![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 2, 435-453, February 1999
Membrane Biology Laboratory, Institute of Molecular and Cell
Biology, Singapore 117609, Singapore
Yeast Sec22p participates in both anterograde and retrograde
vesicular transport between the endoplasmic reticulum (ER) and the
Golgi apparatus by functioning as a v-SNARE (soluble
N-ethylmaleimide-sensitive factor [NSF] attachment
protein receptor) of transport vesicles. Three mammalian proteins
homologous to Sec22p have been identified and are referred to as
Sec22a, Sec22b/ERS-24, and Sec22c, respectively. The existence of three
homologous proteins in mammalian cells calls for detailed cell
biological and functional examinations of each individual protein. The
epitope-tagged forms of all three proteins have been shown to be
primarily associated with the ER, although functional examination has
not been carefully performed for any one of them. In this study, using
antibodies specific for Sec22b/ERS-24, it is revealed that endogenous
Sec22b/ERS-24 is associated with vesicular structures in both the
perinuclear Golgi and peripheral regions. Colabeling experiments for
Sec22b/ERS-24 with Golgi mannosidase II, the KDEL receptor, and
the envelope glycoprotein G (VSVG) of vesicular stomatitis virus (VSV)
en route from the ER to the Golgi under normal, brefeldin A, or
nocodazole-treated cells suggest that Sec22b/ERS-24 is enriched in the
pre-Golgi intermediate compartment (IC). In a well-established
semi-intact cell system that reconstitutes transport from the ER to the
Golgi, transport of VSVG is inhibited by antibodies against
Sec22b/ERS-24. EGTA is known to inhibit ER-Golgi transport at a stage
after vesicle/transport intermediate docking but before the actual
fusion event. Antibodies against Sec22b/ERS-24 inhibit ER-Golgi
transport only when they are added before the EGTA-sensitive stage.
Transport of VSVG accumulated in pre-Golgi IC by incubation at 15°C
is also inhibited by Sec22b/ERS-24 antibodies. Morphologically, VSVG is
transported from the ER to the Golgi apparatus via vesicular
intermediates that scatter in the peripheral as well as the Golgi
regions. In the presence of antibodies against Sec22b/ERS-24, VSVG is
seen to accumulate in these intermediates, suggesting that
Sec22b/ERS-24 functions at the level of the IC in ER-Golgi transport.
This article has been cited by other articles:
|
|
 |
|
  D. Williams and J. E. Pessin Mapping of R-SNARE function at distinct intracellular GLUT4 trafficking steps in adipocytes J. Cell Biol., January 28, 2008; 180(2): 375 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Saraste and B. Goud Functional Symmetry of Endomembranes Mol. Biol. Cell, April 1, 2007; 18(4): 1430 - 1436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. T. Tran, Q. Zeng, and W. Hong VAMP4 cycles from the cell surface to the trans-Golgi network via sorting and recycling endosomes J. Cell Sci., March 15, 2007; 120(6): 1028 - 1041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hatsuzawa, T. Tamura, H. Hashimoto, H. Hashimoto, S. Yokoya, M. Miura, H. Nagaya, and I. Wada Involvement of Syntaxin 18, an Endoplasmic Reticulum (ER)-localized SNARE Protein, in ER-mediated Phagocytosis Mol. Biol. Cell, September 1, 2006; 17(9): 3964 - 3977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Okumura, K. Hatsuzawa, T. Tamura, H. Nagaya, K. Saeki, F. Okumura, K. Nagao, M. Nishikawa, A. Yoshimura, and I. Wada Involvement of a Novel Q-SNARE, D12, in Quality Control of the Endomembrane System J. Biol. Chem., February 17, 2006; 281(7): 4495 - 4506. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Loh, F. Peter, V. N. Subramaniam, and W. Hong Mammalian Bet3 functions as a cytosolic factor participating in transport from the ER to the Golgi apparatus J. Cell Sci., March 15, 2005; 118(6): 1209 - 1222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Breuza, R. Halbeisen, P. Jeno, S. Otte, C. Barlowe, W. Hong, and H.