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Vol. 10, Issue 11, 3705-3715, November 1999

Cell Cycle-regulated Transcription in Fission Yeast: Cdc10-Res Protein Interactions during the Cell Cycle and Domains Required for Regulated Transcription

Simon Whitehall,* Peter Stacey,dagger Keren Dawson,dagger Dagger and Nic Jonesdagger Dagger §

 dagger Laboratory of Gene Regulation, Imperial Cancer Research Fund, London WC2A 3PX, United Kingdom; and  *School of Biochemistry and Genetics, The Medical School, The University of Newcastle, Newcastle-upon-Tyne NE2 4HH, United Kingdom

In Schizosaccharomyces pombe the MBF (DSC1) complex mediates transcriptional activation at Start and is composed of a common subunit called Cdc10 in combination with two alternative DNA-binding partners, Res1 and Res2. It has been suggested that a high-activity MBF complex (at G1/S) is switched to a low-activity complex (in G2) by the incorporation of the negative regulatory subunit Res2. We have analyzed MBF protein-protein interactions and find that both Res proteins are associated with Cdc10 throughout the cell cycle, arguing against this model. Furthermore we demonstrate that Res2 is capable of interacting with a mutant form of Cdc10 that has high transcriptional activity. It has been shown previously that both Res proteins are required for periodic cell cycle-regulated transcription. Therefore a series of Res1-Res2 hybrid molecules was used to determine the domains that are specifically required to regulate periodic transcription. In Res2 the nature of the C-terminal region is critical, and in both Res1 and Res2, a domain overlapping the N-terminal ankyrin repeat and a recently identified activation domain is important for mediating cell cycle-regulated transcription.

Dagger    Present address: The Paterson Institute for Cancer Research, Christie Hospital NHS Trust, Wilmslow Road, Manchester M20 4BX, UK.
§   Corresponding author. E-mail address: njones{at}picr.man.ac.uk.

Molecular Biology of the Cell
Vol. 10, 3705-3715, November 1999
Copyright © 1999 by The American Society for Cell Biology


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