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Vol. 10, Issue 4, 987-1000, April 1999
Howard Hughes Medical Institute and Department of Biochemistry and
Biophysics, University of California, School of Medicine, San
Francisco, California 94143-0448
The TOR (target of rapamycin) signal transduction pathway is an
important mechanism by which cell growth is controlled in all
eucaryotic cells. Specifically, TOR signaling adjusts the protein
biosynthetic capacity of cells according to nutrient availability. In
mammalian cells, one branch of this pathway controls general translational initiation, whereas a separate branch specifically regulates the translation of ribosomal protein (r-protein) mRNAs. In
Saccharomyces cerevisiae, the TOR pathway similarly
regulates general translational initiation, but its specific role in
the synthesis of ribosomal components is not well understood. Here we
demonstrate that in yeast control of ribosome biosynthesis by the TOR
pathway is surprisingly complex. In addition to general effects on
translational initiation, TOR exerts drastic control over r-protein
gene transcription as well as the synthesis and subsequent processing
of 35S precursor rRNA. We also find that TOR signaling is a
prerequisite for the induction of r-protein gene transcription that
occurs in response to improved nutrient conditions. This induction has
been shown previously to involve both the Ras-adenylate cyclase
as well as the fermentable growth medium-induced pathways, and
our results therefore suggest that these three pathways may be
intimately linked.
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Y. Uesono and A. Toh-e Transient Inhibition of Translation Initiation by Osmotic Stress J. Biol. Chem., April 12, 2002; 277(16): 13848 - 13855. [Abstract] [Full Text] [PDF]
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