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Vol. 10, Issue 10, 3389-3400, October 1999
and
Division of Yeast Genetics, National Institute for Medical
Research, The Ridgeway, Mill Hill, London NW7 1AA, United Kingdom
We previously isolated the SKN7 gene in a screen
designed to isolate new components of the G1-S cell cycle transcription
machinery in budding yeast. We have now found that Skn7 associates with Mbp1, the DNA-binding component of the G1-S transcription factor DSC1/MBF. SKN7 and MBP1 show several
genetic interactions. Skn7 overexpression is lethal and is suppressed
by a mutation in MBP1. Similarly, high overexpression of
Mbp1 is lethal and can be suppressed by skn7 mutations.
SKN7 is also required for MBP1 function
in a mutant compromised for G1-specific transcription. Gel-retardation assays indicate that Skn7 is not an integral part of MBF. However, a
physical interaction between Skn7 and Mbp1 was detected using two-hybrid assays and GST pulldowns. Thus, Skn7 and Mbp1 seem to form a
transcription factor independent of MBF. Genetic data suggest that this
new transcription factor could be involved in the bud-emergence process.
Department
of Biochemistry and Genetics, Medical School, University of Newcastle,
Newcastle-upon-Tyne NE2 4HH, United Kingdom.
Corresponding author. E-mail address:
ljohnst{at}nimr.mrc.ac.uk.
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