![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 4, 961-974, April 1999
-Cyclodextrin Perturbs
Formation of Clathrin-coated Endocytic Vesicles
and
*Institute for Cancer Research, The Norwegian Radium Hospital,
Montebello, 0310 Oslo, Norway; and The importance of cholesterol for endocytosis has been investigated
in HEp-2 and other cell lines by using methyl-Structural Cell
Biology Unit, Department of Medical Anatomy, The Panum Institute,
University of Copenhagen, DK-2200 Copenhagen N, Denmark
-cyclodextrin (MCD)
to selectively extract cholesterol from the plasma membrane. MCD
treatment strongly inhibited endocytosis of transferrin and EGF,
whereas endocytosis of ricin was less affected. The inhibition of
transferrin endocytosis was completely reversible. On removal of MCD
it was restored by continued incubation of the cells even in serum-free
medium. The recovery in serum-free medium was inhibited by addition of
lovastatin, which prevents cholesterol synthesis, but endocytosis
recovered when a water-soluble form of cholesterol was added together
with lovastatin. Electron microscopical studies of MCD-treated HEp-2
cells revealed that typical invaginated caveolae were no longer
present. Moreover, the invagination of clathrin-coated pits was
strongly inhibited, resulting in accumulation of shallow coated pits.
Quantitative immunogold labeling showed that transferrin receptors were
concentrated in coated pits to the same degree (approximately
sevenfold) after MCD treatment as in control cells. Our results
therefore indicate that although clathrin-independent (and
caveolae-independent) endocytosis still operates after removal of
cholesterol, cholesterol is essential for the formation of
clathrin-coated endocytic vesicles.
Corresponding author. E-mail
address: ksandvig{at}radium.uio.no.
This article has been cited by other articles:
|
|
 |
|
  N. H. Petersen, N. J. Faegeman, L. Yu, and D. Wustner Kinetic imaging of NPC1L1 and sterol trafficking between plasma membrane and recycling endosomes in hepatoma cells J. Lipid Res., September 1, 2008; 49(9): 2023 - 2037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Lei and M. Martinez-Moczygemba Separate endocytic pathways regulate IL-5 receptor internalization and signaling J. Leukoc. Biol., August 1, 2008; 84(2): 499 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Zheng, J. Chu, Y. Qiu, H. H. Loh, and P.-Y. Law Agonist-selective signaling is determined by the receptor location within the membrane domains PNAS, July 8, 2008; 105(27): 9421 - 9426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Medigeshi, A. J. Hirsch, D. N. Streblow, J. Nikolich-Zugich, and J. A. Nelson West Nile Virus Entry Requires Cholesterol-Rich Membrane Microdomains and Is Independent of {alpha}v{beta}3 Integrin J. Virol., June 1, 2008; 82(11): 5212 - 5219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Schumann, J F Richter, I Wedell, V Moos, M Zimmermann-Kordmann, T Schneider, S Daum, M Zeitz, M Fromm, and J D Schulzke Mechanisms of epithelial translocation of the {alpha}2-gliadin-33mer in coeliac sprue Gut, June 1, 2008; 57(6): 747 - 754. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Konopka, S. K. Backues, and S. Y. Bednarek Dynamics of Arabidopsis Dynamin-Related Protein 1C and a Clathrin Light Chain at the Plasma Membrane PLANT CELL, May 1, 2008; 20(5): 1363 - 1380. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. K. Juckem, K. W. Boehme, A. L. Feire, and T. Compton Differential Initiation of Innate Immune Responses Induced by Human Cytomegalovirus Entry into Fibroblast Cells J. Immunol., April 1, 2008; 180(7): 4965 - 4977. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhang, F. Tan, Y. Zhang, and R. A. Skidgel Carboxypeptidase M and Kinin B1 Receptors Interact to Facilitate Efficient B1 Signaling from B2 Agonists J. Biol. Chem., March 21, 2008; 283(12): 7994 - 8004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Setiadi and R. P. McEver Clustering endothelial E-selectin in clathrin-coated pits and lipid rafts enhances leukocyte adhesion under flow Blood, February 15, 2008; 111(4): 1989 - 1998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Rojek, M. Perez, and S. Kunz Cellular Entry of Lymphocytic Choriomeningitis Virus J. Virol., February 1, 2008; 82(3): 1505 - 1517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Chen, L. Steinhauer, J. Hammerlindl, W. Keller, and J. Zou Biosynthesis of Phytosterol Esters: Identification of a Sterol O-Acyltransferase in Arabidopsis Plant Physiology, November 1, 2007; 145(3): 974 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Eifart, K. Ludwig, C. Bottcher, C. A. M. de Haan, P. J. M. Rottier, T. Korte, and A. Herrmann Role of Endocytosis and Low pH in Murine Hepatitis Virus Strain A59 Cell Entry J. Virol., October 1, 2007; 81(19): 10758 - 10768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. A. Zemskov, I. Mikhailenko, D. K. Strickland, and A. M. Belkin Cell-surface transglutaminase undergoes internalization and lysosomal degradation: an essential role for LRP1 J. Cell Sci., September 15, 2007; 120(18): 3188 - 3199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.