![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 4, 1119-1131, April 1999
-Catenin Can Be Transported into the Nucleus in a
Ran-unassisted Manner
Department of Anatomy and Cell Biology, Osaka University Medical
School, Osaka 565-0871, Japan
The nuclear accumulation of 
-catenin plays an important role in
the Wingless/Wnt signaling pathway. This study describes an examination
of the nuclear import of -catenin in living mammalian cells and in
vitro semi-intact cells. When injected into the cell cytoplasm,
-catenin rapidly migrated into the nucleus in a
temperature-dependent and wheat germ agglutinin-sensitive manner. In
the cell-free import assay, -catenin rapidly migrates into the
nucleus without the exogenous addition of cytosol, Ran, or ATP/GTP.
Cytoplasmic injection of mutant Ran defective in its GTP hydrolysis did
not prevent -catenin import. Studies using tsBN2, a
temperature-sensitive mutant cell line that possesses a point mutation
in the RCC1 gene, showed that the import of -catenin is insensitive
to nuclear Ran-GTP depletion. These results show that -catenin
possesses the ability to constitutively translocate through the nuclear pores in a manner similar to importin in a Ran-unassisted manner. We further showed that -catenin also rapidly exits the nucleus in
homokaryons, suggesting that the regulation of nuclear levels of
-catenin involves both nuclear import and export of this molecule.
Corresponding author. E-mail address:
yyoneda{at}anat3.med.osaka-u.ac.jp.
This article has been cited by other articles:
|
|
 |
|
  Y. Hayashi-Takanaka, K. Yamagata, N. Nozaki, and H. Kimura Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase J. Cell Biol., December 14, 2009; 187(6): 781 - 790. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Vazquez-Iglesias, I. Lostale-Seijo, J. Martinez-Costas, and J. Benavente Avian Reovirus SigmaA Localizes to the Nucleolus and Enters the Nucleus by a Nonclassical Energy- and Carrier-Independent Pathway J. Virol., October 1, 2009; 83(19): 10163 - 10175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kitagawa, K.-I. Matsuda, S. Inui, H. Takenaka, N. Katoh, S. Itami, S. Kishimoto, and M. Kawata Keratinocyte Growth Inhibition through the Modification of Wnt Signaling by Androgen in Balding Dermal Papilla Cells J. Clin. Endocrinol. Metab., April 1, 2009; 94(4): 1288 - 1294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.-Q. Li, A. Mofunanya, K. Harris, and K.-I. Takemaru Chibby cooperates with 14-3-3 to regulate {beta}-catenin subcellular distribution and signaling activity J. Cell Biol., October 22, 2008; 181(7): 1141 - 1154. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Dorfman and I. G. Macara STRAD{alpha} Regulates LKB1 Localization by Blocking Access to Importin-{alpha}, and by Association with Crm1 and Exportin-7 Mol. Biol. Cell, April 1, 2008; 19(4): 1614 - 1626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Tsuji, N. Sheehy, V. W. Gautier, H. Hayakawa, H. Sawa, and W. W. Hall The Nuclear Import of the Human T Lymphotropic Virus Type I (HTLV-1) Tax Protein Is Carrier- and Energy-independent J. Biol. Chem., May 4, 2007; 282(18): 13875 - 13883. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nakahara, N. Oka, Y. Wang, V. Hogan, H. Inohara, and A. Raz Characterization of the nuclear import pathways of galectin-3. Cancer Res., October 15, 2006; 66(20): 9995 - 10006. