![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 6, 1719-1732, June 1999
Institute of Molecular Biology, University of Oregon, Eugene,
Oregon 97403-1229
The interaction between v-SNAREs on transport vesicles and t-SNAREs
on target membranes is required for membrane traffic in eukaryotic
cells. Here we identify Vti1p as the first v-SNARE protein found to be
required for biosynthetic traffic into the yeast vacuole, the
equivalent of the mammalian lysosome. Certain vti1-ts
yeast mutants are defective in alkaline phosphatase transport from the
Golgi to the vacuole and in targeting of aminopeptidase I from the cytosol to the vacuole. VTI1 interacts
genetically with the vacuolar t-SNARE VAM3, which is
required for transport of both alkaline phosphatase and
aminopeptidase I to the vacuole. The v-SNARE Nyv1p
forms a SNARE complex with Vam3p in homotypic vacuolar fusion; however,
we find that Nyv1p is not required for any of the three biosynthetic
pathways to the vacuole. v-SNAREs were thought to ensure specificity in
membrane traffic. However, Vti1p also functions in two additional
membrane traffic pathways: Vti1p interacts with the t-SNAREs Pep12p in
traffic from the TGN to the prevacuolar compartment and with Sed5p in
retrograde traffic to the cis-Golgi. The ability of
Vti1p to mediate multiple fusion steps requires additional proteins to
ensure specificity in membrane traffic.
Corresponding author. E-mail address:
stevens{at}molbio.uoregon.edu.
This article has been cited by other articles:
|
|
 |
|
  S. Chidambaram, J. Zimmermann, and G. F. von Mollard ENTH domain proteins are cargo adaptors for multiple SNARE proteins at the TGN endosome J. Cell Sci., February 1, 2008; 121(3): 329 - 338. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Wiradjaja, L. M. Ooms, S. Tahirovic, E. Kuhne, R. J. Devenish, A. L. Munn, R. C. Piper, P. Mayinger, and C. A. Mitchell Inactivation of the Phosphoinositide Phosphatases Sac1p and Inp54p Leads to Accumulation of Phosphatidylinositol 4,5-Bisphosphate on Vacuole Membranes and Vacuolar Fusion Defects J. Biol. Chem., June 1, 2007; 282(22): 16295 - 16307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sanmartin, A. Ordonez, E. J. Sohn, S. Robert, J. J. Sanchez-Serrano, M. A. Surpin, N. V. Raikhel, and E. Rojo Divergent functions of VTI12 and VTI11 in trafficking to storage and lytic vacuoles in Arabidopsis PNAS, February 27, 2007; 104(9): 3645 - 3650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Kama, M. Robinson, and J. E. Gerst Btn2, a Hook1 Ortholog and Potential Batten Disease-Related Protein, Mediates Late Endosome-Golgi Protein Sorting in Yeast Mol. Cell. Biol., January 15, 2007; 27(2): 605 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. Graf, D. Riedel, H. D. Schmitt, and R. Jahn Identification of Functionally Interacting SNAREs by Using Complementary Substitutions in the Conserved `0' Layer Mol. Biol. Cell, May 1, 2005; 16(5): 2263 - 2274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. LaGrassa and C. Ungermann The vacuolar kinase Yck3 maintains organelle fragmentation by regulating the HOPS tethering complex J. Cell Biol., January 31, 2005; 168(3): 401 - 414. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Klionsky The molecular machinery of autophagy: unanswered questions J. Cell Sci., January 1, 2005; 118(1): 7 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chidambaram, N. Mullers, K. Wiederhold, V. Haucke, and G. F. von Mollard Specific Interaction between SNAREs and Epsin N-terminal Homology (ENTH) Domains of Epsin-related Proteins in trans-Golgi Network to Endosome Transport J. Biol. Chem., February 6, 2004; 279(6): 4175 - 4179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Surpin, H. Zheng, M. T. Morita, C. Saito, E. Avila, J. J. Blakeslee, A. Bandyopadhyay, V. Kovaleva, D. Carter, A. Murphy, et al. The VTI Family of SNARE Proteins Is Necessary for Plant Viability and Mediates Different Protein Transport Pathways PLANT CELL, December 1, 2003; 15(12): 2885 - 2899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Atlashkin, V. Kreykenbohm, E.