-Y. Cho, D.-I. Cho, J. H. Park, H. Kurose, M. G. Caron, and K.-M. Kim Roles of Protein Kinase C and Actin-Binding Protein 280 in the Regulation of Intracellular Trafficking of Dopamine D3 Receptor Mol. Endocrinol., September 1, 2007; 21(9): 2242 - 2254. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Inoue, N. Tanaka, Y. Tanaka, S. Inoue, K. Morita, M. Zhuang, T. Hattori, and K. Sugamura Clathrin-Dependent Entry of Severe Acute Respiratory Syndrome Coronavirus into Target Cells Expressing ACE2 with the Cytoplasmic Tail Deleted J. Virol., August 15, 2007; 81(16): 8722 - 8729. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tateno, H. Li, M. J. Schur, N. Bovin, P. R. Crocker, W. W. Wakarchuk, and J. C. Paulson Distinct Endocytic Mechanisms of CD22 (Siglec-2) and Siglec-F Reflect Roles in Cell Signaling and Innate Immunity Mol. Cell. Biol., August 15, 2007; 27(16): 5699 - 5710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Martinez, S. M. Cordo, and N. A. Candurra Characterization of Junin arenavirus cell entry J. Gen. Virol., June 1, 2007; 88(6): 1776 - 1784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Torgersen, S. Walchli, S. Grimmer, S. S. Skanland, and K. Sandvig Protein Kinase C{delta} Is Activated by Shiga Toxin and Regulates Its Transport J. Biol. Chem., June 1, 2007; 282(22): 16317 - 16328. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Leyt, N. Melamed-Book, J.-P. Vaerman, S. Cohen, A. M. Weiss, and B. Aroeti Cholesterol-sensitive Modulation of Transcytosis Mol. Biol. Cell, June 1, 2007; 18(6): 2057 - 2071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wang, L. Yang, and H. W. Huang Evidence of Cholesterol Accumulated in High Curvature Regions: Implication to the Curvature Elastic Energy for Lipid Mixtures Biophys. J., April 15, 2007; 92(8): 2819 - 2830. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Wasser, M. Ertunc, X. Liu, and E. T. Kavalali Cholesterol-dependent balance between evoked and spontaneous synaptic vesicle recycling J. Physiol., March 1, 2007; 579(2): 413 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cantin, J. Holguera, L. Ferreira, E. Villar, and I. Munoz-Barroso Newcastle disease virus may enter cells by caveolae-mediated endocytosis J. Gen. Virol., February 1, 2007; 88(2): 559 - 569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hinzpeter, J. Fritsch, F. Borot, S. Trudel, D.-L. Vieu, F. Brouillard, M. Baudouin-Legros, J. Clain, A. Edelman, and M. Ollero Membrane Cholesterol Content Modulates ClC-2 Gating and Sensitivity to Oxidative Stress J. Biol. Chem., January 26, 2007; 282(4): 2423 - 2432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Holzer and S. B. Howell The Internalization and Degradation of Human Copper Transporter 1 following Cisplatin Exposure. Cancer Res., November 15, 2006; 66(22): 10944 - 10952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-y. Jia, S. Lamer, M. Schumann, M. R. Schmidt, E. Krause, and V. Haucke Quantitative Proteomics Analysis of Detergent-resistant Membranes from Chemical Synapses: Evidence for Cholesterol as Spatial Organizer of Synaptic Vesicle Cycling Mol. Cell. Proteomics, November 1, 2006; 5(11): 2060 - 2071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Weangsripanaval, K. Murota, Y. Murakami, M. Kominami, T. Kusudo, T. Moriyama, T. Ogawa, and T. Kawada Sodium Cromoglycate Inhibits Absorption of the Major Soybean Allergen, Gly m Bd 30K, in Mice and Human Intestinal Caco-2 Cells J. Nutr., November 1, 2006; 136(11): 2874 - 2880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Idkowiak-Baldys, K. P. Becker, K. Kitatani, and Y. A. Hannun Dynamic Sequestration of the Recycling Compartment by Classical Protein Kinase C J. Biol. Chem., August 4, 2006; 281(31): 22321 - 22331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Stuart and T. D. K. Brown Entry of feline calicivirus is dependent on clathrin-mediated endocytosis and acidification in endosomes. J. Virol., August 1, 2006; 80(15): 7500 - 7509. