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Vol. 10, Issue 3, 693-712, March 1999
and
*Department of Cell Biology and Neuroanatomy, University of
Minnesota Medical School, Minneapolis, Minnesota 55455; and
A second cytoplasmic dynein heavy chain (cDhc) has recently been
identified in several organisms, and its expression pattern is
consistent with a possible role in axoneme assembly. We have used a genetic approach to ask whether cDhc1b is involved in flagellar assembly in Chlamydomonas. Using a modified PCR
protocol, we recovered two cDhc sequences distinct from the axonemal
Dhc sequences identified previously. cDhc1a is closely related to the
major cytoplasmic Dhc, whereas cDhc1b is closely related to the minor
cDhc isoform identified in sea urchins, Caenorhabditis
elegans, and Tetrahymena. The
Chlamydomonas cDhc1b transcript is a low-abundance mRNA
whose expression is enhanced by deflagellation. To determine its role in flagellar assembly, we screened a collection of stumpy flagellar (stf) mutants generated by insertional mutagenesis and
identified two strains in which portions of the cDhc1b
gene have been deleted. The two mutants assemble short flagellar stumps
(<1-2 µm) filled with aberrant microtubules, raft-like particles,
and other amorphous material. The results indicate that cDhc1b is
involved in the transport of components required for flagellar
assembly in Chlamydomonas.
Department of Molecular Biosciences, University of
Kansas, Lawrence, Kansas 66045
Corresponding author: 4-102 Owre
Hall, Department of Cell Biology and Neuroanatomy, 321 Church Street
Southeast, Minneapolis, MN 55455. E-mail address:
mary-p{at}biosci.cbs.umn.edu.
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|
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|
|
|
|
|
|
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|
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|
|
|
|
|
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