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Vol. 10, Issue 2, 297-311, February 1999
and§
*Department of Biomedical Sciences, State University of New York,
Albany, New York 12222;
PtK1 cells containing two independent mitotic
spindles can cleave between neighboring centrosomes, in the absence of
an intervening spindle, as well as at the spindle equators. We
used same-cell video, immunofluorescence, and electron microscopy to
compare the structure and composition of normal equatorial furrows with that of ectopic furrows formed between spindles. As in controls, ectopic furrows contained midbodies composed of microtubule bundles and
an electron-opaque matrix. Despite the absence of an intervening spindle and chromosomes, the midbodies associated with ectopic furrows
also contained the microtubule-bundling protein CHO1 and the
chromosomal passenger protein INCENP. However, CENP-E,
another passenger protein, was not found in ectopic furrows but was
always present in controls. We also examined cells in which the ectopic furrow initiated but relaxed. Although relaxing furrows contained overlapping microtubules from opposing centrosomes, they lacked microtubule bundles as well as INCENP and CHO1. Together these data
suggest that the mechanism defining the site of furrow formation during mitosis in vertebrates does not depend on the presence of
underlying microtubule bundles and chromosomes or on the stable association of INCENP or CHO1. The data also suggest that the completion of cytokinesis requires the presence of microtubule bundles
and specific proteins (e.g., INCENP, CHO1, etc.) that do not include
CENP-E.
Division of Molecular Medicine,
Wadsworth Center, New York State Department of Health, Albany, New York
12201-0509; and
Institute of Cell and Molecular Biology,
University at Edinburgh, Edinburgh EH9 3JR, Scotland, United
Kingdom
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