-L. Eskelinen, D. Wenzel, A. Fayyazi, and G. Fischer von Mollard Deletion of the SNARE vti1b in Mice Results in the Loss of a Single SNARE Partner, Syntaxin 8 Mol. Cell. Biol., August 1, 2003; 23(15): 5198 - 5207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Duman and J. G. Forte What is the role of SNARE proteins in membrane fusion? Am J Physiol Cell Physiol, August 1, 2003; 285(2): C237 - C249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. R. Brown, J. Liu, G.-C. Hung, D. Carter, D. Cui, and H.-L. Chiang The Vid Vesicle to Vacuole Trafficking Event Requires Components of the SNARE Membrane Fusion Machinery J. Biol. Chem., July 3, 2003; 278(28): 25688 - 25699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kweon, A. Rothe, E. Conibear, and T. H. Stevens Ykt6p Is a Multifunctional Yeast R-SNARE That Is Required for Multiple Membrane Transport Pathways to the Vacuole Mol. Biol. Cell, May 1, 2003; 14(5): 1868 - 1881. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-W. Wang, P. E. Stromhaug, J. Shima, and D. J. Klionsky The Ccz1-Mon1 Protein Complex Is Required for the Late Step of Multiple Vacuole Delivery Pathways J. Biol. Chem., November 27, 2002; 277(49): 47917 - 47927. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Xue and B. Zhang Do SNARE proteins confer specificity for vesicle fusion? PNAS, October 15, 2002; 99(21): 13359 - 13361. [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu and C. Barlowe Analysis of Sec22p in Endoplasmic Reticulum/Golgi Transport Reveals Cellular Redundancy in SNARE Protein Function Mol. Biol. Cell, September 1, 2002; 13(9): 3314 - 3324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shorter, M. B. Beard, J. Seemann, A. B. Dirac-Svejstrup, and G. Warren Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115 J. Cell Biol., April 1, 2002; 157(1): 45 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mallard, B. L. Tang, T. Galli, D. Tenza, A. Saint-Pol, X. Yue, C. Antony, W. Hong, B. Goud, and L. Johannes Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform J. Cell Biol., February 18, 2002; 156(4): 653 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Reggiori and D. J. Klionsky Autophagy in the Eukaryotic Cell Eukaryot. Cell, February 1, 2002; 1(1): 11 - 21. [Full Text] [PDF]
|
|
|
|
|
|
D. Geelen, B. Leyman, H. Batoko, G.-P. Di Sansebastiano, I. Moore, and M. R. Blatt The Abscisic Acid-Related SNARE Homolog NtSyr1 Contributes to Secretion and Growth: Evidence from Competition with Its Cytosolic Domain PLANT CELL, February 1, 2002; 14(2): 387 - 406. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ishihara, M. Hamasaki, S. Yokota, K. Suzuki, Y. Kamada, A. Kihara, T. Yoshimori, T. Noda, and Y. Ohsumi Autophagosome Requires Specific Early Sec Proteins for Its Formation and NSF/SNARE for Vacuolar Fusion Mol. Biol. Cell, November 1, 2001; 12(11): 3690 - 3702. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Abeliovich and D. J. Klionsky Autophagy in Yeast: Mechanistic Insights and Physiological Function Microbiol. Mol. Biol. Rev., September 1, 2001; 65(3): 463 - 479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M.K. Tsui, W. C.S. Tai, and D. K. Banfield Selective Formation of Sed5p-containing SNARE Complexes Is Mediated by Combinatorial Binding Interactions Mol. Biol. Cell, March 1, 2001; 12(3): 521 - 538. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. A. Sanderfoot, F. F. Assaad, and N. V. Raikhel The Arabidopsis Genome. An Abundance of Soluble N-Ethylmaleimide-Sensitive Factor Adaptor Protein Receptors Plant Physiology, December 1, 2000; 124(4): 1558 - 1569. [Abstract] [Full Text]
|
|
|
|
|
|
T. Kirisako, Y. Ichimura, H. Okada, Y. Kabeya, N. Mizushima, T. Yoshimori, M. Ohsumi, T. Takao, T. Noda, and Y. Ohsumi The Reversible Modification Regulates the Membrane-Binding State of Apg8/Aut7 Essential for Autophagy and the Cytoplasm to Vacuole Targeting Pathway J. Cell Biol., October 18, 2000; 151(2): 263 - 276. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gurunathan, D. Chapman-Shimshoni, S. Trajkovic, and J. E. Gerst Yeast Exocytic v-SNAREs Confer Endocytosis Mol. Biol. Cell, October 1, 2000; 11(10): 3629 - 3643. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Srivastava, C. A. Woolford, and E. W. Jones Pep3p/Pep5p Complex: A Putative Docking Factor at Multiple Steps of Vesicular Transport to the Vacuole of Saccharomyces cerevisiae Genetics, September 1, 2000; 156(1): 105 - 122. [Abstract] [Full Text]
|
|
|
|
 |
|
