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Vol. 10, Issue 11, 3815-3824, November 1999

Nuclear Domains Enriched in RNA 3'-processing Factors Associate with Coiled Bodies and Histone Genes in a Cell Cycle-dependent Manner

Wouter Schul,^* Ineke van der Kraan,^* A. Gregory Matera, Roel van Driel,^* and Luitzen de Jong^*

 ^*E. C. Slater Instituut, University of Amsterdam, BioCentrum Amsterdam, 1018 TV Amsterdam, The Netherlands; and  Department of Genetics, Center for Human Genetics and Program in Cell Biology, Case Western Reserve University, Cleveland, Ohio 44106-4955

Nuclear domains, called cleavage bodies, are enriched in the RNA 3'-processing factors CstF 64 kDa and and CPSF 100 kDa. Cleavage bodies have been found either overlapping with or adjacent to coiled bodies. To determine whether the spatial relationship between cleavage bodies and coiled bodies was influenced by the cell cycle, we performed cell synchronization studies. We found that in G1 phase cleavage bodies and coiled bodies were predominantly coincident, whereas in S phase they were mostly adjacent to each other. In G2 cleavage bodies were often less defined or absent, suggesting that they disassemble at this point in the cell cycle. A small number of genetic loci have been reported to be juxtaposed to coiled bodies, including the genes for U1 and U2 small nuclear RNA as well as the two major histone gene clusters. Here we show that cleavage bodies do not overlap with small nuclear RNA genes but do colocalize with the histone genes next to coiled bodies. These findings demonstrate that the association of cleavage bodies and coiled bodies is both dynamic and tightly regulated and suggest that the interaction between these nuclear neighbors is related to the cell cycle-dependent expression of histone genes.

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   Corresponding author. E-mail address: a311luit{at}chem.uva.nl.

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Molecular Biology of the Cell
Vol. 10, 3815-3824, November 1999
Copyright © 1999 by The American Society for Cell Biology

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