![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 11, 3835-3848, November 1999
Institute for Cancer Research, The Norwegian Radium Hospital,
Montebello, 0310 Oslo, Norway
Endocytic uptake and intracellular transport of acidic FGF was
studied in cells transfected with FGF receptor 4 (FGFR4). Acidification of the cytosol to block endocytic uptake from coated pits did not
inhibit endocytosis of the growth factor in COS cells transfected with
FGFR4, indicating that it is to a large extent taken up by an
alternative endocytic pathway. Fractionation of the cells demonstrated that part of the growth factor receptor was present in a low-density, caveolin-containing fraction, but we were unable to demonstrate binding
to caveolin in immunoprecipitation studies. Upon treatment of the cells
with acidic FGF, the activated receptor, together with the growth
factor, moved to a juxtanuclear compartment, which was identified as
the recycling endosome compartment. When the cells were lysed with
Triton X-100,
3-([3-chloramidopropyl]dimethylammonio)-2-hydroxy-1-propanesulfonate, or 2-octyl glucoside, almost all surface-exposed and endocytosed FGFR4
was solubilized, but only a minor fraction of the total FGFR4 in the
cells was found in the soluble fraction. The data indicate that the
major part of FGFR4 is anchored to detergent-insoluble structures,
presumably cytoskeletal elements associated with the recycling endosome compartment.
This article has been cited by other articles:
|
|
 |
|
  V. Sorensen, A. Wiedlocha, E. M. Haugsten, D. Khnykin, J. Wesche, and S. Olsnes Different abilities of the four FGFRs to mediate FGF-1 translocation are linked to differences in the receptor C-terminal tail J. Cell Sci., October 15, 2006; 119(20): 4332 - 4341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Zhu, W. C. Xiong, and L. Mei Lipid rafts serve as a signaling platform for nicotinic acetylcholine receptor clustering. J. Neurosci., May 3, 2006; 26(18): 4841 - 4851. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Wesche, J. Malecki, A. Wiedlocha, C. S. Skjerpen, P. Claus, and S. Olsnes FGF-1 and FGF-2 Require the Cytosolic Chaperone Hsp90 for Translocation into the Cytosol and the Cell Nucleus J. Biol. Chem., April 21, 2006; 281(16): 11405 - 11412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Haugsten, V. Sorensen, A. Brech, S. Olsnes, and J. Wesche Different intracellular trafficking of FGF1 endocytosed by the four homologous FGF receptors J. Cell Sci., September 1, 2005; 118(17): 3869 - 3881. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Wiedlocha, T. Nilsen, J. Wesche, V. Sorensen, J. Malecki, E. Marcinkowska, and S. Olsnes Phosphorylation-regulated Nucleocytoplasmic Trafficking of Internalized Fibroblast Growth Factor-1 Mol. Biol. Cell, February 1, 2005; 16(2): 794 - 810. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Suzuki, T. Numakawa, K. Shimazu, H. Koshimizu, T. Hara, H. Hatanaka, L. Mei, B. Lu, and M. Kojima BDNF-induced recruitment of TrkB receptor into neuronal lipid rafts: roles in synaptic modulation J. Cell Biol., December 20, 2004; 167(6): 1205 - 1215. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Tkachenko, E. Lutgens, R.-V. Stan, and M. Simons Fibroblast growth factor 2 endocytosis in endothelial cells proceed via syndecan-4-dependent activation of Rac1 and a Cdc42-dependent macropinocytic pathway J. Cell Sci., July 1, 2004; 117(15): 3189 - 3199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Sorensen, A. Brech, D. Khnykin, E. Kolpakova, L. Citores, and S. Olsnes Deletion mutant of FGFR4 induces onion-like membrane structures in the nucleus J. Cell Sci., May 1, 2004; 117(9): 1807 - 1819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Malecki, J. Wesche, C. S. Skjerpen, A. Wiedlocha, and S. Olsnes Translocation of FGF-1 and FGF-2 across Vesicular Membranes Occurs during G1-Phase by a Common Mechanism Mol. Biol. Cell, February 1, 2004; 15(2): 801 - 814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Cho, C. Guo, M. Torello, G. P. Lunstrum, T. Iwata, C. Deng, and W. A. Horton Defective lysosomal targeting of activated fibroblast growth factor receptor 3 in achondroplasia PNAS, January 13, 2004; 101(2): 609 - 614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rodemer, T.-P. Thai, B. Brugger, T. Kaercher, H. Werner, K.-A. Nave, F. Wieland, K. Gorgas, and W. W. Just Inactivation of ether lipid biosynthesis causes male infertility, defects in eye development and optic nerve hypoplasia in mice Hum. Mol. Genet., August 1, 2003; 12(15): 1881 - 1895. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Olsnes, O. Klingenberg, and A. Wiedlocha Transport of Exogenous Growth Factors and Cytokines to the Cytosol and to the Nucleus Physiol Rev, January 1, 2003; 83(1): 163 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kohl, M. Antoine, K. Reimers, and P. Kiefer FGF3 Attached to a Phosholipid Membrane Anchor Gains a High Transforming Capacity. IMPLICATIONS OF MICRODOMAINS FOR FGF3 CELL TRANSFORMATION J. Biol. Chem., August 30, 2002; 277(36): 32760 - 32767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Iakhiaev, U. R. Pendurthi, and L. V. M. Rao Active Site Blockade of Factor VIIa Alters Its Intracellular Distribution J. Biol. Chem., November 30, 2001; 276(49): 45895 - 45901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L Citores, D Khnykin, V Sorensen, J Wesche, O Klingenberg, A Wiedlocha, and S Olsnes Modulation of intracellular transport of acidic fibroblast growth factor by mutations in the cytoplasmic receptor domain J. Cell Sci., January 5, 2001; 114(9): 1677 - 1689. [Abstract] [PDF]
|
|
|
|
|
|
O. Klingenberg, A. Wiedlocha, L. Citores, and S. Olsnes Requirement of Phosphatidylinositol 3-Kinase Activity for Translocation of Exogenous aFGF to the Cytosol and Nucleus J. Biol. Chem., April 21, 2000; 275(16): 11972 - 11980. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O Klingenberg, A Wiedlocha, A Rapak, D Khnykin, L Citores, and S Olsnes Requirement for C-terminal end of fibroblast growth factor receptor 4 in translocation of acidic fibroblast growth factor to cytosol and nucleus J. Cell Sci., January 5, 2000; 113(10): 1827 - 1838. [Abstract] [PDF]
|
|
