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Vol. 10, Issue 5, 1477-1494, May 1999
Morphogenèse et Signalisation Cellulaires, Centre National de
la Recherche Scientifique, Unité Mixte de Recherche 144, Institut Curie, 75248 Paris Cedex 05, France
Myosin Is, which constitute a ubiquitous monomeric subclass of
myosins with actin-based motor properties, are associated with plasma
membrane and intracellular vesicles. Myosin Is have been proposed as
key players for membrane trafficking in endocytosis or exocytosis. In
the present paper we provide biochemical and immunoelectron microscopic
evidence indicating that a pool of myosin I alpha (MMI
) is
associated with endosomes and lysosomes. We show that the
overproduction of MMI or the production of nonfunctional truncated
MMI affects the distribution of the endocytic compartments. We also
show that truncated brush border myosin I proteins, myosin Is
that share 78% homology with MMI, promote the dissociation of
MMI from vesicular membranes derived from endocytic compartments. The analysis at the ultrastructural level of cells producing these brush border myosin I truncated proteins shows that the delivery of the
fluid phase markers from endosomes to lysosomes is impaired. MMI
might therefore be involved in membrane trafficking occurring between
endosomes and lysosomes.
Corresponding author. E-mail address:
coudrier{at}curie.fr.
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