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Garcion, A. Lamprecht, B. Heurtault, A. Paillard, A. Aubert-Pouessel, B. Denizot, P. Menei, and J.-P. Benoit A new generation of anticancer, drug-loaded, colloidal vectors reverses multidrug resistance in glioma and reduces tumor progression in rats. Mol. Cancer Ther., July 1, 2006; 5(7): 1710 - 1722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Lambot, P. Lybaert, A. Boom, J. Delogne-Desnoeck, A.M. Vanbellinghen, G. Graff, P. Lebrun, and S. Meuris Evidence for a Clathrin-Mediated Recycling of Albumin in Human Term Placenta Biol Reprod, July 1, 2006; 75(1): 90 - 97. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Russo, M. McKee, and D. Brown Methyl-beta-cyclodextrin induces vasopressin-independent apical accumulation of aquaporin-2 in the isolated, perfused rat kidney Am J Physiol Renal Physiol, July 1, 2006; 291(1): F246 - F253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Hinrichsen, A. Meyerholz, S. Groos, and E. J. Ungewickell Bending a membrane: How clathrin affects budding PNAS, June 6, 2006; 103(23): 8715 - 8720. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. U. Lauvrak, S. Walchli, T.-G. Iversen, H. H. Slagsvold, M. L. Torgersen, B. Spilsberg, and K. Sandvig Shiga Toxin Regulates Its Entry in a Syk-dependent Manner Mol. Biol. Cell, March 1, 2006; 17(3): 1096 - 1109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nazli, A. Wang, O. Steen, D. Prescott, J. Lu, M. H. Perdue, J. D. Soderholm, P. M. Sherman, and D. M. McKay Enterocyte Cytoskeleton Changes Are Crucial for Enhanced Translocation of Nonpathogenic Escherichia coli across Metabolically Stressed Gut Epithelia Infect. Immun., January 1, 2006; 74(1): 192 - 201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kasai, T. Shima, and M. Okada Role of Src family tyrosine kinases in the down-regulation of epidermal growth factor signaling in PC12 cells Genes Cells, December 1, 2005; 10(12): 1175 - 1187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Zang, S. Kalache, M. Lin, B. Schroppel, and B. Murphy MHC Class II-Mediated Apoptosis by a Nonpolymorphic MHC Class II Peptide Proceeds by Activation of Protein Kinase C J. Am. Soc. Nephrol., December 1, 2005; 16(12): 3661 - 3668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kalvodova, N. Kahya, P. Schwille, R. Ehehalt, P. Verkade, D. Drechsel, and K. Simons Lipids as Modulators of Proteolytic Activity of BACE: INVOLVEMENT OF CHOLESTEROL, GLYCOSPHINGOLIPIDS, AND ANIONIC PHOSPHOLIPIDS IN VITRO J. Biol. Chem., November 4, 2005; 280(44): 36815 - 36823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. I. Plotkin, I. Mathov, J. I. Aguirre, A. M. Parfitt, S. C. Manolagas, and T. Bellido Mechanical stimulation prevents osteocyte apoptosis: requirement of integrins, Src kinases, and ERKs Am J Physiol Cell Physiol, September 1, 2005; 289(3): C633 - C643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Orr, D. Hu, S. Ozcelik, L. K. Opresko, H. S. Wiley, and S. D. Colson Cholesterol Dictates the Freedom of EGF Receptors and HER2 in the Plane of the Membrane Biophys. J., August 1, 2005; 89(2): 1362 - 1373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Y. Breusegem, N. Halaihel, M. Inoue, H. Zajicek, E. Lederer, N. P. Barry, V. Sorribas, and M. Levi Acute and chronic changes in cholesterol modulate Na-Pi cotransport activity in OK cells Am J Physiol Renal Physiol, July 1, 2005; 289(1): F154 - F165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-I. Yim, S. Scarselletta, F. Zang, X. Wu, D.-w. Lee, Y.-s. Kang, E. Eisenberg, and L. E. Greene Exchange of clathrin, AP2 and epsin on clathrin-coated pits in permeabilized tissue culture cells J. Cell Sci., June 1, 2005; 118(11): 2405 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Cole, A. Grudzien, I. O. Manhart, B. L. Kelly, H. Oakley, and R. Vassar Statins Cause Intracellular Accumulation of Amyloid Precursor Protein, {beta}-Secretase-cleaved Fragments, and Amyloid {beta}-Peptide via an Isoprenoid-dependent Mechanism J. Biol. Chem., May 13, 2005; 280(19): 18755 - 18770. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Herincs, V. Corset, N. Cahuzac, C. Furne, V. Castellani, A.