![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 12, 4311-4326, December 1999
Columbia University College of Physicians and Surgeons, Department
of Biochemistry and Molecular Biophysics, New York, New York 10032
The Caenorhabditis elegans oocyte is a highly
amenable system for forward and reverse genetic analysis of
receptor-mediated endocytosis. We describe the use of transgenic
strains expressing a vitellogenin::green fluorescent protein
(YP170::GFP) fusion to monitor yolk endocytosis by the
C. elegans oocyte in vivo. This YP170::GFP
reporter was used to assay the functions of C. elegans
predicted proteins homologous to vertebrate endocytosis factors using
RNA-mediated interference. We show that the basic components and
pathways of endocytic trafficking are conserved between C.
elegans and vertebrates, and that this system can be used to
test the endocytic functions of any new gene. We also used the
YP170::GFP assay to identify rme
(receptor-mediated endocytosis) mutants. We describe a new member of
the low-density lipoprotein receptor superfamily, RME-2,
identified in our screens for endocytosis defective mutants. We show
that RME-2 is the C. elegans yolk receptor.
This article has been cited by other articles:
|
|
 |
|
  L. Nilsson, B. Conradt, A.-F. Ruaud, C. C.-H. Chen, J. Hatzold, J.-L. Bessereau, B. D. Grant, and S. Tuck Caenorhabditis elegans num-1 Negatively Regulates Endocytic Recycling Genetics, May 1, 2008; 179(1): 375 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Mishra, A. Jha, A. L. Steinhauser, V. A. Kokoza, C. H. Washabaugh, A. S. Raikhel, W. A. Foster, and L. M. Traub Internalization of LDL-receptor superfamily yolk-protein receptors during mosquito oogenesis involves transcriptional regulation of PTB-domain adaptors J. Cell Sci., April 15, 2008; 121(8): 1264 - 1274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Han, P. Sundaram, H. Kenjale, J. Grantham, and L. Timmons The Caenorhabditis elegans rsd-2 and rsd-6 Genes Are Required for Chromosome Functions During Exposure to Unfavorable Environments Genetics, April 1, 2008; 178(4): 1875 - 1893. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Lu, Y. Zhang, T. Hu, P. Guo, W. Li, and X. Wang C. elegans Rab GTPase 2 is required for the degradation of apoptotic cells Development, March 15, 2008; 135(6): 1069 - 1080. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-H. Lee, M. Ohmachi, S. Arur, S. Nayak, R. Francis, D. Church, E. Lambie, and T. Schedl Multiple Functions and Dynamic Activation of MPK-1 Extracellular Signal-Regulated Kinase Signaling in Caenorhabditis elegans Germline Development Genetics, December 1, 2007; 177(4): 2039 - 2062. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Samuelson, C. E. Carr, and G. Ruvkun Gene activities that mediate increased life span of C. elegans insulin-like signaling mutants Genes & Dev., November 15, 2007; 21(22): 2976 - 2994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Currie, B. King, A. L. Lawrenson, L. K. Schroeder, A. M. Kershner, and G. J. Hermann Role of the Caenorhabditis elegans Multidrug Resistance Gene, mrp-4, in Gut Granule Differentiation Genetics, November 1, 2007; 177(3): 1569 - 1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Berger, G. Salazar, M. L. Styers, K. A. Newell-Litwa, E. Werner, R. A. Maue, A. H. Corbett, and V. Faundez The subcellular localization of the Niemann-Pick Type C proteins depends on the adaptor complex AP-3 J. Cell Sci., October 15, 2007; 120(20): 3640 - 3652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Parker, M. Metzler, J. Georgiou, M. Mage, J. C. Roder, A. M. Rose, M. R. Hayden, and C. Neri Huntingtin-Interacting Protein 1 Influences Worm and Mouse Presynaptic Function and Protects Caenorhabditis elegans Neurons against Mutant Polyglutamine Toxicity J. Neurosci., October 10, 2007; 27(41): 11056 - 11064. