![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 3, 693-712, March 1999
and
*Department of Cell Biology and Neuroanatomy, University of
Minnesota Medical School, Minneapolis, Minnesota 55455; and
A second cytoplasmic dynein heavy chain (cDhc) has recently been
identified in several organisms, and its expression pattern is
consistent with a possible role in axoneme assembly. We have used a genetic approach to ask whether cDhc1b is involved in flagellar assembly in Chlamydomonas. Using a modified PCR
protocol, we recovered two cDhc sequences distinct from the axonemal
Dhc sequences identified previously. cDhc1a is closely related to the
major cytoplasmic Dhc, whereas cDhc1b is closely related to the minor
cDhc isoform identified in sea urchins, Caenorhabditis
elegans, and Tetrahymena. The
Chlamydomonas cDhc1b transcript is a low-abundance mRNA
whose expression is enhanced by deflagellation. To determine its role in flagellar assembly, we screened a collection of stumpy flagellar (stf) mutants generated by insertional mutagenesis and
identified two strains in which portions of the cDhc1b
gene have been deleted. The two mutants assemble short flagellar stumps
(<1-2 µm) filled with aberrant microtubules, raft-like particles,
and other amorphous material. The results indicate that cDhc1b is
involved in the transport of components required for flagellar
assembly in Chlamydomonas.
Department of Molecular Biosciences, University of
Kansas, Lawrence, Kansas 66045
Corresponding author: 4-102 Owre
Hall, Department of Cell Biology and Neuroanatomy, 321 Church Street
Southeast, Minneapolis, MN 55455. E-mail address:
mary-p{at}biosci.cbs.umn.edu.
This article has been cited by other articles:
|
|
 |
|
  C. L. Williams, M. E. Winkelbauer, J. C. Schafer, E. J. Michaud, and B. K. Yoder Functional Redundancy of the B9 Proteins and Nephrocystins in Caenorhabditis elegans Ciliogenesis Mol. Biol. Cell, May 1, 2008; 19(5): 2154 - 2168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Absalon, T. Blisnick, L. Kohl, G. Toutirais, G. Dore, D. Julkowska, A. Tavenet, and P. Bastin Intraflagellar Transport and Functional Analysis of Genes Required for Flagellum Formation in Trypanosomes Mol. Biol. Cell, March 1, 2008; 19(3): 929 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-C. Tsao and M. A. Gorovsky Tetrahymena IFT122A is not essential for cilia assembly but plays a role in returning IFT proteins from the ciliary tip to the cell body J. Cell Sci., February 15, 2008; 121(4): 428 - 436. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Scholey Intraflagellar transport motors in cilia: moving along the cell's antenna J. Cell Biol., January 10, 2008; 180(1): 23 - 29. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Rompolas, L. B. Pedersen, R. S. Patel-King, and S. M. King Chlamydomonas FAP133 is a dynein intermediate chain associated with the retrograde intraflagellar transport motor J. Cell Sci., October 15, 2007; 120(20): 3653 - 3665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ou, M. Koga, O. E. Blacque, T. Murayama, Y. Ohshima, J. C. Schafer, C. Li, B. K. Yoder, M. R. Leroux, and J. M. Scholey Sensory Ciliogenesis in Caenorhabditis elegans: Assignment of IFT Components into Distinct Modules Based on Transport and Phenotypic Profiles Mol. Biol. Cell, May 1, 2007; 18(5): 1554 - 1569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hou, H. Qin, J. A. Follit, G. J. Pazour, J. L. Rosenbaum, and G. B. Witman Functional analysis of an individual IFT protein: IFT46 is required for transport of outer dynein arms into flagella J. Cell Biol., February 26, 2007; 176(5): 653 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. E. Blacque, C. Li, P. N. Inglis, M. A. Esmail, G. Ou, A. K. Mah, D. L. Baillie, J. M. Scholey, and M. R. Leroux The WD Repeat-containing Protein IFTA-1 Is Required for Retrograde Intraflagellar Transport Mol. Biol. Cell, December 1, 2006; 17(12): 5053 - 5062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Hook and R. B. Vallee The dynein family at a glance J. Cell Sci., November 1, 2006; 119(21): 4369 - 4371. [Full Text] [PDF]