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Krieghoff, J. Behrens, and B. Mayr Nucleo-cytoplasmic distribution of {beta}-catenin is regulated by retention. J. Cell Sci., April 1, 2006; 119(Pt 7): 1453 - 1463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Miyauchi, T. Michigami, N. Sakaguchi, T. Sekimoto, Y. Yoneda, J. W. Pike, M. Yamagata, and K. Ozono Importin 4 Is Responsible for Ligand-independent Nuclear Translocation of Vitamin D Receptor J. Biol. Chem., December 9, 2005; 280(49): 40901 - 40908. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Massague, J. Seoane, and D. Wotton Smad transcription factors Genes & Dev., December 1, 2005; 19(23): 2783 - 2810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kose, M. Furuta, M. Koike, Y. Yoneda, and N. Imamoto The 70-kD heat shock cognate protein (hsc70) facilitates the nuclear export of the import receptors J. Cell Biol., October 10, 2005; 171(1): 19 - 25. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Qi, N. Chen, J. Wang, and C.-H. Siu Transendothelial Migration of Melanoma Cells Involves N-Cadherin-mediated Adhesion and Activation of the {beta}-Catenin Signaling Pathway Mol. Biol. Cell, September 1, 2005; 16(9): 4386 - 4397. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sakakida, Y. Miyamoto, E. Nagoshi, M. Akashi, T. J. Nakamura, T. Mamine, M. Kasahara, Y. Minami, Y. Yoneda, and T. Takumi Importin {alpha}/{beta} Mediates Nuclear Transport of a Mammalian Circadian Clock Component, mCRY2, Together with mPER2, through a Bipartite Nuclear Localization Signal J. Biol. Chem., April 8, 2005; 280(14): 13272 - 13278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Gao, S. Jin, Y. Song, M. Fu, M. Wang, Z. Liu, M. Wu, and Q. Zhan B23 Regulates GADD45a Nuclear Translocation and Contributes to GADD45a-induced Cell Cycle G2-M Arrest J. Biol. Chem., March 25, 2005; 280(12): 10988 - 10996. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhong, A. Takeda, R. Nazari, H. Shio, G. Blobel, and N. R. Yaseen Carrier-independent Nuclear Import of the Transcription Factor PU.1 via RanGTP-stimulated Binding to Nup153 J. Biol. Chem., March 18, 2005; 280(11): 10675 - 10682. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Moriguchi, T. Suzuki, Y. Ito, Y. Yamazaki, Y. Niwa, and N. Kurata Functional Isolation of Novel Nuclear Proteins Showing a Variety of Subnuclear Localizations PLANT CELL, February 1, 2005; 17(2): 389 - 403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Koike, S. Kose, M. Furuta, N. Taniguchi, F. Yokoya, Y. Yoneda, and N. Imamoto {beta}-Catenin Shows an Overlapping Sequence Requirement but Distinct Molecular Interactions for Its Bidirectional Passage through Nuclear Pores J. Biol. Chem., August 6, 2004; 279(32): 34038 - 34047. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Chen, A. M. Brownawell, and I. G. Macara Nucleocytoplasmic Shuttling of JAZ, a New Cargo Protein for Exportin-5 Mol. Cell. Biol., August 1, 2004; 24(15): 6608 - 6619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Furuta, S. Kose, M. Koike, T. Shimi, Y. Hiraoka, Y. Yoneda, T. Haraguchi, and N. Imamoto Heat-shock induced nuclear retention and recycling inhibition of importin {alpha} Genes Cells, May 1, 2004; 9(5): 429 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Cong and H. Varmus Nuclear-cytoplasmic shuttling of Axin regulates subcellular localization of {beta}-catenin PNAS, March 2, 2004; 101(9): 2882 - 2887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, M. Yamada, N. Mabuchi, and H. Shida Cellular Requirements for CRM1 Import and Export J. Biochem., November 1, 2003; 134(5): 759 - 764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Roczniak-Ferguson and A. B. Reynolds Regulation of p120-catenin nucleocytoplasmic shuttling activity J. Cell Sci., October 15, 2003; 116(20): 4201 - 