![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 2, 487-499, February 1999
and
*Department of Embryology, Carnegie Institution, Baltimore,
Maryland 21210; and
The stem-loop binding protein (SLBP1) binds the 3' stem-loop
of histone pre-mRNA and is required for efficient processing of histone
transcripts in the nucleus. We examined the localization of SLBP1 in
the germinal vesicle of Xenopus laevis oocytes.
In spread preparations of germinal vesicle contents, an anti-SLBP1 antibody stained coiled bodies and specific chromosomal loci, including
terminal granules, axial granules, and some loops. After injection of
myc-tagged SLBP1 transcripts into the oocyte cytoplasm, newly translated myc-SLBP1 protein was detectable in
coiled bodies within 4 h and in terminal and axial granules by
8 h. To identify the region(s) of SLBP1 necessary for subnuclear
localization, we subcloned various parts of the SLBP1 cDNA and injected
transcripts of these into the cytoplasm of oocytes. We determined that
113 amino acids at the carboxy terminus of SLBP1 are sufficient for coiled body localization and that disruption of a previously defined RNA-binding domain did not alter this localization. Coiled bodies also
contain the U7 small nuclear ribonucleoprotein particle (snRNP), which participates in cleavage of the 3' end of histone pre-mRNA. The
colocalization of SLBP1 and the U7 snRNP in the coiled body suggests coordinated control of their functions, perhaps through a
larger histone-processing particle. Some coiled bodies are attached to
the lampbrush chromosomes at the histone gene loci, consistent with the
view that coiled bodies in the oocyte recruit histone-processing factors to the sites of histone pre-mRNA transcription. The non-histone chromosomal sites at which SLBP1 is found include the genes coding for
5 S rRNA, U1 snRNA, and U2 snRNA, suggesting a wider role for SLBP1 in
the biosynthesis of small non-spliced RNAs.
Department of Biochemistry and
Biophysics, University of North Carolina, Chapel Hill, North Carolina,
27599
Corresponding author: Department of
Embryology, Carnegie Institution, 115 West University Parkway,
Baltimore, MD 21210. E-mail address: gall{at}mail1.ciwemb.edu.
This article has been cited by other articles:
|
|
 |
|
  E. J. Wagner, J. K. Ospina, Y. Hu, M. Dundr, A. G. Matera, and W. F. Marzluff Conserved zinc fingers mediate multiple functions of ZFP100, a U7snRNP associated protein RNA, July 1, 2006; 12(7): 1206 - 1218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. GODFREY, J. M. KUPSCO, B. D. BURCH, R. M. ZIMMERMAN, Z. DOMINSKI, W. F. MARZLUFF, and R. J. DURONIO U7 snRNA mutations in Drosophila block histone pre-mRNA processing and disrupt oogenesis. RNA, March 1, 2006; 12(3): 396 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. G. Kolev and J. A. Steitz Symplekin and multiple other polyadenylation factors participate in 3'-end maturation of histone mRNAs Genes & Dev., November 1, 2005; 19(21): 2583 - 2592. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Erkmann, E. J. Wagner, J. Dong, Y. Zhang, U. Kutay, and W. F. Marzluff Nuclear Import of the Stem-Loop Binding Protein and Localization during the Cell Cycle Mol. Biol. Cell, June 1, 2005; 16(6): 2960 - 2971. