![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 11, 3689-3703, November 1999
Department of Biochemistry and Biophysics, University of
California, San Francisco, San Francisco, California 94143-0448
We have performed a genetic and biochemical analysis of the
SPO12 gene, which regulates meiotic nuclear divisions in
budding yeast. When sporulated, spo12 mutants undergo a
single meiotic nuclear division most closely resembling meiosis
II. We observed that Spo12 protein is localized to the nucleus
during both meiotic divisions and that Clb1-Cdc28, Clb3-Cdc28,
Clb4-Cdc28, and Clb5-Cdc28 kinase activities during meiosis were not
affected by a spo12 mutation. Using two-hybrid analysis,
we identified several genes, three of which are meiotically induced,
that may code for proteins that interact with Spo12p. We also observed
that two genes, BNS1 (Bypasses Need for
Spo12p), which has homology to SPO12, and
SPO13, whose mutant phenotype is like that of
spo12, can partially suppress the meiotic defect of
spo12 mutants when overexpressed. We found that Spo12p
is also localized to the nucleus in vegetative cells and that its level
peaks during G2/M. We observed that a spo12 mutation is
synthetically lethal in vegetative cells with a mutation in
HCT1, a gene necessary for cells to exit mitosis,
suggesting that Spo12p may have a role in exit from mitosis.
Corresponding author. E-mail address:
ira{at}cgl.ucsf.edu.
This article has been cited by other articles:
|
|
 |
|
  K. L. Auld, A. L. Hitchcock, H. K. Doherty, S. Frietze, L. S. Huang, and P. A. Silver The Conserved ATPase Get3/Arr4 Modulates the Activity of Membrane-Associated Proteins in Saccharomyces cerevisiae Genetics, September 1, 2006; 174(1): 215 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. S. Huang, H. K. Doherty, and I. Herskowitz The Smk1p MAP kinase negatively regulates Gsc2p, a 1,3-{beta}-glucan synthase, during spore wall morphogenesis in Saccharomyces cerevisiae PNAS, August 30, 2005; 102(35): 12431 - 12436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Visintin, F. Stegmeier, and A. Amon The Role of the Polo Kinase Cdc5 in Controlling Cdc14 Localization Mol. Biol. Cell, November 1, 2003; 14(11): 4486 - 4498. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. R. Benjamin, C. Zhang, K. M. Shokat, and I. Herskowitz Control of landmark events in meiosis by the CDK Cdc28 and the meiosis-specific kinase Ime2 Genes & Dev., June 15, 2003; 17(12): 1524 - 1539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Shonn, R. McCarroll, and A. W. Murray Spo13 protects meiotic cohesin at centromeres in meiosis I Genes & Dev., July 1, 2002; 16(13): 1659 - 1671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Borgne, H. Murakami, J. Ayte, and P. Nurse The G1/S Cyclin Cig2p during Meiosis in Fission Yeast Mol. Biol. Cell, June 1, 2002; 13(6): 2080 - 2090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. van Hemert, G. E. M. Lamers, D. C. G. Klein, T. H. Oosterkamp, H. Y. Steensma, and G. P. H. van Heusden From the Cover: The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies PNAS, April 16, 2002; 99(8): 5390 - 5393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Shah, S. Jensen, L. M. Frenz, A. L. Johnson, and L. H. Johnston The Spo12 Protein of Saccharomyces cerevisiae: A Regulator of Mitotic Exit Whose Cell Cycle-Dependent Degradation Is Mediated by the Anaphase-Promoting Complex Genetics, November 1, 2001; 159(3): 965 - 980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. H. Rutkowski and R. E. Esposito Recombination Can Partially Substitute for SPO13 in Regulating Meiosis I in Budding Yeast Genetics, August 1, 2000; 155(4): 1607 - 1621. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. R. Chaves and G. Blobel Nuclear Import of Spo12p, a Protein Essential for Meiosis J. Biol. Chem., May 18, 2001; 276(21): 17712 - 17717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. van Hemert, G. E. M. Lamers, D. C. G. Klein, T. H. Oosterkamp, H. Y. Steensma, and G. P. H. van Heusden From the Cover: The Saccharomyces cerevisiae Fin1 protein forms cell cycle-specific filaments between spindle pole bodies PNAS, April 16, 2002; 99(8): 5390 - 5393. [Abstract] [Full Text] [PDF]
|
|