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Audhya, A. Desai, and K. Oegema A role for Rab5 in structuring the endoplasmic reticulum J. Cell Biol., October 3, 2007; 178(1): 43 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Willnow, A. Hammes, and S. Eaton Lipoproteins and their receptors in embryonic development: more than cholesterol clearance Development, September 15, 2007; 134(18): 3239 - 3249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Holmes, A. Flett, D. Coudreuse, H. C. Korswagen, and J. Pettitt C. elegans Disabled is required for cell-type specific endocytosis and is essential in animals lacking the AP-3 adaptor complex J. Cell Sci., August 1, 2007; 120(15): 2741 - 2751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Wolke, E. A. Jezuit, and J. R. Priess Actin-dependent cytoplasmic streaming in C. elegans oogenesis Development, June 15, 2007; 134(12): 2227 - 2236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Schaheen, G. Patton, and H. Fares Suppression of the cup-5 mucolipidosis type IV-related lysosomal dysfunction by the inactivation of an ABC transporter in C. elegans Development, October 1, 2006; 133(19): 3939 - 3948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. K. Larsen, S. Tuck, N. J. Faergeman, and J. Knudsen MAA-1, a Novel Acyl-CoA-binding Protein Involved in Endosomal Vesicle Transport in Caenorhabditis elegans Mol. Biol. Cell, October 1, 2006; 17(10): 4318 - 4329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hashmi, J. Zhang, Y. Oksov, Q. Ji, and S. Lustigman The Caenorhabditis elegans CPI-2a Cystatin-like Inhibitor Has an Essential Regulatory Role during Oogenesis and Fertilization J. Biol. Chem., September 22, 2006; 281(38): 28415 - 28429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-i. Ogura and Y. Goshima The autophagy-related kinase UNC-51 and its binding partner UNC-14 regulate the subcellular localization of the Netrin receptor UNC-5 in Caenorhabditis elegans Development, September 1, 2006; 133(17): 3441 - 3450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Chi and V. Reinke Promotion of oogenesis and embryogenesis in the C. elegans gonad by EFL-1/DPL-1 (E2F) does not require LIN-35 (pRB) Development, August 15, 2006; 133(16): 3147 - 3157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hayakawa, D. Leonard, S. Murphy, S. Hayes, M. Soto, K. Fogarty, C. Standley, K. Bellve, D. Lambright, C. Mello, et al. The WD40 and FYVE domain containing protein 2 defines a class of early endosomes necessary for endocytosis PNAS, August 8, 2006; 103(32): 11928 - 11933. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-S. Nicot, H. Fares, B. Payrastre, A. D. Chisholm, M. Labouesse, and J. Laporte The Phosphoinositide Kinase PIKfyve/Fab1p Regulates Terminal Lysosome Maturation in Caenorhabditis elegans Mol. Biol. Cell, July 1, 2006; 17(7): 3062 - 3074. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sato, M. Sato, A. Audhya, K. Oegema, P. Schweinsberg, and B. D. Grant Dynamic Regulation of Caveolin-1 Trafficking in the Germ Line and Embryo of Caenorhabditis elegans Mol. Biol. Cell, July 1, 2006; 17(7): 3085 - 3094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Liegeois, A. Benedetto, J.-M. Garnier, Y. Schwab, and M. Labouesse The V0-ATPase mediates apical secretion of exosomes containing Hedgehog-related proteins in Caenorhabditis elegans J. Cell Biol., June 19, 2006; 173(6): 949 - 961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ohno Physiological Roles of Clathrin Adaptor AP Complexes: Lessons from Mutant Animals J. Biochem., June 1, 2006; 139(6): 943 - 948. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C.-H. Chen, P. J. Schweinsberg, S. Vashist, D. P. Mareiniss, E. J. Lambie, and B. D. Grant RAB-10 Is Required for Endocytic Recycling in the Caenorhabditis elegans Intestine Mol. Biol. Cell, March 1, 2006; 17(3): 1286 - 1297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. K. M. Choy, J. M. Kemner, and J. H. Thomas Fluoxetine-Resistance Genes in Caenorhabditis elegans Function in the Intestine and May Act in Drug Transport Genetics, February 1, 2006; 172(2): 885 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. R. Boag, A. Nakamura, and T. K. Blackwell A conserved RNA-protein complex component involved in physiological germline apoptosis regulation in C. elegans Development, November 15, 2005; 132(22): 4975 - 4986. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Mitsunari, F. Nakatsu, N. Shioda, P. E. Love, A. Grinberg, J. S. Bonifacino, and H. Ohno Clathrin Adaptor AP-2 Is Essential for Early Embryonal Development Mol. Cell. Biol., November 1, 2005; 25(21): 9318 - 9323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Zugasti, J. Rajan, and P. E. Kuwabara The function and expansion of the Patched- and Hedgehog-related homologs in C. elegans Genome Res., October 1, 2005; 15(10): 1402 - 1410. