![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 2, 283-296, February 1999
Department of Cell Biology, Harvard Medical School, Boston,
Massachusetts 02115
The budding yeast IQGAP-like protein Cyk1p/Iqg1p localizes
to the mother-bud junction during anaphase and has been shown to be
required for the completion of cytokinesis. In this study, video
microscopy analysis of cells expressing green fluorescent protein-tagged Cyk1p/Iqg1p demonstrates that Cyk1p/Iqg1p is a dynamic component of the contractile ring during cytokinesis. Furthermore, in the absence of Cyk1p/Iqg1p, myosin II fails to undergo
the contraction-like size change at the end of mitosis. To understand
the mechanistic role of Cyk1p/Iqg1p in actomyosin ring assembly and
dynamics, we have investigated the role of the structural domains that
Cyk1p/Iqg1p shares with IQGAPs. An amino terminal portion
containing the calponin homology domain binds to actin filaments and is
required for the assembly of actin filaments to the ring. This result
supports the hypothesis that Cyk1p/Iqg1p plays a direct role in F-actin
recruitment. Deletion of the domain harboring the eight IQ motifs
abolishes the localization of Cyk1p/Iqg1p to the bud neck, suggesting
that Cyk1p/Iqg1p may be localized through interactions with a
calmodulin-like protein. Interestingly, deletion of the COOH-terminal
GTPase-activating protein-related domain does not affect Cyk1p/Iqg1p
localization or actin recruitment to the ring but prevents actomyosin
ring contraction. In vitro binding experiments show that Cyk1p/Iqg1p
binds to calmodulin, Cmd1p, in a calcium-dependent manner, and to
Tem1p, a small GTP-binding protein previously found to be required for
the completion of anaphase. These results demonstrate the critical
function of Cyk1p/Iqg1p in regulating various steps of actomyosin ring
assembly and cytokinesis.
This article has been cited by other articles:
|
|
 |
|
  D. J. Wright, E. Munro, M. Corbett, A. J. Bentley, N. J. Fullwood, S. Murray, and C. Price The Saccharomyces cerevisiae Actin Cytoskeletal Component Bsp1p Has an Auxiliary Role in Actomyosin Ring Function and in the Maintenance of Bud-Neck Structure Genetics, April 1, 2008; 178(4): 1903 - 1914. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ko, R. Nishihama, G. H. Tully, D. Ostapenko, M. J. Solomon, D. O. Morgan, and J. R. Pringle Identification of Yeast IQGAP (Iqg1p) as an Anaphase-Promoting-Complex Substrate and Its Role in Actomyosin-Ring-Independent Cytokinesis Mol. Biol. Cell, December 1, 2007; 18(12): 5139 - 5153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Le Clainche, D. Schlaepfer, A. Ferrari, M. Klingauf, K. Grohmanova, A. Veligodskiy, D. Didry, D. Le, C. Egile, M.-F. Carlier, et al. IQGAP1 Stimulates Actin Assembly through the N-Wasp-Arp2/3 Pathway J. Biol. Chem., January 5, 2007; 282(1): 426 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Moseley and B. L. Goode The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism Microbiol. Mol. Biol. Rev., September 1, 2006; 70(3): 605 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hwa Lim, F. M. Yeong, and U. Surana Inactivation of Mitotic Kinase Triggers Translocation of MEN Components to Mother-Daughter Neck in Yeast Mol. Biol. Cell, November 1, 2003; 14(11): 4734 - 4743. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Osman, J. B. Konopka, and R. A. Cerione Iqg1p links spatial and secretion landmarks to polarity and cytokinesis J. Cell Biol., November 25, 2002; 159(4): 601 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Guertin, S. Trautmann, and D. McCollum Cytokinesis in Eukaryotes Microbiol. Mol. Biol. Rev., June 1, 2002; 66(2): 155 - 178. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Richman, M. M. Sawyer, and D. I. Johnson Saccharomyces cerevisiae Cdc42p Localizes to Cellular Membranes and Clusters at Sites of Polarized Growth Eukaryot. Cell, June 1, 2002; 1(3): 458 - 468. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. N. Robinson, S. S. Ocon, R. S. Rock, and J. A. Spudich Dynacortin Is a Novel Actin Bundling Protein That Localizes to Dynamic Actin Structures J. Biol. Chem., March 8, 2002; 277(11): 9088 - 9095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Lippincott, K. Shannon, W Shou, R. Deshaies, and R Li The Tem1 small GTPase controls actomyosin and septin dynamics during cytokinesis J. Cell Sci., January 4, 2001; 114(7): 1379 - 1386. [Abstract] [PDF]
|
|
|
|
 |
|
 H.-U. Mösch, T. Köhler, and G. H. Braus Different Domains of the Essential GTPase Cdc42p Required for Growth and Development of Saccharomyces cerevisiae Mol. Cell. Biol., January 1, 2001; 21(1): 235 - 248. [Abstract] [Full Text]
|
|
|
|
|
|
E. Bi, J. B. Chiavetta, H. Chen, G.-C. Chen, C. S. M. Chan, and J. R. Pringle Identification of Novel, Evolutionarily Conserved Cdc42p-interacting Proteins and of Redundant Pathways Linking Cdc24p and Cdc42p to Actin Polarization in Yeast Mol. Biol. Cell, February 1, 2000; 11(2): 773 - 793. [Abstract] [Full Text]
|
|
|
|
|
|
S. Li, Q. Wang, A. Chakladar, R. T. Bronson, and A. Bernards Gastric Hyperplasia in Mice Lacking the Putative Cdc42 Effector IQGAP1 Mol. Cell. Biol., January 15, 2000; 20(2): 697 - 701. [Abstract] [Full Text]
|
|
|
|
|
|
J. Boyne, H. Yosuf, P Bieganowski, C Brenner, and C Price Yeast myosin light chain, Mlc1p, interacts with both IQGAP and class II myosin to effect cytokinesis J. Cell Sci., January 12, 2000; 113(24): 4533 - 4543. [Abstract] [PDF]
|
|
|
|
|
|
L. Frenz, S. Lee, D Fesquet, and L. Johnston The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis J. Cell Sci., January 10, 2000; 113(19): 3399 - 3408. [Abstract] [PDF]
|
|
|
|
|
|
M. Balasubramanian, D McCollum, and U Surana Tying the knot: linking cytokinesis to the nuclear cycle J. Cell Sci., January 5, 2000; 113(9): 1503 - 1513. [Abstract] [PDF]
|
|
|
|
|
|
F Motegi, K Nakano, and I Mabuchi Molecular mechanism of myosin-II assembly at the division site in Schizosaccharomyces pombe J. Cell Sci., January 5, 2000; 113(10): 1813 - 1825. [Abstract] [PDF]
|
|
|
|
|
|
S. P. Holly and K. J. Blumer PAK-Family Kinases Regulate Cell and Actin Polarization throughout the Cell Cycle of Saccharomyces cerevisiae J. Cell Biol., November 15, 1999; 147(4): 845 - 856. [Abstract] [Full Text] [PDF]
|
|
