![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 5, 1325-1335, May 1999
and
*Institute for Microbiology and Genetics, Georg-August-University,
D-37077 Göttingen, Germany; and The two highly conserved RAS genes of the budding
yeast Saccharomyces cerevisiae are redundant for
viability. Here we show that haploid invasive growth development
depends on RAS2 but not RAS1. Ras1p is
not sufficiently expressed to induce invasive growth. Ras2p activates
invasive growth using either of two downstream signaling pathways, the
filamentation MAPK (Cdc42p/Ste20p/MAPK) cascade or the
cAMP-dependent protein kinase (Cyr1p/cAMP/PKA) pathway. This signal
branch point can be uncoupled in cells expressing Ras2p mutant proteins
that carry amino acid substitutions in the adenylyl cyclase interaction
domain and therefore activate invasive growth solely dependent on the
MAPK cascade. Both Ras2p-controlled signaling pathways stimulate
expression of the filamentation response element-driven reporter gene
depending on the transcription factors Ste12p and Tec1p, indicating a
crosstalk between the MAPK and the cAMP signaling pathways in haploid
cells during invasive growth.
Whitehead Institute
for Biomedical Research, Cambridge, Massachusetts 02142
Corresponding author: E-mail address:
gbraus{at}gwdg.de.
This article has been cited by other articles:
|
|
 |
|
  S. Maerz, C. Ziv, N. Vogt, K. Helmstaedt, N. Cohen, R. Gorovits, O. Yarden, and S. Seiler The Nuclear Dbf2-Related Kinase COT1 and the Mitogen-Activated Protein Kinases MAK1 and MAK2 Genetically Interact to Regulate Filamentous Growth, Hyphal Fusion and Sexual Development in Neurospora crassa Genetics, July 1, 2008; 179(3): 1313 - 1325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Valerius, M. Kleinschmidt, N. Rachfall, F. Schulze, S. Lopez Marin, M. Hoppert, K. Streckfuss-Bomeke, C. Fischer, and G. H. Braus The Saccharomyces Homolog of Mammalian RACK1, Cpc2/Asc1p, Is Required for FLO11-dependent Adhesive Growth and Dimorphism Mol. Cell. Proteomics, November 1, 2007; 6(11): 1968 - 1979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Zeller, S. C. Parnell, and H. G. Dohlman The RACK1 Ortholog Asc1 Functions as a G-protein beta Subunit Coupled to Glucose Responsiveness in Yeast J. Biol. Chem., August 24, 2007; 282(34): 25168 - 25176. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-O. Park and E. Bi Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 48 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. B. Reynolds The Opi1p Transcription Factor Affects Expression of FLO11, Mat Formation, and Invasive Growth in Saccharomyces cerevisiae. Eukaryot. Cell, August 1, 2006; 5(8): 1266 - 1275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chou, S. Lane, and H. Liu Regulation of Mating and Filamentation Genes by Two Distinct Ste12 Complexes in Saccharomyces cerevisiae. Mol. Cell. Biol., July 1, 2006; 26(13): 4794 - 4805. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Schubert, M. Raudaskoski, N. Knabe, and E. Kothe Ras GTPase-Activating Protein Gap1 of the Homobasidiomycete Schizophyllum commune Regulates Hyphal Growth Orientation and Sexual Development. Eukaryot. Cell, April 1, 2006; 5(4): 683 - 695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Santangelo Glucose Signaling in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 253 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. S. Felipe, R. V. Andrade, F. B. M. Arraes, A. M. Nicola, A. Q. Maranhao, F. A. G. Torres, I. Silva-Pereira, M. J. Pocas-Fonseca, E. G. Campos, L. M. P. Moraes, et al. Transcriptional Profiles of the Human Pathogenic Fungus Paracoccidioides brasiliensis in Mycelium and Yeast Cells J. Biol. Chem., July 1, 2005; 280(26): 24706 - 24714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. van Dyk, I. S. Pretorius, and F. F. Bauer Mss11p Is a Central Element of the Regulatory Network That Controls FLO11 Expression and Invasive Growth in Saccharomyces cerevisiae Genetics, January 1, 2005; 169(1): 91 - 106. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-S. Bahn, J. K. Hicks, S. S. Giles, G. M. Cox, and J. Heitman Adenylyl Cyclase-Associated Protein Aca1 Regulates Virulence and Differentiation of Cryptococcus neoformans via the Cyclic AMP-Protein Kinase A Cascade Eukaryot. Cell, December 1, 2004; 3(6): 1476 - 1491. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. K. SINGH, A. K. RASMUSSEN, and L. J. RASMUSSEN Genome-Wide Analysis of Signal Transducers and Regulators of Mitochondrial Dysfunction in Saccharomyces cerevisiae Ann. N.Y. Acad. Sci., April 1, 2004; 1011(1): 284 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. R. Steen, S. Zuyderduyn, D. L. Toffaletti, M. Marra, S. J. M. Jones, J. R. Perfect, and J. Kronstad Cryptococcus neoformans Gene Expression during Experimental Cryptococcal Meningitis Eukaryot. Cell, December 1, 2003; 2(6): 1336 - 1349. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G. Kozminski, L. Beven, E. Angerman, A. H. Y. Tong, C. Boone, and H.-O. Park Interaction between a Ras and a Rho GTPase Couples Selection of a Growth Site to the Development of Cell Polarity in Yeast Mol. Biol. Cell, December 1, 2003; 14(12): 4958 - 4970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. H. Braus, O. Grundmann, S. Bruckner, and H.-U. Mosch Amino Acid Starvation and Gcn4p Regulate Adhesive Growth and FLO11 Gene Expression in Saccharomyces cerevisiae Mol. Biol. Cell, October 1, 2003; 14(10): 4272 - 4284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Sobering, M. J. Romeo, H. A. Vay, and D. E. Levin A Novel Ras Inhibitor, Eri1, Engages Yeast Ras at the Endoplasmic Reticulum Mol. Cell. Biol., July 15, 2003; 23(14): 4983 - 4990. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Honigberg and K. Purnapatre Signal pathway integration in the switch from the mitotic cell cycle to meiosis in yeast J. Cell Sci., June 1, 2003; 116(11): 2137 - 2147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bolte, P. Dieckhoff, C. Krause, G. H. Braus, and S. Irniger Synergistic inhibition of APC/C by glucose and activated Ras proteins can be mediated by each of the Tpk1-3 proteins in Saccharomyces cerevisiae Microbiology, May 1, 2003; 149(5): 1205 - 1216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. G. Toby, W. Gherraby, T. R. Coleman, and E. A. Golemis A Novel RING Finger Protein, Human Enhancer of Invasion 10, Alters Mitotic Progression through Regulation of Cyclin B Levels Mol. Cell. Biol., March 15, 2003; 23(6): 2109 - 2122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Lee and J. W. Kronstad ras2 Controls Morphogenesis, Pheromone Response, and Pathogenicity in the Fungal Pathogen Ustilago maydis Eukaryot. Cell, December 1, 2002; 1(6): 954 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kohler, S. Wesche, N. Taheri, G. H. Braus, and H.-U. Mosch Dual Role of the Saccharomyces cerevisiae TEA/ATTS Family Transcription Factor Tec1p in Regulation of Gene Expression and Cellular Development Eukaryot. Cell, October 1, 2002; 1(5): 673 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Calvo, R. A. Wilson, J. W. Bok, and N. P. Keller Relationship between Secondary Metabolism and Fungal Development Microbiol. Mol. Biol. Rev., September 1, 2002; 66(3): 447 - 459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Palecek, A. S. Parikh, and S. J. Kron Sensing, signalling and integrating physical processes during Saccharomyces cerevisiae invasive and filamentous growth Microbiology, April 1, 2002; 148(4): 893 - 907. [Full Text] [PDF]
|
|
|
|
|
|
M. S. Waugh, C. B. Nichols, C. M. DeCesare, G. M. Cox, J. Heitman, and J. A. Alspaugh Ras1 and Ras2 contribute shared and unique roles in physiology and virulence of Cryptococcus neoformans Microbiology, January 1, 2002; 148(1): 191 - 201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. S. Cutler, X. Pan, J. Heitman, and M. E. Cardenas The TOR Signal Transduction Cascade Controls Cellular Differentiation in Response to Nutrients Mol. Biol. Cell, December 1, 2001; 12(12): 4103 - 4113. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Scherz, V. Shinder, and D. Engelberg Anatomical Analysis of Saccharomyces cerevisiae Stalk-Like Structures Reveals Spatial Organization and Cell Specialization J. Bacteriol., September 15, 2001; 183(18): 5402 - 5413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Miled, C. Mann, and G. Faye Xbp1-Mediated Repression of CLB Gene Expression Contributes to the Modifications of Yeast Cell Morphology and Cell Cycle Seen during Nitrogen-Limited Growth Mol. Cell. Biol., June 1, 2001; 21(11): 3714 - 3724. [Abstract] [Full Text]