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Smith, Y. Ling, and G. T. Morgan Subnuclear Localization and Cajal Body Targeting of Transcription Elongation Factor TFIIS in Amphibian Oocytes Mol. Biol. Cell, March 1, 2003; 14(3): 1255 - 1267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Handwerger, Z.'a. Wu, C. Murphy, and J. G. Gall Heat shock induces mini-Cajal bodies in the Xenopus germinal vesicle J. Cell Sci., May 15, 2002; 115(10): 2011 - 2020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Allard, M. J. Champigny, S. Skoggard, J. A. Erkmann, M. L. Whitfield, W. F. Marzluff, and H. J. Clarke Stem-loop binding protein accumulates during oocyte maturation and is not cell-cycle-regulated in the early mouse embryo J. Cell Sci., January 12, 2002; 115(23): 4577 - 4586. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Shopland, M. Byron, J. L. Stein, J. B. Lian, G. S. Stein, and J. B. Lawrence Replication-dependent Histone Gene Expression Is Related to Cajal Body (CB) Association but Does Not Require Sustained CB Contact Mol. Biol. Cell, March 1, 2001; 12(3): 565 - 576. [Abstract] [Full Text]
|
|
|
|
|
|
T. Ma, B. A. Van Tine, Y. Wei, M. D. Garrett, D. Nelson, P. D. Adams, J. Wang, J. Qin, L. T. Chow, and J. W. Harper Cell cycle-regulated phosphorylation of p220NPAT by cyclin E/Cdk2 in Cajal bodies promotes histone gene transcription Genes & Dev., September 15, 2000; 14(18): 2298 - 2313. [Abstract] [Full Text]
|
|
|
|
|
|
K. P. Smith and J. B. Lawrence Interactions of U2 Gene Loci and Their Nuclear Transcripts with Cajal (Coiled) Bodies: Evidence for PreU2 within Cajal Bodies Mol. Biol. Cell, September 1, 2000; 11(9): 2987 - 2998. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. D. Lewis and D. Tollervey Like Attracts Like: Getting RNA Processing Together in the Nucleus Science, May 26, 2000; 288(5470): 1385 - 1389. [Abstract] [Full Text]
|
|
|
|
|
|
J Liu, M. Hebert, Y Ye, D. Templeton, H Kung, and A. Matera Cell cycle-dependent localization of the CDK2-cyclin E complex in Cajal (coiled) bodies J. Cell Sci., January 5, 2000; 113(9): 1543 - 1552. [Abstract] [PDF]
|
|
|
|
|
|
J. G. Gall, M. Bellini, Z.'a. Wu, and C. Murphy Assembly of the Nuclear Transcription and Processing Machinery: Cajal Bodies (Coiled Bodies) and Transcriptosomes Mol. Biol. Cell, December 1, 1999; 10(12): 4385 - 4402. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Carvalho, F. Almeida, A. Calapez, M. Lafarga, M. T. Berciano, and M. Carmo-Fonseca The Spinal Muscular Atrophy Disease Gene Product, SMN: A Link between snRNP Biogenesis and the Cajal (Coiled) Body J. Cell Biol., November 15, 1999; 147(4): 715 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Schul, I. van der Kraan, A. G. Matera, R. van Driel, and L. de Jong Nuclear Domains Enriched in RNA 3'-processing Factors Associate with Coiled Bodies and Histone Genes in a Cell Cycle-dependent Manner Mol. Biol. Cell, November 1, 1999; 10(11): 3815 - 3824. [Abstract] [Full Text]
|
|
|
|
|
|
M. Bellini and J. G. Gall Coilin Shuttles between the Nucleus and Cytoplasm In Xenopus Oocytes Mol. Biol. Cell, October 1, 1999; 10(10): 3425 - 3434. [Abstract] [Full Text]
|
|
|
|
 |
|
 Z. Dominski, L.-X. Zheng, R. Sanchez, and W. F. Marzluff Stem-Loop Binding Protein Facilitates 3'-End Formation by Stabilizing U7 snRNP Binding to Histone Pre-mRNA Mol. Cell. Biol., May 1, 1999; 19(5): 3561 - 3570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Muller, J. Link, and C. Smythe Assembly of U7 Small Nuclear Ribonucleoprotein Particle and Histone RNA 3' Processing in Xenopus Egg Extracts J. Biol. Chem., August 4, 2000; 275(32): 24284 - 24293. [Abstract] [Full Text] [PDF]
|
|