-P. Hauri Proteomics of Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC) Membranes from Brefeldin A-treated HepG2 Cells Identifies ERGIC-32, a New Cycling Protein That Interacts with Human Erv46 J. Biol. Chem., November 5, 2004; 279(45): 47242 - 47253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Tai, L. Lu, T. L. Wang, B. L. Tang, B. Goud, L. Johannes, and W. Hong Participation of the Syntaxin 5/Ykt6/GS28/GS15 SNARE Complex in Transport from the Early/Recycling Endosome to the Trans-Golgi Network Mol. Biol. Cell, September 1, 2004; 15(9): 4011 - 4022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. Kagan, M.-P. Stein, M. Pypaert, and C. R. Roy Legionella Subvert the Functions of Rab1 and Sec22b to Create a Replicative Organelle J. Exp. Med., May 3, 2004; 199(9): 1201 - 1211. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Volchuk, M. Ravazzola, A. Perrelet, W. S. Eng, M. Di Liberto, O. Varlamov, M. Fukasawa, T. Engel, T. H. Sollner, J. E. Rothman, et al. Countercurrent Distribution of Two Distinct SNARE Complexes Mediating Transport within the Golgi Stack Mol. Biol. Cell, April 1, 2004; 15(4): 1506 - 1518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Horstmann, C. P. Ng, B. L. Tang, and W. Hong Ultrastructural characterization of endoplasmic reticulum -- Golgi transport containers (EGTC) J. Cell Sci., November 15, 2002; 115(22): 4263 - 4273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, S. Martin, D. E. James, and W. Hong GS15 Forms a SNARE Complex with Syntaxin 5, GS28, and Ykt6 and Is Implicated in Traffic in the Early Cisternae of the Golgi Apparatus Mol. Biol. Cell, October 1, 2002; 13(10): 3493 - 3507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu and C. Barlowe Analysis of Sec22p in Endoplasmic Reticulum/Golgi Transport Reveals Cellular Redundancy in SNARE Protein Function Mol. Biol. Cell, September 1, 2002; 13(9): 3314 - 3324. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Govers, P. van der Sluijs, E. van Donselaar, J.-W. Slot, and T. J. Rabelink Endothelial Nitric Oxide Synthase and Its Negative Regulator Caveolin-1 Localize to Distinct Perinuclear Organelles J. Histochem. Cytochem., June 1, 2002; 50(6): 779 - 788. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Parlati, O. Varlamov, K. Paz, J. A. McNew, D. Hurtado, T. H. Sollner, and J. E. Rothman Distinct SNARE complexes mediating membrane fusion in Golgi transport based on combinatorial specificity PNAS, April 16, 2002; 99(8): 5424 - 5429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. T. Hammond and B. S. Glick Dynamics of Transitional Endoplasmic Reticulum Sites in Vertebrate Cells Mol. Biol. Cell, September 1, 2000; 11(9): 3013 - 3030. [Abstract] [Full Text]
|
|
|
|
|
|
B. L. Tang, T. Zhang, D. Y. H. Low, E. T. Wong, H. Horstmann, and W. Hong Mammalian Homologues of Yeast Sec31p. AN UBIQUITOUSLY EXPRESSED FORM IS LOCALIZED TO ENDOPLASMIC RETICULUM (ER) EXIT SITES AND IS ESSENTIAL FOR ER-GOLGI TRANSPORT J. Biol. Chem., April 28, 2000; 275(18): 13597 - 13604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Bell, M. A. Ward, W. P. Blackstock, H. N. M. Freeman, J. S. Choudhary, A. P. Lewis, D. Chotai, A. Fazel, J. N. Gushue, J. Paiement, et al. Proteomics Characterization of Abundant Golgi Membrane Proteins J. Biol. Chem., February 9, 2001; 276(7): 5152 - 5165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Xu, A. P. Joglekar, A. L. Williams, and J. C. Hay Subunit Structure of a Mammalian ER/Golgi SNARE Complex J. Biol. Chem., December 8, 2000; 275(50): 39631 - 39639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Alvarez, R. Garcia-Mata, H.-P. Hauri, and E. Sztul The p115-interactive Proteins GM130 and Giantin Participate in Endoplasmic Reticulum-Golgi Traffic J. Biol. Chem., January 19, 2001; 276(4): 2693 - 2700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Zhang and W. Hong Ykt6 Forms a SNARE Complex with Syntaxin 5, GS28, and Bet1 and Participates in a Late Stage in Endoplasmic Reticulum-Golgi Transport J. Biol. Chem., July 13, 2001; 276(29): 27480 - 27487. [Abstract] [Full Text] [PDF]
|
|
