![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 11, 3863-3876, November 1999
Department of Pharmacological Sciences and Institute for Cell and
Developmental Biology, State University of New York, Stony Brook, New
York 11794-8651
Phosphatidylcholine (PC) is a major source of lipid-derived second
messenger molecules that function as both intracellular and
extracellular signals. PC-specific phospholipase D (PLD) and phosphatidic acid phosphohydrolase (PAP) are two pivotal enzymes in
this signaling system, and they act in series to generate the biologically active lipids phosphatidic acid (PA) and diglyceride. The
identity of the PAP enzyme involved in PLD-mediated signal transduction
is unclear. We provide the first evidence for a functional role of a
type 2 PAP, PAP2b, in the metabolism of PLD-generated PA. Our data
indicate that PAP2b localizes to regions of the cell in which PC
hydrolysis by PLD is taking place. Using a newly developed PAP2b-specific antibody, we have characterized the expression, posttranslational modification, and localization of endogenous PAP2b.
Glycosylation and localization of PAP2b appear to be cell type and
tissue specific. Biochemical fractionation and immunoprecipitation analyses revealed that PAP2b and PLD2 activities are present in caveolin-1-enriched detergent-resistant membrane microdomains. We
found that PLD2 and PAP2b act sequentially to generate diglyceride within this specialized membrane compartment. The unique lipid composition of these membranes may provide a selective environment for
the regulation and actions of enzymes involved in signaling through PC hydrolysis.
This article has been cited by other articles:
|
|
 |
|
  S. Sethu, G. Mendez-Corao, and A. J. Melendez Phospholipase D1 Plays a Key Role in TNF-{alpha} Signaling J. Immunol., May 1, 2008; 180(9): 6027 - 6034. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Fernandez-Ulibarri, M. Vilella, F. Lazaro-Dieguez, E. Sarri, S. E. Martinez, N. Jimenez, E. Claro, I. Merida, K. N.J. Burger, and G. Egea Diacylglycerol Is Required for the Formation of COPI Vesicles in the Golgi-to-ER Transport Pathway Mol. Biol. Cell, September 1, 2007; 18(9): 3250 - 3263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. J. Sigal, O. A. Quintero, R. E. Cheney, and A. J. Morris Cdc42 and ARP2/3-independent regulation of filopodia by an integral membrane lipid-phosphatase-related protein J. Cell Sci., January 15, 2007; 120(2): 340 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Pilquil, J. Dewald, A. Cherney, I. Gorshkova, G. Tigyi, D. English, V. Natarajan, and D. N. Brindley Lipid Phosphate Phosphatase-1 Regulates Lysophosphatidate-induced Fibroblast Migration by Controlling Phospholipase D2-dependent Phosphatidate Generation J. Biol. Chem., December 15, 2006; 281(50): 38418 - 38429. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kai, F. Sakane, Y.-J. Jia, S.-i. Imai, S. Yasuda, and H. Kanoh Lipid Phosphate Phosphatases 1 and 3 Are Localized in Distinct Lipid Rafts J. Biochem., November 1, 2006; 140(5): 677 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J. Park, M. Suh, B. Thomson, A. B.R. Thomson, K. S. Ramanujam, and M. T. Clinin Dietary ganglioside decreases cholesterol content, caveolin expression and inflammatory mediators in rat intestinal microdomains Glycobiology, October 1, 2005; 15(10): 935 - 942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Hiramatsu, H. Sonoda, Y. Takanezawa, R. Morikawa, M. Ishida, K. Kasahara, Y. Sanai, R. Taguchi, J. Aoki, and H. Arai Biochemical and Molecular Characterization of Two Phosphatidic Acid-selective Phospholipase A1s, mPA-PLA1{alpha} and mPA-PLA1{beta} J. Biol. Chem., December 5, 2003; 278(49): 49438 - 49447. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Smyth, V. A. Sciorra, Y. J. Sigal, Z. Pamuklar, Z. Wang, Y. Xu, G. D. Prestwich, and A. J. Morris Lipid Phosphate Phosphatases Regulate Lysophosphatidic Acid Production and Signaling in Platelets: STUDIES USING CHEMICAL INHIBITORS OF LIPID PHOSPHATE PHOSPHATASE ACTIVITY J. Biol. Chem., October 31, 2003; 278(44): 43214 - 43223. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Luquain, A. Singh, L. Wang, V. Natarajan, and A. J. Morris Role of phospholipase D in agonist-stimulated lysophosphatidic acid synthesis by ovarian cancer cells J. Lipid Res., October 1, 2003; 44(10): 1963 - 1975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Escalante-Alcalde, L. Hernandez, H. Le Stunff, R. Maeda, H.-S. Lee, Jr-Gang-Cheng, V. A. Sciorra, I. Daar, S. Spiegel, A. J. Morris, et al. The lipid phosphatase LPP3 regulates extra-embryonic vasculogenesis and axis patterning Development, October 1, 2003; 130(19): 4623 - 4637. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Tanyi, A. J. Morris, J. K. Wolf, X. Fang, Y. Hasegawa, R. Lapushin, N. Auersperg, Y. J. Sigal, R. A. Newman, E. A. Felix, et al. The Human Lipid Phosphate Phosphatase-3 Decreases the Growth, Survival, and Tumorigenesis of Ovarian Cancer Cells: Validation of the Lysophosphatidic Acid Signaling Cascade as a Target for Therapy in Ovarian Cancer Cancer Res., March 1, 2003; 63(5): 1073 - 1082. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nanjundan and F. Possmayer Pulmonary phosphatidic acid phosphatase and lipid phosphate phosphohydrolase Am J Physiol Lung Cell Mol Physiol, January 1, 2003; 284(1): L1 - L23. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A. Sciorra, S. A. Rudge, J. Wang, S. McLaughlin, J. Engebrecht, and A. J. Morris Dual role for phosphoinositides in regulation of yeast and mammalian phospholipase D enzymes J. Cell Biol., December 23, 2002; 159(6): 1039 - 1049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nanjundan and F. Possmayer Molecular cloning and expression of pulmonary lipid phosphate phosphohydrolases Am J Physiol Lung Cell Mol Physiol, December 1, 2001; 281(6): L1484 - L1493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Rudge, T. R. Pettitt, C. Zhou, M. J. O. Wakelam, and J. Engebrecht SPO14 Separation-of-Function Mutations Define Unique Roles for Phospholipase D in Secretion and Cellular Differentiation in Saccharomyces cerevisiae Genetics, August 1, 2001; 158(4): 1431 - 1444. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. J. Oprins, C. van der Burg, H. P. Meijer, T. Munnik, and J. A. Groot PLD pathway involved in carbachol-induced Cl{-} secretion: possible role of TNF-{alpha} Am J Physiol Cell Physiol, April 1, 2001; 280(4): C789 - C795. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Imai, T. Furui, T. Tamaya, and G. B. Mills A Gonadotropin-Releasing Hormone-Responsive Phosphatase Hydrolyses Lysophosphatidic Acid within the Plasma Membrane of Ovarian Cancer Cells J. Clin. Endocrinol. Metab., September 1, 2000; 85(9): 3370 - 3375. [Abstract] [Full Text]
|
|