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Bachorik and J. Kimble Redundant control of the Caenorhabditis elegans sperm/oocyte switch by PUF-8 and FBF-1, two distinct PUF RNA-binding proteins PNAS, August 2, 2005; 102(31): 10893 - 10897. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kosinski, K. McDonald, J. Schwartz, I. Yamamoto, and D. Greenstein C. elegans sperm bud vesicles to deliver a meiotic maturation signal to distant oocytes Development, August 1, 2005; 132(15): 3357 - 3369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. E. Thompson, D. S. Bernstein, J. L. Bachorik, A. G. Petcherski, M. Wickens, and J. Kimble Dose-dependent control of proliferation and sperm specification by FOG-1/CPEB Development, August 1, 2005; 132(15): 3471 - 3481. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. E. Vought, M. Ohmachi, M.-H. Lee, and E. M. Maine EGO-1, a Putative RNA-Directed RNA Polymerase, Promotes Germline Proliferation in Parallel With GLP-1/Notch Signaling and Regulates the Spatial Organization of Nuclear Pore Complexes and Germline P Granules in Caenorhabditis elegans Genetics, July 1, 2005; 170(3): 1121 - 1132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Hermann, L. K. Schroeder, C. A. Hieb, A. M. Kershner, B. M. Rabbitts, P. Fonarev, B. D. Grant, and J. R. Priess Genetic Analysis of Lysosomal Trafficking in Caenorhabditis elegans Mol. Biol. Cell, July 1, 2005; 16(7): 3273 - 3288. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Novillo, S.-J. Won, C. Li, and I. P. Callard Changes in Nuclear Receptor and Vitellogenin Gene Expression in Response to Steroids and Heavy Metal in Caenorhabditis elegans Integr. Comp. Biol., January 1, 2005; 45(1): 61 - 71. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Haupt, G. H. Cowan, A. Ziegler, A. G. Roberts, K. J. Oparka, and L. Torrance Two Plant-Viral Movement Proteins Traffic in the Endocytic Recycling Pathway PLANT CELL, January 1, 2005; 17(1): 164 - 181. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li, G. Brown, M. Ailion, S. Lee, and J. H. Thomas NCR-1 and NCR-2, the C. elegans homologs of the human Niemann-Pick type C1 disease protein, function upstream of DAF-9 in the dauer formation pathways Development, November 15, 2004; 131(22): 5741 - 5752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Moribe, J. Yochem, H. Yamada, Y. Tabuse, T. Fujimoto, and E. Mekada Tetraspanin protein (TSP-15) is required for epidermal integrity in Caenorhabditis elegans J. Cell Sci., October 15, 2004; 117(22): 5209 - 5220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Britton and L. Murray Cathepsin L protease (CPL-1) is essential for yolk processing during embryogenesis in Caenorhabditis elegans J. Cell Sci., October 1, 2004; 117(21): 5133 - 5143. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Ciosk, M. DePalma, and J. R. Priess ATX-2, the C. elegans ortholog of ataxin 2, functions in translational regulation in the germline Development, October 1, 2004; 131(19): 4831 - 4841. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Brooks and G. M. Wessel The Major Yolk Protein of Sea Urchins Is Endocytosed by a Dynamin-Dependent Mechanism Biol Reprod, September 1, 2004; 71(3): 705 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Meissner, M. Boll, H. Daniel, and R. Baumeister Deletion of the Intestinal Peptide Transporter Affects Insulin and TOR Signaling in Caenorhabditis elegans J. Biol. Chem., August 27, 2004; 279(35): 36739 - 36745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ono and S. Ono Tropomyosin and Troponin Are Required for Ovarian Contraction in the Caenorhabditis elegans Reproductive System Mol. Biol. Cell, June 1, 2004; 15(6): 2782 - 2793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-H. Lee and T. Schedl Translation repression by GLD-1 protects its mRNA targets from nonsense-mediated mRNA decay in C. elegans Genes & Dev., May 1, 2004; 18(9): 1047 - 1059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shibata, R. Branicky, I. O. Landaverde, and S. Hekimi Redox Regulation of Germline and Vulval Development in Caenorhabditis elegans Science, December 5, 2003; 302(5651): 1779 - 1782. