QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Cikaluk, D. E. Articles by Hobman, T. C. Search for Related Content PubMed PubMed Citation Articles by Cikaluk, D. E. Articles by Hobman, T. C.

Vol. 10, Issue 10, 3357-3372, October 1999

GERp95, a Membrane-associated Protein that Belongs to a Family of Proteins Involved in Stem Cell Differentiation

Darren E. Cikaluk,^* Nasser Tahbaz,^* Linda C. Hendricks, Gabriel E. DiMattia, Dave Hansen,^¶ Dave Pilgrim,^¶ and Tom C. Hobman^*

Departments of  ^*Cell Biology and  ^¶Biological Sciences, University of Alberta, Edmonton, AB, T6G 2H7, Canada;  Biological Process Sciences, SmithKline Beecham Pharmaceuticals, King of Prussia, Pennsylvania 19406; and  London Regional Cancer Centre, London, Ontario N6A 4L6 Canada

A panel of mAbs was elicited against intracellular membrane fractions from rat pancreas. One of the antibodies reacted with a 95-kDa protein that localizes primarily to the Golgi complex or the endoplasmic reticulum (ER), depending on cell type. The corresponding cDNA was cloned and sequenced and found to encode a protein of 97.6 kDa that we call GERp95 (Golgi ER protein 95 kDa). The protein copurifies with intracellular membranes but does not contain hydrophobic regions that could function as signal peptides or transmembrane domains. Biochemical analysis suggests that GERp95 is a cytoplasmically exposed peripheral membrane protein that exists in a protease-resistant complex. GERp95 belongs to a family of highly conserved proteins in metazoans and Schizosaccharomyces pombe. It has recently been determined that plant and Drosophila homologues of GERp95 are important for controlling the differentiation of stem cells (Bohmert et al., 1998; Cox et al., 1998; Moussian et al., 1998). In Caenorhabditis elegans, there are at least 20 members of this protein family. To this end, we have used RNA interference to show that the GERp95 orthologue in C. elegans is important for maturation of germ-line stem cells in the gonad. GERp95 and related proteins are an emerging new family of proteins that have important roles in metazoan development. The present study suggests that these proteins may exert their effects on cell differentiation from the level of intracellular membranes.

---

   Corresponding author. E-mail address: thobman{at}anat.med.ualberta.ca.

---

Molecular Biology of the Cell
Vol. 10, 3357-3372, October 1999
Copyright © 1999 by The American Society for Cell Biology

This article has been cited by other articles:

				G. S. Tan, B. G. Garchow, X. Liu, J. Yeung, J. P. Morris IV, T. L. Cuellar, M. T. McManus, and M. Kiriakidou Expanded RNA-binding activities of mammalian Argonaute 2 Nucleic Acids Res., December 1, 2009; 37(22): 7533 - 7545. [Abstract] [Full Text] [PDF]

				M.-C. Didiot, M. Subramanian, E. Flatter, J.-L. Mandel, and H. Moine Cells Lacking the Fragile X Mental Retardation Protein (FMRP) have Normal RISC Activity but Exhibit Altered Stress Granule Assembly Mol. Biol. Cell, January 1, 2009; 20(1): 428 - 437. [Abstract] [Full Text] [PDF]

				C. Vijayasarathy, Y. Takada, Y. Zeng, R. A. Bush, and P. A. Sieving Retinoschisin Is a Peripheral Membrane Protein with Affinity for Anionic Phospholipids and Affected by Divalent Cations Invest. Ophthalmol. Vis. Sci., March 1, 2007; 48(3): 991 - 1000. [Abstract] [Full Text] [PDF]

				E. MANIATAKI and Z. MOURELATOS Human mitochondrial tRNAMet is exported to the cytoplasm and associates with the Argonaute 2 protein RNA, June 1, 2005; 11(6): 849 - 852. [Abstract] [Full Text] [PDF]

				A. H. S. Hall, J. Wan, E. E. Shaughnessy, B. Ramsay Shaw, and K. A. Alexander RNA interference using boranophosphate siRNAs: structure-activity relationships Nucleic Acids Res., November 15, 2004; 32(20): 5991 - 6000. [Abstract] [Full Text] [PDF]

				J. B. Carmichael, P. Provost, K. Ekwall, and T. C. Hobman Ago1 and Dcr1, Two Core Components of the RNA Interference Pathway, Functionally Diverge from Rdp1 in Regulating Cell Cycle Events in Schizosaccharomyces pombe Mol. Biol. Cell, March 1, 2004; 15(3): 1425 - 1435. [Abstract] [Full Text] [PDF]

				H. Shi, A. Djikeng, C. Tschudi, and E. Ullu Argonaute Protein in the Early Divergent Eukaryote Trypanosoma brucei: Control of Small Interfering RNA Accumulation and Retroposon Transcript Abundance Mol. Cell. Biol., January 1, 2004; 24(1): 420 - 427. [Abstract] [Full Text] [PDF]

				M. A. Carmell, Z. Xuan, M. Q. Zhang, and G. J. Hannon The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis Genes & Dev., November 1, 2002; 16(21): 2733 - 2742. [Full Text] [PDF]

				D. S. Schwarz and P. D. Zamore Why do miRNAs live in the miRNP? Genes & Dev., May 1, 2002; 16(9): 1025 - 1031. [Full Text] [PDF]

				N. Tahbaz, J. B. Carmichael, and T. C. Hobman GERp95 Belongs to a Family of Signal-transducing Proteins and Requires Hsp90 Activity for Stability and Golgi Localization J. Biol. Chem., November 9, 2001; 276(46): 43294 - 43299. [Abstract] [Full Text] [PDF]

				M. D. Beatch and T. C. Hobman Rubella Virus Capsid Associates with Host Cell Protein p32 and Localizes to Mitochondria J. Virol., June 15, 2000; 74(12): 5569 - 5576. [Abstract] [Full Text] [PDF]