![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 3, 799-817, March 1999
§ and
*Department of Genetics, Stanford University School of Medicine,
Stanford, California 94305-5120; We studied the ligand-induced endocytosis of the yeast Department of
Microbiology, State University of New York, Stony Brook, New York
11794; and European Molecular Biology Laboratory, 69117 Heidelberg, Germany
-factor
receptor Ste2p by immuno-electron microscopy. We observed and
quantitated time-dependent loss of Ste2p from the plasma membrane of
cells exposed to -factor. This ligand-induced internalization of
Ste2p was blocked in the well-characterized endocytosis-deficient mutant sac6
. We provide evidence that implicates
furrow-like invaginations of the plasma membrane as the site of
receptor internalization. These invaginations are distinct from the
finger-like plasma membrane invaginations within actin cortical
patches. Consistent with this, we show that Ste2p is not located within
the cortical actin patch before and during receptor-mediated
endocytosis. In wild-type cells exposed to -factor we also observed
and quantitated a time-dependent accumulation of Ste2p in
intracellular, membrane-bound compartments. These compartments have a
characteristic electron density but variable shape and size and are
often located adjacent to the vacuole. In immuno-electron microscopy
experiments these compartments labeled with antibodies directed against
the rab5 homologue Ypt51p (Vps21p), the resident vacuolar protease
carboxypeptidase Y, and the vacuolar H+-ATPase Vph1p. Using
a new double-labeling technique we have colocalized antibodies against
Ste2p and carboxypeptidase Y to this compartment, thereby
identifying these compartments as prevacuolar late endosomes.
Corresponding author. E-mail address:
botstein{at}genome.stanford.edu.
This article has been cited by other articles:
|
|
 |
|
  F.-Z. Idrissi, H. Grotsch, I. M. Fernandez-Golbano, C. Presciatto-Baschong, H. Riezman, and M.-I. Geli Distinct acto/myosin-I structures associate with endocytic profiles at the plasma membrane J. Cell Biol., March 24, 2008; 180(6): 1219 - 1232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Moseley and B. L. Goode The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism Microbiol. Mol. Biol. Rev., September 1, 2006; 70(3): 605 - 645. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. U. Gehret, A. Bajaj, F. Naider, and M. E. Dumont Oligomerization of the Yeast {alpha}-Factor Receptor: IMPLICATIONS FOR DOMINANT NEGATIVE EFFECTS OF MUTANT RECEPTORS J. Biol. Chem., July 28, 2006; 281(30): 20698 - 20714. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Toshima, J. Toshima, M. Kaksonen, A. C. Martin, D. S. King, and D. G. Drubin Spatial dynamics of receptor-mediated endocytic trafficking in budding yeast revealed by using fluorescent {alpha}-factor derivatives PNAS, April 11, 2006; 103(15): 5793 - 5798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ren, P. Vajjhala, J. S. Lee, B. Winsor, and A. L. Munn The BAR Domain Proteins: Molding Membranes in Fission, Fusion, and Phagy Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 37 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kim, B. J. Galletta, K. O. Schmidt, F. S. Chang, K. J. Blumer, and J. A. Cooper Actin-based Motility during Endocytosis in Budding Yeast Mol. Biol. Cell, March 1, 2006; 17(3): 1354 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. S. Chang, G.-S. Han, G. M. Carman, and K. J. Blumer A WASp-binding type II phosphatidylinositol 4-kinase required for actin polymerization-driven endosome motility J. Cell Biol., October 10, 2005; 171(1): 133 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fadri, A. Daquinag, S. Wang, T. Xue, and J. Kunz The Pleckstrin Homology Domain Proteins Slm1 and Slm2 Are Required for Actin Cytoskeleton Organization in Yeast and Bind Phosphatidylinositol-4,5-Bisphosphate and TORC2 Mol. Biol. Cell, April 1, 2005; 16(4): 1883 - 1900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kirkham, A. Fujita, R. Chadda, S. J. Nixon, T. V. Kurzchalia, D. K. Sharma, R. E. Pagano, J. F. Hancock, S. Mayor, and R. G. Parton Ultrastructural identification of uncoated caveolin-independent early endocytic vehicles J. Cell Biol., January 31, 2005; 168(3): 465 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wicky, H. Schwarz, and B. Singer-Kruger Molecular Interactions of Yeast Neo1p, an Essential Member of the Drs2 Family of Aminophospholipid Translocases, and Its Role in Membrane Trafficking within the Endomembrane System Mol. Cell. Biol., September 1, 2004; 24(17): 7402 - 7418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. C. Tse, B. Mo, S. Hillmer, M. Zhao, S. W. Lo, D. G. Robinson, and L. Jiang Identification of Multivesicular Bodies as Prevacuolar Compartments in Nicotiana tabacum BY-2 Cells PLANT CELL, March 1, 2004; 16(3): 672 - 693. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. L. Yeo, L. Xu, J. Ren, V. J. Boulton, M. D. Wagle, C. Liu, G. Ren, P. Wong, R. Zahn, P. Sasajala, et al. Vps20p and Vta1p interact with Vps4p and function in multivesicular body sorting and endosomal transport in Saccharomyces cerevisiae J. Cell Sci., October 1, 2003; 116(19): 3957 - 3970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. P. Wedaman, A. Reinke, S. Anderson, J. Yates III, J. M. McCaffery, and T. Powers Tor Kinases Are in Distinct Membrane-associated Protein Complexes in Saccharomyces cerevisiae Mol. Biol. Cell, March 1, 2003; 14(3): 1204 - 1220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. T. Warren, P. D. Andrews, C. W. Gourlay, and K. R. Ayscough Sla1p couples the yeast endocytic machinery to proteins regulating actin dynamics J. Cell Sci., April 15, 2002; 115(8): 1703 - 1715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. R. Jeffries, G. W. Morgan, and M. C. Field A developmentally regulated Rab11 homologue in Trypanosoma brucei is involved in recycling processes J. Cell Sci., March 9, 2002; 114(14): 2617 - 2626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Watson, M. J. T. V. Cope, A. C. Groen, D. G. Drubin, and B. Wendland In Vivo Role for Actin-regulating Kinases in Endocytosis and Yeast Epsin Phosphorylation Mol. Biol. Cell, November 1, 2001; 12(11): 3668 - 3679. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R Zahn, B. Stevenson, S Schroder-Kohne, B Zanolari, H Riezman, and A. Munn End13p/Vps4p is required for efficient transport from early to late endosomes in Saccharomyces cerevisiae J. Cell Sci., January 5, 2001; 114(10): 1935 - 1947. [Abstract] [PDF]
|
|
|
|
|
|
E. S. Bensen, B. G. Yeung, and G. S. Payne Ric1p and the Ypt6p GTPase Function in a Common Pathway Required for Localization of Trans-Golgi Network Membrane Proteins Mol. Biol. Cell, January 1, 2001; 12(1): 13 - 26. [Abstract] [Full Text]
|
|
|
|
|
|
L. M. Ooms, B. K. McColl, F. Wiradjaja, A. P. W. Wijayaratnam, P. Gleeson, M. J. Gething, J. Sambrook, and C. A. Mitchell The Yeast Inositol Polyphosphate 5-Phosphatases Inp52p and Inp53p Translocate to Actin Patches following Hyperosmotic Stress: Mechanism for Regulating Phosphatidylinositol 4,5-Bisphosphate at Plasma Membrane Invaginations Mol. Cell. Biol., December 15, 2000; 20(24): 9376 - 9390. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Srivastava, C. A. Woolford, and E. W. Jones Pep3p/Pep5p Complex: A Putative Docking Factor at Multiple Steps of Vesicular Transport to the Vacuole of Saccharomyces cerevisiae Genetics, September 1, 2000; 156(1): 105 - 122. [Abstract] [Full Text]
|
|
|
|
|
|
A. Yesilaltay and D. D. Jenness Homo-oligomeric Complexes of the Yeast alpha -Factor Pheromone Receptor Are Functional Units of Endocytosis Mol. Biol. Cell, September 1, 2000; 11(9): 2873 - 2884. [Abstract] [Full Text]
|
|
|
|
|
|
M. J. Conboy and M. S. Cyert Luv1p/Rki1p/Tcs3p/Vps54p, a Yeast Protein That Localizes to the Late Golgi and Early Endosome, Is Required for Normal Vacuolar Morphology. Mol. Biol. Cell, July 1, 2000; 11(7): 2429 - 2443. [Abstract] [Full Text]
|
|
|
|
|
|
W.-j. Luo and A. Chang An Endosome-to-Plasma Membrane Pathway Involved in Trafficking of a Mutant Plasma Membrane ATPase in Yeast Mol. Biol. Cell, February 1, 2000; 11(2): 579 - 592. [Abstract] [Full Text]
|
|
|
|
|
|
S. R. Gerrard, N. J. Bryant, and T. H. Stevens VPS21 Controls Entry of Endocytosed and Biosynthetic Proteins into the Yeast Prevacuolar Compartment Mol. Biol. Cell, February 1, 2000; 11(2): 613 - 626. [Abstract] [Full Text]
|
|
|
|
|
|
B Gagny, A Wiederkehr, P Dumoulin, B Winsor, H Riezman, and R Haguenauer-Tsapis A novel EH domain protein of Saccharomyces cerevisiae, Ede1p, involved in endocytosis J. Cell Sci., January 9, 2000; 113(18): 3309 - 3319. [Abstract] [PDF]
|
|
|
|
|
|
D Pruyne and A Bretscher Polarization of cell growth in yeast J. Cell Sci., January 2, 2000; 113(4): 571 - 585. [Abstract] [PDF]
|
|
|
|
|
|
C.-Y. Chen, M. F. Ingram, P. H. Rosal, and T. R. Graham Role for Drs2p, a P-Type ATPase and Potential Aminophospholipid Translocase, in Yeast Late Golgi Function J. Cell Biol., December 13, 1999; 147(6): 1223 - 1236. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Schott, J. Ho, D. Pruyne, and A. Bretscher The COOH-Terminal Domain of Myo2p, a Yeast Myosin V, Has a Direct Role in Secretory Vesicle Targeting J. Cell Biol., November 15, 1999; 147(4): 791 - 808. [Abstract] [Full Text] [PDF]
|
|