-O. Hueber, and P. Mehlen DCC association with lipid rafts is required for netrin-1-mediated axon guidance J. Cell Sci., April 15, 2005; 118(8): 1687 - 1692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. P. Richard, K. Melikov, H. Brooks, P. Prevot, B. Lebleu, and L. V. Chernomordik Cellular Uptake of Unconjugated TAT Peptide Involves Clathrin-dependent Endocytosis and Heparan Sulfate Receptors J. Biol. Chem., April 15, 2005; 280(15): 15300 - 15306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Krey, H.-J. Thiel, and T. Rumenapf Acid-Resistant Bovine Pestivirus Requires Activation for pH-Triggered Fusion during Entry J. Virol., April 1, 2005; 79(7): 4191 - 4200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Signoret, L. Hewlett, S. Wavre, A. Pelchen-Matthews, M. Oppermann, and M. Marsh Agonist-induced Endocytosis of CC Chemokine Receptor 5 Is Clathrin Dependent Mol. Biol. Cell, February 1, 2005; 16(2): 902 - 917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Harikumar, V. Puri, R. D. Singh, K. Hanada, R. E. Pagano, and L. J. Miller Differential Effects of Modification of Membrane Cholesterol and Sphingolipids on the Conformation, Function, and Trafficking of the G Protein-coupled Cholecystokinin Receptor J. Biol. Chem., January 21, 2005; 280(3): 2176 - 2185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Walrafen, F. Verdier, Z. Kadri, S. Chretien, C. Lacombe, and P. Mayeux Both proteasomes and lysosomes degrade the activated erythropoietin receptor Blood, January 15, 2005; 105(2): 600 - 608. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Gabel, C. Elwell, S. C. D. van Ijzendoorn, and J. N. Engel Lipid Raft-Mediated Entry Is Not Required for Chlamydia trachomatis Infection of Cultured Epithelial Cells Infect. Immun., December 1, 2004; 72(12): 7367 - 7373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Balbis, G. Baquiran, C. Mounier, and B. I. Posner Effect of Insulin on Caveolin-enriched Membrane Domains in Rat Liver J. Biol. Chem., September 17, 2004; 279(38): 39348 - 39357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Mitchell, A. Choudhury, R. E. Pagano, and E. B. Leof Ligand-dependent and -independent Transforming Growth Factor-{beta} Receptor Recycling Regulated by Clathrin-mediated Endocytosis and Rab11 Mol. Biol. Cell, September 1, 2004; 15(9): 4166 - 4178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Naslavsky, R. Weigert, and J. G. Donaldson Characterization of a Nonclathrin Endocytic Pathway: Membrane Cargo and Lipid Requirements Mol. Biol. Cell, August 1, 2004; 15(8): 3542 - 3552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Massol, J. E. Larsen, Y. Fujinaga, W. I. Lencer, and T. Kirchhausen Cholera Toxin Toxicity Does Not Require Functional Arf6- and Dynamin-dependent Endocytic Pathways Mol. Biol. Cell, August 1, 2004; 15(8): 3631 - 3641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Tkachenko, E. Lutgens, R.-V. Stan, and M. Simons Fibroblast growth factor 2 endocytosis in endothelial cells proceed via syndecan-4-dependent activation of Rac1 and a Cdc42-dependent macropinocytic pathway J. Cell Sci., July 1, 2004; 117(15): 3189 - 3199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Beh and J. Rine A role for yeast oxysterol-binding protein homologs in endocytosis and in the maintenance of intracellular sterol-lipid distribution J. Cell Sci., June 15, 2004; 117(14): 2983 - 2996. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Kenworthy, B. J. Nichols, C. L. Remmert, G. M. Hendrix, M. Kumar, J. Zimmerberg, and J. Lippincott-Schwartz Dynamics of putative raft-associated proteins at the cell surface J. Cell Biol., June 7, 2004; 165(5): 735 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Wang, J. Dufner-Beattie, B.-E. Kim, M. J. Petris, G. Andrews, and D. J. Eide Zinc-stimulated Endocytosis Controls Activity of the Mouse ZIP1 and ZIP3 Zinc Uptake Transporters J. Biol. Chem., June 4, 2004; 279(23): 24631 - 24639. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Wenk and P. De Camilli Inaugural Article: Protein-lipid interactions and phosphoinositide metabolism in membrane traffic: Insights from vesicle recycling in nerve terminals PNAS, June 1, 2004; 101(22): 8262 - 8269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Sadler and N. D. Jacobs Stimulation of Xenopus laevis Oocyte Maturation by Methyl-{beta}-Cyclodextrin Biol Reprod, June 1, 2004; 70(6): 1685 - 1692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Siddiqui, Z. K. Siddiqui, and A. B. Malik Albumin endocytosis in endothelial cells induces TGF-{beta} receptor II signaling Am J Physiol Lung Cell Mol Physiol, May 1, 2004; 286(5): L1016 - L1026. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 |