4212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tanaka, M. Nishi, M. Morimoto, T. Sugimoto, and M. Kawata Yellow Fluorescent Protein-Tagged and Cyan Fluorescent Protein-Tagged Imaging Analysis of Glucocorticoid Receptor and Importins in Single Living Cells Endocrinology, September 1, 2003; 144(9): 4070 - 4079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Wang, Y. Wu, X. Ge, L. Ma, and G. Pei Subcellular Localization of beta -Arrestins Is Determined by Their Intact N Domain and the Nuclear Export Signal at the C Terminus J. Biol. Chem., March 21, 2003; 278(13): 11648 - 11653. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Li and R. Iyengar Calpain as an effector of the Gq signaling pathway for inhibition of Wnt/beta -catenin-regulated cell proliferation PNAS, October 1, 2002; 99(20): 13254 - 13259. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Lee, K. A. D'Amour, and J. Papkoff A yeast model system for functional analysis of {beta}-catenin signaling J. Cell Biol., September 16, 2002; 158(6): 1067 - 1078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. D. Schwoebel, T. H. Ho, and M. S. Moore The mechanism of inhibition of Ran-dependent nuclear transport by cellular ATP depletion J. Cell Biol., June 10, 2002; 157(6): 963 - 974. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Pawlowski, J. R. Ertel, M. P. Allen, M. Xu, C. Butler, E. M. Wilson, and M. E. Wierman Liganded Androgen Receptor Interaction with beta -Catenin. NUCLEAR CO-LOCALIZATION AND MODULATION OF TRANSCRIPTIONAL ACTIVITY IN NEURONAL CELLS J. Biol. Chem., May 31, 2002; 277(23): 20702 - 20710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Whitehurst, J. L. Wilsbacher, Y. You, K. Luby-Phelps, M. S. Moore, and M. H. Cobb ERK2 enters the nucleus by a carrier-independent mechanism PNAS, May 28, 2002; 99(11): 7496 - 7501. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Weng, M. Xin, L. Pablo, D. Grueneberg, M. Hagel, G. Bain, T. Muller, and J. Papkoff Protection against Anoikis and Down-regulation of Cadherin Expression by a Regulatable beta -Catenin Protein J. Biol. Chem., May 17, 2002; 277(21): 18677 - 18686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Mulholland, H. Cheng, K. Reid, P. S. Rennie, and C. C. Nelson The Androgen Receptor Can Promote beta -Catenin Nuclear Translocation Independently of Adenomatous Polyposis Coli J. Biol. Chem., May 10, 2002; 277(20): 17933 - 17943. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Poupon, S. Polo, M. Vecchi, G. Martin, A. Dautry-Varsat, N. Cerf-Bensussan, P. P. Di Fiore, and A. Benmerah Differential Nucleocytoplasmic Trafficking between the Related Endocytic Proteins Eps15 and Eps15R J. Biol. Chem., March 8, 2002; 277(11): 8941 - 8948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. G. Macara Transport into and out of the Nucleus Microbiol. Mol. Biol. Rev., December 1, 2001; 65(4): 570 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Matsubayashi, M. Fukuda, and E. Nishida Evidence for Existence of a Nuclear Pore Complex-mediated, Cytosol-independent Pathway of Nuclear Translocation of ERK MAP Kinase in Permeabilized Cells J. Biol. Chem., November 2, 2001; 276(45): 41755 - 41760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Meng, R. Poon, X. Zhang, A. Cheah, Q. Ding, C.