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A more recent version of this article appeared on June 1, 2002
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Submitted on November 5, 2001
Revised on February 13, 2002
Accepted on February 21, 2002
1 Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01002
2 Department of Biology, Union College, Schenectady, NY
3 Wadsworth Center, N.Y. State Dept. of Health, Albany, NY
* Corresponding author. E-mail address: patw{at}bio.umass.edu.
The reorientation of the microtubule organizing center during cell migration into a wound in the monolayer was directly observed in living wound edge cells expressing 
-tubulin tagged with green fluorescent protein. Our results demonstrate that in CHO cells the centrosome reorients to a position in front of the nucleus, towards the wound edge, while in PtK cells the centrosome lags behind the nucleus during migration into the wound. In CHO cells the average rate of centrosome motion was faster than that of the nucleus; the converse was true in PtK cells. In both cell lines, centrosome motion was stochastic, with periods of rapid motion interspersed with periods of slower motion. Centrosome reorientation in CHO cells required dynamic microtubules, cytoplasmic dynein/dynactin activity and could be prevented by altering cell-cell or cell-substrate adhesion. Microtubule marking experiments using photoactivation of caged tubulin demonstrate that microtubules are transported in the direction of cell motility in both cell lines, but that in PtK cells microtubules move individually, while their movement is more coherent in CHO cells. Our data demonstrate that centrosome reorientation is not required for directed migration and that diverse cells utilize distinct mechanisms for remodeling the microtubule array during directed migration.
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