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Kamikura and J. A. Cooper Lipoprotein receptors and a Disabled family cytoplasmic adaptor protein regulate EGL-17/FGF export in C. elegans Genes & Dev., November 15, 2003; 17(22): 2798 - 2811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhou, J. Zhang, and M. L. King Xenopus Autosomal Recessive Hypercholesterolemia Protein Couples Lipoprotein Receptors with the AP-2 Complex in Oocytes and Embryos and Is Required for Vitellogenesis J. Biol. Chem., November 7, 2003; 278(45): 44584 - 44592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Roberts, C. Clucas, and I. L. Johnstone Loss of SEC-23 in Caenorhabditis elegans Causes Defects in Oogenesis, Morphogenesis, and Extracellular Matrix Secretion Mol. Biol. Cell, November 1, 2003; 14(11): 4414 - 4426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.-K. Choi, D. J. Chitwood, and Y.-K. Paik Proteomic Changes during Disturbance of Cholesterol Metabolism by Azacoprostane Treatment in Caenorhabditis elegans Mol. Cell. Proteomics, October 1, 2003; 2(10): 1086 - 1095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-c. Huang, D. H. Hall, E. M. Hedgecock, G. Kao, V. Karantza, B. E. Vogel, H. Hutter, A. D. Chisholm, P. D. Yurchenco, and W. G. Wadsworth Laminin {alpha} subunits and their role in C. elegans development Development, July 15, 2003; 130(14): 3343 - 3358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wucherpfennig, M. Wilsch-Brauninger, and M. Gonzalez-Gaitan Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter release J. Cell Biol., May 12, 2003; 161(3): 609 - 624. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Strange From Genes to Integrative Physiology: Ion Channel and Transporter Biology in Caenorhabditis elegans Physiol Rev, April 1, 2003; 83(2): 377 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Miller, P. J. Ruest, M. Kosinski, S. K. Hanks, and D. Greenstein An Eph receptor sperm-sensing control mechanism for oocyte meiotic maturation in Caenorhabditis elegans Genes & Dev., January 15, 2003; 17(2): 187 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hashmi, C. Britton, J. Liu, D. B. Guiliano, Y. Oksov, and S. Lustigman Cathepsin L Is Essential for Embryogenesis and Development of Caenorhabditis elegans J. Biol. Chem., January 25, 2002; 277(5): 3477 - 3486. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Basham and L. S. Rose The Caenorhabditis elegans polarity gene ooc-5 encodes a Torsin-related protein of the AAA ATPase superfamily Development, November 15, 2001; 128(22): 4645 - 4656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Boehm and J. S. Bonifacino Adaptins. The Final Recount Mol. Biol. Cell, October 1, 2001; 12(10): 2907 - 2920. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-H. Lee and T. Schedl Identification of in vivo mRNA targets of GLD-1, a maxi-KH motif containing protein required for C. elegans germ cell development Genes & Dev., September 15, 2001; 15(18): 2408 - 2420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Fares and I. Greenwald Genetic Analysis of Endocytosis in Caenorhabditis elegans: Coelomocyte Uptake Defective Mutants Genetics, September 1, 2001; 159(1): 133 - 145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Navarro, E. Y. Shim, Y. Kohara, A. Singson, and T. K. Blackwell cgh-1, a conserved predicted RNA helicase required for gametogenesis and protection from physiological germline apoptosis in C. elegans Development, September 1, 2001; 128(17): 3221 - 3232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-C. Paupard, A. Miller, B. Grant, D. Hirsh, and D. H. Hall Immuno-EM Localization of GFP-tagged Yolk Proteins in C. elegans Using Microwave Fixation J. Histochem. Cytochem., August 1, 2001; 49(8): 949 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, B. Grant, and D. Hirsh RME-8, a Conserved J-Domain Protein, Is Required for Endocytosis in Caenorhabditis elegans Mol. Biol. Cell, July 1, 2001; 12(7): 2011 - 2021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Matyash, C. Geier, A. Henske, S. Mukherjee, D. Hirsh, C. Thiele, B. Grant, F. R. Maxfield, and T. V. Kurzchalia Distribution and Transport of Cholesterol in Caenorhabditis elegans Mol. Biol. Cell, June 1, 2001; 12(6): 1725 - 1736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Hong, T. Elbl, J. Ward, C. Franzini-Armstrong, K. K. Rybicka, B. K. Gatewood, D. L. Baillie, and E. A. Bucher MUP-4 is a novel transmembrane protein with functions in epithelial cell adhesion in Caenorhabditis elegans J. Cell Biol., July 23, 2001; 154(2): 403 - 414. [Abstract] [Full Text] [PDF]
|
|
