibroblasts J. Cell Sci., October 1, 2008; 121(19): 3187 - 3195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Pouthas, P. Girard, V. Lecaudey, T. B. N. Ly, D. Gilmour, C. Boulin, R. Pepperkok, and E. G. Reynaud In migrating cells, the Golgi complex and the position of the centrosome depend on geometrical constraints of the substratum J. Cell Sci., July 15, 2008; 121(14): 2406 - 2414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kodani and C. Sutterlin The Golgi Protein GM130 Regulates Centrosome Morphology and Function Mol. Biol. Cell, February 1, 2008; 19(2): 745 - 753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chmielowiec, M. Borowiak, M. Morkel, T. Stradal, B. Munz, S. Werner, J. Wehland, C. Birchmeier, and W. Birchmeier c-Met is essential for wound healing in the skin J. Cell Biol., April 9, 2007; 177(1): 151 - 162. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Salaycik, C. J. Fagerstrom, K. Murthy, U. S. Tulu, and P. Wadsworth Quantification of microtubule nucleation, growth and dynamics in wound-edge cells J. Cell Sci., September 15, 2005; 118(18): 4113 - 4122. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lefebvre-Lavoie, J. G. Lussier, and C. L. Theoret Profiling of differentially expressed genes in wound margin biopsies of horses using suppression subtractive hybridization Physiol Genomics, July 14, 2005; 22(2): 157 - 170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Gillingham, A. H. Y. Tong, C. Boone, and S. Munro The GTPase Arf1p and the ER to Golgi cargo receptor Erv14p cooperate to recruit the golgin Rud3p to the cis-Golgi J. Cell Biol., October 25, 2004; 167(2): 281 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Finkelstein, W. Chang, P.-H. G. Chao, D. Gruber, A. Minden, C. T. Hung, and J. C. Bulinski Roles of microtubules, cell polarity and adhesion in electric-field-mediated motility of 3T3 fibroblasts J. Cell Sci., March 15, 2004; 117(8): 1533 - 1545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Pihan, J. Wallace, Y. Zhou, and S. J. Doxsey Centrosome Abnormalities and Chromosome Instability Occur Together in Pre-invasive Carcinomas Cancer Res., March 15, 2003; 63(6): 1398 - 1404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Yu, L. E. O'Brien, F. Wang, H. Bourne, K. E. Mostov, and M. M.P. Zegers Hepatocyte Growth Factor Switches Orientation of Polarity and Mode of Movement during Morphogenesis of Multicellular Epithelial Structures Mol. Biol. Cell, February 1, 2003; 14(2): 748 - 763. [Abstract] [Full Text] [PDF]
|
|
