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Vol. 10, Issue 11, 3877-3890, November 1999

Box H and Box ACA Are Nucleolar Localization Elements of U17 Small Nucleolar RNA

Thilo Sascha Lange,* Michael Ezrokhi,* Francesco Amaldi,dagger and Susan A. Gerbi*Dagger

 *Division of Biology and Medicine, Brown University, Providence, Rhode Island 02912; and  dagger Dipartimento di Biologia, Universita di Roma "Tor Vergata," 00133 Rome, Italy

The nucleolar localization elements (NoLEs) of U17 small nucleolar RNA (snoRNA), which is essential for rRNA processing and belongs to the box H/ACA snoRNA family, were analyzed by fluorescence microscopy. Injection of mutant U17 transcripts into Xenopus laevis oocyte nuclei revealed that deletion of stems 1, 2, and 4 of U17 snoRNA reduced but did not prevent nucleolar localization. The deletion of stem 3 had no adverse effect. Therefore, the hairpins of the hairpin-hinge-hairpin-tail structure formed by these stems are not absolutely critical for nucleolar localization of U17, nor are sequences within stems 1, 3, and 4, which may tether U17 to the rRNA precursor by base pairing. In contrast, box H and box ACA are major NoLEs; their combined substitution or deletion abolished nucleolar localization of U17 snoRNA. Mutation of just box H or just the box ACA region alone did not fully abolish the nucleolar localization of U17. This indicates that the NoLEs of the box H/ACA snoRNA family function differently from the bipartite NoLEs (conserved boxes C and D) of box C/D snoRNAs, where mutation of either box alone prevents nucleolar localization.

Dagger    Corresponding author. E-mail address: Susan_Gerbi{at}Brown.edu.

Molecular Biology of the Cell
Vol. 10, 3877-3890, November 1999
Copyright © 1999 by The American Society for Cell Biology


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