|
|
|
|
|
|
J. Ho and A. Bretscher Ras Regulates the Polarity of the Yeast Actin Cytoskeleton through the Stress Response Pathway Mol. Biol. Cell, June 1, 2001; 12(6): 1541 - 1555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Richard, R. R. Quijano, S. Bezzate, F. Bordon-Pallier, and C. Gaillardin Tagging Morphogenetic Genes by Insertional Mutagenesis in the Yeast Yarrowia lipolytica J. Bacteriol., May 15, 2001; 183(10): 3098 - 3107. [Abstract] [Full Text]
|
|
|
|
|
|
Y.-S. Bahn and P. Sundstrom CAP1, an Adenylate Cyclase-Associated Protein Gene, Regulates Bud-Hypha Transitions, Filamentous Growth, and Cyclic AMP Levels and Is Required for Virulence of Candida albicans J. Bacteriol., May 15, 2001; 183(10): 3211 - 3223. [Abstract] [Full Text]
|
|
|
|
|
|
H.-U. Mösch, T. Köhler, and G. H. Braus Different Domains of the Essential GTPase Cdc42p Required for Growth and Development of Saccharomyces cerevisiae Mol. Cell. Biol., January 1, 2001; 21(1): 235 - 248. [Abstract] [Full Text]
|
|
|
|
|
|
K. B. Lengeler, R. C. Davidson, C. D'souza, T. Harashima, W.-C. Shen, P. Wang, X. Pan, M. Waugh, and J. Heitman Signal Transduction Cascades Regulating Fungal Development and Virulence Microbiol. Mol. Biol. Rev., December 1, 2000; 64(4): 746 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Palecek, A. S. Parikh, and S. J. Kron Genetic Analysis Reveals That FLO11 Upregulation and Cell Polarization Independently Regulate Invasive Growth in Saccharomyces cerevisiae Genetics, November 1, 2000; 156(3): 1005 - 1023. [Abstract] [Full Text]
|
|
|
|
|
|
G. C. Tomlin, G. E. Hamilton, D. C. J. Gardner, R. M. Walmsley, L. I. Stateva, and S. G. Oliver Suppression of sorbitol dependence in a strain bearing a mutation in the SRB1/PSA1/VIG9 gene encoding GDP-mannose pyrophosphorylase by PDE2 overexpression suggests a role for the Ras/cAMP signal-transduction pathway in the control of yeast cell-wall biogenesis Microbiology, September 1, 2000; 146(9): 2133 - 2146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Irniger, M. Bäumer, and G. H. Braus Glucose and Ras Activity Influence the Ubiquitin Ligases APC/C and SCF in Saccharomyces cerevisiae Genetics, April 1, 2000; 154(4): 1509 - 1521. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. J. Roberts, B. Nelson, M. J. Marton, R. Stoughton, M. R. Meyer, H. A. Bennett, Y. D. He, H. Dai, W. L. Walker, T. R. Hughes, et al. Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles Science, February 4, 2000; 287(5454): 873 - 880. [Abstract] [Full Text]
|
|
|
|
|
|
M. C. Lorenz, X. Pan, T. Harashima, M. E. Cardenas, Y. Xue, J. P. Hirsch, and J. Heitman The G Protein-Coupled Receptor Gpr1 Is a Nutrient Sensor That Regulates Pseudohyphal Differentiation in Saccharomyces cerevisiae Genetics, February 1, 2000; 154(2): 609 - 622. [Abstract] [Full Text]
|
|
|
|
|
|
K. Ansari, S. Martin, M. Farkasovsky, I.-M. Ehbrecht, and H. Kuntzel Phospholipase C Binds to the Receptor-like GPR1 Protein and Controls Pseudohyphal Differentiation in Saccharomyces cerevisiae J. Biol. Chem., October 15, 1999; 274(42): 30052 - 30058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Ahn, A. Acurio, and S. J. Kron Regulation of G2/M Progression by the STE Mitogen-activated Protein Kinase Pathway in Budding Yeast Filamentous Growth Mol. Biol. Cell, October 1, 1999; 10(10): 3301 - 3316. [Abstract] [Full Text]
|
|
|
|
|
|
O. Grundmann, H.-U. Mosch, and G. H. Braus Repression of GCN4 mRNA Translation by Nitrogen Starvation in Saccharomyces cerevisiae J. Biol. Chem., July 6, 2001; 276(28): 25661 - 25671. [Abstract] [Full Text] [PDF]
|
|