|
|
|
|
|
|
E. Efimenko, O. E. Blacque, G. Ou, C. J. Haycraft, B. K. Yoder, J. M. Scholey, M. R. Leroux, and P. Swoboda Caenorhabditis elegans DYF-2, an Orthologue of Human WDR19, Is a Component of the Intraflagellar Transport Machinery in Sensory Cilia Mol. Biol. Cell, November 1, 2006; 17(11): 4801 - 4811. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Davenport and B. K. Yoder An incredible decade for the primary cilium: a look at a once-forgotten organelle Am J Physiol Renal Physiol, December 1, 2005; 289(6): F1159 - F1169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Shapiro, J. Ingram, and K. A. Johnson Characterization of a Molecular Chaperone Present in the Eukaryotic Flagellum Eukaryot. Cell, September 1, 2005; 4(9): 1591 - 1594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Dentler Intraflagellar transport (IFT) during assembly and disassembly of Chlamydomonas flagella J. Cell Biol., August 15, 2005; 170(4): 649 - 659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Huangfu and K. V. Anderson From the Cover: Cilia and Hedgehog responsiveness in the mouse PNAS, August 9, 2005; 102(32): 11325 - 11330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Miller, J. M. Esparza, A. M. Lippa, F. G. Lux III, D. G. Cole, and S. K. Dutcher Mutant Kinesin-2 Motor Subunits Increase Chromosome Loss Mol. Biol. Cell, August 1, 2005; 16(8): 3810 - 3820. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. F. Lucker, R. H. Behal, H. Qin, L. C. Siron, W. D. Taggart, J. L. Rosenbaum, and D. G. Cole Characterization of the Intraflagellar Transport Complex B Core: DIRECT INTERACTION OF THE IFT81 AND IFT74/72 SUBUNITS J. Biol. Chem., July 29, 2005; 280(30): 27688 - 27696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Mueller, C. A. Perrone, R. Bower, D. G. Cole, and M. E. Porter The FLA3 KAP Subunit Is Required for Localization of Kinesin-2 to the Site of Flagellar Assembly and Processive Anterograde Intraflagellar Transport Mol. Biol. Cell, March 1, 2005; 16(3): 1341 - 1354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Redeker, N. Levilliers, E. Vinolo, J. Rossier, D. Jaillard, D. Burnette, J. Gaertig, and M.-H. Bre Mutations of Tubulin Glycylation Sites Reveal Cross-talk between the C Termini of {alpha}- and {beta}-Tubulin and Affect the Ciliary Matrix in Tetrahymena J. Biol. Chem., January 7, 2005; 280(1): 596 - 606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Mahjoub, M. Qasim Rasi, and L. M. Quarmby A NIMA-related Kinase, Fa2p, Localizes to a Novel Site in the Proximal Cilia of Chlamydomonas and Mouse Kidney Cells Mol. Biol. Cell, November 1, 2004; 15(11): 5172 - 5186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Pazour Intraflagellar Transport and Cilia-Dependent Renal Disease: The Ciliary Hypothesis of Polycystic Kidney Disease J. Am. Soc. Nephrol., October 1, 2004; 15(10): 2528 - 2536. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hou, G. J. Pazour, and G. B. Witman A Dynein Light Intermediate Chain, D1bLIC, Is Required for Retrograde Intraflagellar Transport Mol. Biol. Cell, October 1, 2004; 15(10): 4382 - 4394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-g. Li, M. Serr, E. A. Newman, and T. S. Hays The Drosophila tctex-1 Light Chain Is Dispensable for Essential Cytoplasmic Dynein Functions but Is Required during Spermatid Differentiation Mol. Biol. Cell, July 1, 2004; 15(7): 3005 - 3014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wirschell, G. Pazour, A. Yoda, M. Hirono, R. Kamiya, and G. B. Witman Oda5p, a Novel Axonemal Protein Required for Assembly of the Outer Dynein Arm and an Associated Adenylate Kinase Mol. Biol. Cell, June 1, 2004; 15(6): 2729 - 2741. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Qin, D. R. Diener, S. Geimer, D. G. Cole, and J. L. Rosenbaum Intraflagellar transport (IFT) cargo: IFT transports flagellar precursors to the tip and turnover products to the cell body J. Cell Biol., January 19, 2004; 164(2): 255 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-W. Tam, W. L. Dentler, and P. A. Lefebvre Defective flagellar assembly and length regulation in LF3 null mutants in Chlamydomonas J. Cell Biol., November 10, 2003; 163(3): 597 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Baker, K. Freeman, K. Luby-Phelps, G. J. Pazour, and J. C. Besharse IFT20 Links Kinesin II with a Mammalian Intraflagellar Transport Complex That Is Conserved in Motile Flagella and Sensory Cilia J. Biol. Chem., September 5, 2003; 278(36): 34211 - 34218. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Perrone, D. Tritschler, P. Taulman, R. Bower, B. K. Yoder, and M. E. Porter A Novel Dynein Light Intermediate Chain Colocalizes with the Retrograde Motor for Intraflagellar Transport at Sites of Axoneme Assembly in Chlamydomonas and Mammalian Cells Mol. Biol. Cell, May 1, 2003; 14(5): 2041 - 2056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Schafer, C. J. Haycraft, J. H. Thomas, B. K. Yoder, and P. Swoboda XBX-1 Encodes a Dynein Light Intermediate Chain Required for Retrograde Intraflagellar Transport and Cilia Assembly in Caenorhabditis elegans Mol. Biol. Cell, May 1, 2003; 14(5): 2057 - 2070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kathir, M. LaVoie, W. J. Brazelton, N. A. Haas, P. A. Lefebvre, and C. D. Silflow Molecular Map of the Chlamydomonas reinhardtii Nuclear Genome Eukaryot. Cell, April 1, 2003; 2(2): 362 - 379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. K. Campbell, K. G. Waymire, R. L. Heier, C. Sharer, D. E. Day, H. Reimann, J. M. Jaje, G. A. Friedrich, M. Burmeister, T. J. Bartness, et al. Mutation of a Novel Gene Results in Abnormal Development of Spermatid Flagella, Loss of Intermale Aggression and Reduced Body Fat in Mice Genetics, September 1, 2002; 162(1): 307 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Pazour, S. A. Baker, J. A. Deane, D. G. Cole, B. L. Dickert, J. L. Rosenbaum, G. B. Witman, and J. C. Besharse The intraflagellar transport protein, IFT88, is essential for vertebrate photoreceptor assembly and maintenance J. Cell Biol., April 1, 2002; 157(1): 103 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Grissom, E. A. Vaisberg, and J. R. McIntosh Identification of a Novel Light Intermediate Chain (D2LIC) for Mammalian Cytoplasmic Dynein 2 Mol. Biol. Cell, March 1, 2002; 13(3): 817 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Takada, C. G. Wilkerson, K.-i. Wakabayashi, R. Kamiya, and G. B. Witman The Outer Dynein Arm-Docking Complex: Composition and Characterization of a Subunit (Oda1) Necessary for Outer Arm Assembly Mol. Biol. Cell, March 1, 2002; 13(3): 1015 - 1029. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-F. Lechtreck, J. Rostmann, and A. Grunow Analysis of Chlamydomonas SF-assemblin by GFP tagging and expression of antisense constructs J. Cell Sci., January 4, 2002; 115(7): 1511 - 1522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Iomini, V. Babaev-Khaimov, M. Sassaroli, and G. Piperno Protein Particles in Chlamydomonas Flagella Undergo a Transport Cycle Consisting of Four Phases J. Cell Biol., March 26, 2001; 153(1): 13 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Rupp, E. O'Toole, and M. E. Porter The Chlamydomonas PF6 Locus Encodes a Large Alanine/Proline-Rich Polypeptide That Is Required for Assembly of a Central Pair Projection and Regulates Flagellar Motility Mol. Biol. Cell, March 1, 2001; 12(3): 739 - 751. [Abstract] [Full Text]
|
|
|
|
|
|
M. E. Porter and W. S. Sale The 9 + 2 Axoneme Anchors Multiple Inner Arm Dyneins and a Network of Kinases and Phosphatases that Control Motility J. Cell Biol., November 20, 2000; 151(5): F37 - F42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Boylan, M. Serr, and T. Hays A Molecular Genetic Analysis of the Interaction between the Cytoplasmic Dynein Intermediate Chain and the Glued (Dynactin) Complex Mol. Biol. Cell, November 1, 2000; 11(11): 3791 - 3803. [Abstract] [Full Text]
