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Vol. 10, Issue 12, 4121-4133, December 1999
*Department of Cell Biology and Budding yeast grow asymmetrically by the polarized delivery of
proteins and lipids to specific sites on the plasma membrane. This
requires the coordinated polarization of the actin cytoskeleton and the
secretory apparatus. We identified Rho3 on the basis of its genetic
interactions with several late-acting secretory genes. Mutational
analysis of the Rho3 effector domain reveals three distinct functions
in cell polarity: regulation of actin polarity, transport of exocytic
vesicles from the mother cell to the bud, and docking and fusion of
vesicles with the plasma membrane. We provide evidence that the vesicle
delivery function of Rho3 is mediated by the unconventional myosin Myo2
and that the docking and fusion function is mediated by the exocyst
component Exo70. These data suggest that Rho3 acts as a key regulator
of cell polarity and exocytosis, coordinating several distinct events
for delivery of proteins to specific sites on the cell surface.
Graduate Program in
Molecular Biology, Cell Biology, and Genetics, Weill Medical College of
Cornell University, New York, New York 10021
Corresponding author. E-mail address:
pjbrennw{at}mail.med.cornell.edu.
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