Molecular Biology of the Cell Call for Nominations: MBC Editor-in-Chief

Home Help [Feedback] [For Subscribers] [Archive] [Search] --
QUICK SEARCH:   [advanced]


     

MBC in Press, published online ahead of print November 18, 2002
Mol. Biol. Cell 10.1091/mbc.02-06-0089

A more recent version of this article appeared on February 1, 2003
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
02-06-0089v1
14/2/774    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Padma, P.
Right arrow Articles by Inaba, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Padma, P.
Right arrow Articles by Inaba, K.

Submitted on June 3, 2002
Accepted on October 21, 2002

Identification of a novel leucine-rich repeat protein as a component of flagellar radial spoke in the ascidian Ciona intestinalis

Potturi Padma1, Yuhkoh Satouh1, Ken-ichi Wakabayashi2, Akiko Hozumi1, Yuji Ushimaru1, Ritsu Kamiya3, and Kazuo Inaba4*

1 Asamushi Marine Biological Station, Graduate School of Science, Tohoku University, Sakamoto 9, Asamushi, Aomori, Aomori 039-3501, Japan
2 National Institute for Basic Biology, Okazaki 444-8585, Japan (present address: Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06030-3305)
3 National Institute for Basic Biology, Okazaki 444-8585, Japan; and Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan
4 Asamushi Marine Biological Station, Graduate School of Science, Tohoku University, Sakamoto 9, Asamushi, Aomori, Aomori 039-3501, Japan; and National Institute for Basic Biology, Okazaki 444-8585, Japan

* Corresponding author. E-mail address: inaba{at}biology.tohoku.ac.jp.

Axonemes are highly organized microtubule-based structures conserved in many eukaryotes. In an attempt to study axonemes by a proteomics approach, we selectively cloned cDNAs of axonemal proteins by immunoscreening the testis cDNA library from the ascidian Ciona intestinalis using an antiserum against whole axonemes. We report here a 37 kDa protein of which cDNA occurred most frequently among total positive clones. This protein, named LRR37, belongs to the class of SDS22+ leucine-rich repeat (LRR) family. LRR37 is different from the LRR outer arm dynein light chain reported in Chlamydomonas and sea urchin flagella, and thus represents a novel axonemal LRR protein. Immunoelectron microscopy using a polyclonal antibody against LRR37 showed that it is localized on the tip of the radial spoke, most likely on the spoke head. The LRR37 protein in fact appears to form a complex together with radial spoke protein 3 in a KI extract of the axonemes. These results suggest that LRR37 is a component of the radial spoke head and is involved in the interaction with other radial spoke components or proteins in the central pair projection.




This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
E. van Rooijen, R. H. Giles, E. E. Voest, C. van Rooijen, S. Schulte-Merker, and F. J. van Eeden
LRRC50, a Conserved Ciliary Protein Implicated in Polycystic Kidney Disease
J. Am. Soc. Nephrol., June 1, 2008; 19(6): 1128 - 1138.
[Full Text] [PDF]

Home page
 JCBHome page
C. Yang, H. A. Owen, and P. Yang
Dimeric heat shock protein 40 binds radial spokes for generating coupled power strokes and recovery strokes of 9 + 2 flagella
J. Cell Biol., January 28, 2008; 180(2): 403 - 415.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. R. Gaillard, L. A. Fox, J. M. Rhea, B. Craige, and W. S. Sale
Disruption of the A-Kinase Anchoring Domain in Flagellar Radial Spoke Protein 3 Results in Unregulated Axonemal cAMP-dependent Protein Kinase Activity and Abnormal Flagellar Motility
Mol. Biol. Cell, June 1, 2006; 17(6): 2626 - 2635.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
Y. Nishimune and H. Tanaka
Infertility Caused by Polymorphisms or Mutations in Spermatogenesis-Specific Genes
J Androl, May 1, 2006; 27(3): 326 - 334.
[Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. Hui, J. Lu, Y. Han, and S. H. Pilder
The Mouse t Complex Gene Tsga2, Encoding Polypeptides Located in the Sperm Tail and Anterior Acrosome, Maps to a Locus Associated with Sperm Motility and Sperm-Egg Interaction Abnormalities
Biol Reprod, April 1, 2006; 74(4): 633 - 643.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
P. Yang, D. R. Diener, C. Yang, T. Kohno, G. J. Pazour, J. M. Dienes, N. S. Agrin, S. M. King, W. S. Sale, R. Kamiya, et al.
Radial spoke proteins of Chlamydomonas flagella
J. Cell Sci., March 15, 2006; 119(6): 1165 - 1174.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. Yang, M. M. Compton, and P. Yang
Dimeric Novel HSP40 Is Incorporated into the Radial Spoke Complex during the Assembly Process in Flagella
Mol. Biol. Cell, February 1, 2005; 16(2): 637 - 648.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
P. Yang, C. Yang, and W. S. Sale
Flagellar Radial Spoke Protein 2 Is a Calmodulin Binding Protein Required for Motility in Chlamydomonas reinhardtii
Eukaryot. Cell, February 1, 2004; 3(1): 72 - 81.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Copyright © 2002 by The American Society for Cell Biology. Terms of copyright protection, warranties, and disclaimers.