-c. Hui, and B. Alman Suppressor of Fused Negatively Regulates beta -Catenin Signaling J. Biol. Chem., October 19, 2001; 276(43): 40113 - 40119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. S. Tolwinski and E. Wieschaus Armadillo nuclear import is regulated by cytoplasmic anchor Axin and nuclear anchor dTCF/Pan Development, June 1, 2001; 128(11): 2107 - 2117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Brownawell, G. J. P. L. Kops, I. G. Macara, and B. M. T. Burgering Inhibition of Nuclear Import by Protein Kinase B (Akt) Regulates the Subcellular Distribution and Activity of the Forkhead Transcription Factor AFX Mol. Cell. Biol., May 15, 2001; 21(10): 3534 - 3546. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Lo Muzio A Possible Role for the Wnt~1 Pathway in Oral Carcinogenesis Critical Reviews in Oral Biology & Medicine, January 1, 2001; 12(2): 152 - 165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sakaguchi, M. Miyazaki, Y. Inoue, T. Tsuji, H. Kouchi, T. Tanaka, H. Yamada, and M. Namba Relationship between Contact Inhibition and Intranuclear S100c of Normal Human Fibroblasts J. Cell Biol., June 12, 2000; 149(6): 1193 - 1206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Nemergut and I. G. Macara Nuclear Import of the Ran Exchange Factor, Rcc1, Is Mediated by at Least Two Distinct Mechanisms J. Cell Biol., May 15, 2000; 149(4): 835 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sachdev, S. Bagchi, D. D. Zhang, A. C. Mings, and M. Hannink Nuclear Import of Ikappa Balpha Is Accomplished by a Ran-Independent Transport Pathway Mol. Cell. Biol., March 1, 2000; 20(5): 1571 - 1582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hetzer and I. W. Mattaj An Atp-Dependent, Ran-Independent Mechanism for Nuclear Import of the U1a and U2b'' Spliceosome Proteins J. Cell Biol., January 24, 2000; 148(2): 293 - 304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Zhurinsky, M Shtutman, and A Ben-Ze'ev Plakoglobin and beta-catenin: protein interactions, regulation and biological roles J. Cell Sci., January 9, 2000; 113(18): 3127 - 3139. [Abstract] [PDF]
|
|
|
|
|
|
T Haraguchi, T Koujin, T Hayakawa, T Kaneda, C Tsutsumi, N Imamoto, C Akazawa, J Sukegawa, Y Yoneda, and Y Hiraoka Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes J. Cell Sci., January 3, 2000; 113(5): 779 - 794. [Abstract] [PDF]
|
|
|
|
|
|
A. L. Giannini, M. d. M. Vivanco, and R. M. Kypta alpha -Catenin Inhibits beta -Catenin Signaling by Preventing Formation of a beta -Catenin{middle dot}T-cell Factor{middle dot}DNA Complex J. Biol. Chem., July 14, 2000; 275(29): 21883 - 21888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Schedlich, S. L. Le Page, S. M. Firth, L. J. Briggs, D. A. Jans, and R. C. Baxter Nuclear Import of Insulin-like Growth Factor-binding Protein-3 and -5 Is Mediated by the Importin beta Subunit J. Biol. Chem., July 28, 2000; 275(31): 23462 - 23470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-J. Jiang, K. Shoji, R. Matsuki, A. Baba, N. Inagaki, H. Ban, T. Iwasaki, N. Imamoto, Y. Yoneda, X.-W. Deng, et al. Molecular Cloning of a Novel Importin alpha Homologue from Rice, by Which Constitutive Photomorphogenic 1 (COP1) Nuclear Localization Signal (NLS)-Protein Is Preferentially Nuclear Imported J. Biol. Chem., March 16, 2001; 276(12): 9322 - 9329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Toyotome, T. Suzuki, A. Kuwae, T. Nonaka, H. Fukuda, S. Imajoh-Ohmi, T. Toyofuku, M. Hori, and C. Sasakawa Shigella Protein IpaH9.8 Is Secreted from Bacteria within Mammalian Cells and Transported to the Nucleus J. Biol. Chem., August 17, 2001; 276(34): 32071 - 32079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Eleftheriou, M. Yoshida, and B. R. Henderson Nuclear Export of Human beta -Catenin Can Occur Independent of CRM1 and the Adenomatous Polyposis Coli Tumor Suppressor J. Biol. Chem., July 6, 2001; 276(28): 25883 - 25888. [Abstract] [Full Text] [PDF]
|
|
