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Vol. 10, Issue 12, 4429-4439, December 1999
*Department of Cell Biology, Duke University Medical Center,
Durham, North Carolina 27710; and #Section of Molecular
Cell and Developmental Biology, University of Texas at Austin, Austin,
Texas 78712
We isolated a Dictyostelium cytokinesis mutant with
a defect in a novel locus called large volume sphere A
(lvsA). lvsA mutants exhibit an unusual
phenotype when attempting to undergo cytokinesis in suspension culture.
Early in cytokinesis, they initiate furrow formation with concomitant
myosin II localization at the cleavage furrow. However, the furrow is
later disrupted by a bulge that forms in the middle of the cell. This
bulge is bounded by furrows on both sides, which are often enriched in
myosin II. The bulge can increase and decrease in size multiple times
as the cell attempts to divide. Interestingly, this phenotype is
similar to the cytokinesis failure of Dictyostelium
clathrin heavy-chain mutants. Furthermore, both cell lines cap ConA
receptors but form only a C-shaped loose cap. Unlike clathrin mutants,
lvsA mutants are not defective in endocytosis or
development. The LvsA protein shares several domains in common with the
molecules beige and Chediak-Higashi syndrome proteins that are
important for lysosomal membrane traffic. Thus, on the basis of the
sequence analysis of the LvsA protein and the phenotype of the
lvsA mutants, we postulate that LvsA plays an important
role in a membrane-processing pathway that is essential for cytokinesis.
Online version of this article contains video material
for Figure 4. Online version available at www.molbiolcell.org.
¶
Corresponding author: 241 Patterson Building, Mail
code C0930, Section of Molecular Cell and Developmental Biology,
University of Texas at Austin, Austin, TX 78712. E-mail:
a.delozanne{at}mail.utexas.edu.
Present addresses: Department of Biology,
Clark University, 950 Main Street, Worcester, MA 01610;
Department of Molecular Biology, Cleveland Clinic
Research Institute, 9500 Euclid Avenue, Cleveland, OH, 44195;
§
Antigenics, L.L.C., 34 Commerce Way, Woburn, MA 01801.
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