|
|
|
|
|
|
G. Pazour, B. Dickert, Vucica, E. Seeley, J. Rosenbaum, G. Witman, and D. Cole Chlamydomonas IFT88 and Its Mouse Homologue, Polycystic Kidney Disease Gene Tg737, Are Required for Assembly of Cilia and Flagella J. Cell Biol., October 30, 2000; 151(3): 709 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Perrone, S. H. Myster, R. Bower, E. T. O'Toole, and M. E. Porter Insights into the Structural Organization of the I1 Inner Arm Dynein from a Domain Analysis of the 1beta Dynein Heavy Chain Mol. Biol. Cell, July 1, 2000; 11(7): 2297 - 2313. [Abstract] [Full Text]
|
|
|
|
|
|
P Bastin, K Ellis, L Kohl, and K Gull Flagellum ontogeny in trypanosomes studied via an inherited and regulated RNA interference system J. Cell Sci., January 9, 2000; 113(18): 3321 - 3328. [Abstract] [PDF]
|
|
|
|
|
|
D. G. Cole Kinesin-II, Coming and Going J. Cell Biol., November 1, 1999; 147(3): 463 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Signor, K. P. Wedaman, J. T. Orozco, N. D. Dwyer, C. I. Bargmann, L. S. Rose, and J. M. Scholey Role of a Class DHC1b Dynein in Retrograde Transport of IFT Motors and IFT Raft Particles Along Cilia, but Not Dendrites, in Chemosensory Neurons of Living Caenorhabditis elegans J. Cell Biol., November 1, 1999; 147(3): 519 - 530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Brown, C. Marsala, R. Kosoy, and J. Gaertig Kinesin-II Is Preferentially Targeted to Assembling Cilia and Is Required for Ciliogenesis and Normal Cytokinesis in Tetrahymena Mol. Biol. Cell, October 1, 1999; 10(10): 3081 - 3096. [Abstract] [Full Text]
|
|
|
|
|
|
S. H. Myster, J. A. Knott, K. M. Wysocki, E. O'Toole, and M. E. Porter Domains in the 1{alpha} Dynein Heavy Chain Required for Inner Arm Assembly and Flagellar Motility in Chlamydomonas J. Cell Biol., August 23, 1999; 146(4): 801 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Bowman, R. S. Patel-King, S. E. Benashski, J. M. McCaffery, L. S.B. Goldstein, and S. M. King Drosophila roadblock and Chlamydomonas LC7: A Conserved Family of Dynein-associated Proteins Involved in Axonal Transport, Flagellar Motility, and Mitosis J. Cell Biol., July 12, 1999; 146(1): 165 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Lee, J. C. Wisniewski, W. L. Dentler, and D. J. Asai Gene Knockouts Reveal Separate Functions for Two Cytoplasmic Dyneins in Tetrahymena thermophila Mol. Biol. Cell, March 1, 1999; 10(3): 771 - 784. [Abstract] [Full Text]
|
|
|
|
|
|
P Bastin, T. Pullen, T Sherwin, and K Gull Protein transport and flagellum assembly dynamics revealed by analysis of the paralysed trypanosome mutant snl-1 J. Cell Sci., January 11, 1999; 112(21): 3769 - 3777. [Abstract] [PDF]
|
|
|
|
|
|
L. Song and W. L. Dentler Flagellar Protein Dynamics in Chlamydomonas J. Biol. Chem., August 3, 2001; 276(32): 29754 - 29763. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Pazour, S. A. Baker, J. A. Deane, D. G. Cole, B. L. Dickert, J. L. Rosenbaum, G. B. Witman, and J. C. Besharse The intraflagellar transport protein, IFT88, is essential for vertebrate photoreceptor assembly and maintenance J. Cell Biol., April 1, 2002; 157(1): 103 - 114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Carson, W. Reed, T. Lucier, L. Brighton, T. M. Gambling, C.-H. Huang, and A. M. Collier Pre- and Postnatal Lung Development, Maturation, and Plasticity: Axonemal dynein expression in human fetal tracheal epithelium Am J Physiol Lung Cell Mol Physiol, March 1, 2002; 282(3): L421 - L430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Pan and W. J. Snell Kinesin-II Is Required for Flagellar Sensory Transduction during Fertilization in Chlamydomonas Mol. Biol. Cell, April 1, 2002; 13(4): 1417 - 1426. [Abstract] [Full Text] [PDF]
|
|
