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Vol. 10, Issue 5, 1665-1683, May 1999
*Department of Microbiology and Immunology, Astrocytes in neuron-free cultures typically lack processes,
although they are highly process-bearing in vivo. We show that basic
fibroblast growth factor (bFGF) induces cultured astrocytes to grow
processes and that Ras family GTPases mediate these morphological changes. Activated alleles of rac1 and
rhoA blocked and reversed bFGF effects when introduced
into astrocytes in dissociated culture and in brain slices using
recombinant adenoviruses. By contrast, dominant negative (DN) alleles
of both GTPases mimicked bFGF effects. A DN allele of
Ha-ras blocked bFGF effects but not those of Rac1-DN or
RhoA-DN. Our results show that bFGF acting through c-Ha-Ras inhibits
endogenous Rac1 and RhoA GTPases thereby triggering astrocyte process
growth, and they provide evidence for the regulation of this cascade in
vivo by a yet undetermined neuron-derived factor.
Neuroscience Graduate
Program, University of California at San Francisco, San Francisco,
California 94143; and §Cell Genesys Inc., Foster City,
California 94404
Corresponding author. E-mail address:
kalman{at}cgl.ucsf.edu.
Molecular Biology of the Cell
Vol. 10, 1665-1683, May 1999
Copyright © 1999 by The American Society for Cell Biology
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