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Vol. 10, Issue 11, 3877-3890, November 1999
and
*Division of Biology and Medicine, Brown University, Providence,
Rhode Island 02912; and The nucleolar localization elements (NoLEs) of U17 small nucleolar
RNA (snoRNA), which is essential for rRNA processing and belongs to the
box H/ACA snoRNA family, were analyzed by fluorescence microscopy.
Injection of mutant U17 transcripts into Xenopus laevis oocyte nuclei revealed that deletion of stems 1, 2, and 4 of U17 snoRNA
reduced but did not prevent nucleolar localization. The deletion of
stem 3 had no adverse effect. Therefore, the hairpins of the
hairpin-hinge-hairpin-tail structure formed by these stems are not
absolutely critical for nucleolar localization of U17, nor are
sequences within stems 1, 3, and 4, which may tether U17 to the rRNA
precursor by base pairing. In contrast, box H and box ACA are
major NoLEs; their combined substitution or deletion abolished
nucleolar localization of U17 snoRNA. Mutation of just box H or just
the box ACA region alone did not fully abolish the nucleolar
localization of U17. This indicates that the NoLEs of the box H/ACA
snoRNA family function differently from the bipartite NoLEs (conserved
boxes C and D) of box C/D snoRNAs, where mutation of either box alone
prevents nucleolar localization.
Dipartimento di Biologia,
Universita di Roma "Tor Vergata," 00133 Rome, Italy
Corresponding author. E-mail
address: Susan_Gerbi{at}Brown.edu.
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