 W. Antonin, D. Riedel, and G. F. von Mollard The SNARE Vti1a-beta Is Localized to Small Synaptic Vesicles and Participates in a Novel SNARE Complex J. Neurosci., August 1, 2000; 20(15): 5724 - 5732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Ward, J. Pevsner, M. A. Scullion, M. Vaughn, and J. Kaplan Syntaxin 7 and VAMP-7 are Soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptors Required for Late Endosome-Lysosome and Homotypic Lysosome Fusion in Alveolar Macrophages Mol. Biol. Cell, July 1, 2000; 11(7): 2327 - 2333. [Abstract] [Full Text]
|
|
|
|
|
|
X. Cao and C. Barlowe Asymmetric Requirements for a Rab GTPase and SNARE Proteins in Fusion of COPII Vesicles with Acceptor Membranes J. Cell Biol., April 3, 2000; 149(1): 55 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rubino, M. Miaczynska, R. Lippe, and M. Zerial Selective Membrane Recruitment of EEA1 Suggests a Role in Directional Transport of Clathrin-coated Vesicles to Early Endosomes J. Biol. Chem., February 11, 2000; 275(6): 3745 - 3748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Subramaniam, E Loh, H Horstmann, A Habermann, Y Xu, J Coe, G Griffiths, and W Hong Preferential association of syntaxin 8 with the early endosome J. Cell Sci., January 3, 2000; 113(6): 997 - 1008. [Abstract] [PDF]
|
|
|
|
|
|
M. Tsui and D. Banfield Yeast Golgi SNARE interactions are promiscuous J. Cell Sci., January 1, 2000; 113(1): 145 - 152. [Abstract] [PDF]
|
|
|
|
|
|
E. Conibear and T. H. Stevens Vps52p, Vps53p, and Vps54p Form a Novel Multisubunit Complex Required for Protein Sorting at the Yeast Late Golgi Mol. Biol. Cell, January 1, 2000; 11(1): 305 - 323. [Abstract] [Full Text]
|
|
|
|
|
|
S. M. VanRheenen, X. Cao, S. K. Sapperstein, E. C. Chiang, V. V. Lupashin, C. Barlowe, and M. G. Waters Sec34p, a Protein Required for Vesicle Tethering to the Yeast Golgi Apparatus, Is in a Complex with Sec35p J. Cell Biol., November 15, 1999; 147(4): 729 - 742. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Sanderfoot, V. Kovaleva, H. Zheng, and N. V. Raikhel The t-SNARE AtVAM3p Resides on the Prevacuolar Compartment in Arabidopsis Root Cells Plant Physiology, November 1, 1999; 121(3): 929 - 938. [Abstract] [Full Text]
|
|
|
|
|
|
H. Zheng, G. F. von Mollard, V. Kovaleva, T. H. Stevens, and N. V. Raikhel The Plant Vesicle-associated SNARE AtVTI1a Likely Mediates Vesicle Transport from the Trans-Golgi Network to the Prevacuolar Compartment Mol. Biol. Cell, July 1, 1999; 10(7): 2251 - 2264. [Abstract] [Full Text]
|
|
|
|
|
|
C. Ungermann, G. F. von Mollard, O. N. Jensen, N. Margolis, T. H. Stevens, and W. Wickner Three v-SNAREs and Two t-SNAREs, Present in a Pentameric cis-SNARE Complex on Isolated Vacuoles, Are Essential for Homotypic Fusion J. Cell Biol., June 28, 1999; 145(7): 1435 - 1442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Legesse-Miller, Y. Sagiv, R. Glozman, and Z. Elazar Aut7p, a Soluble Autophagic Factor, Participates in Multiple Membrane Trafficking Processes J. Biol. Chem., October 13, 2000; 275(42): 32966 - 32973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takita, L. Engstrom, C. Ungermann, and K. W. Cunningham Inhibition of the Ca2+-ATPase Pmc1p by the v-SNARE Protein Nyv1p J. Biol. Chem., February 23, 2001; 276(9): 6200 - 6206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Wade, N. J. Bryant, L. M. Connolly, R. J. Simpson, J. P. Luzio, R. C. Piper, and D. E. James Syntaxin 7 Complexes with Mouse Vps10p Tail Interactor 1b, Syntaxin 6, Vesicle-associated Membrane Protein (VAMP)8, and VAMP7 in B16 Melanoma Cells J. Biol. Chem., June 1, 2001; 276(23): 19820 - 19827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Dilcher, B. Kohler, and G. F. von Mollard Genetic Interactions with the Yeast Q-SNARE VTI1 Reveal Novel Functions for the R-SNARE YKT6 J. Biol. Chem., September 7, 2001; 276(37): 34537 - 34544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, I. Dulubova, J. Rizo, and T. C. Sudhof Functional Analysis of Conserved Structural Elements in Yeast Syntaxin Vam3p J. Biol. Chem., July 20, 2001; 276(30): 28598 - 28605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Mallard, B. L. Tang, T. Galli, D. Tenza, A. Saint-Pol, X. Yue, C. Antony, W. Hong, B. Goud, and L. Johannes Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform J. Cell Biol., February 18, 2002; 156(4): 653 - 664. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shorter, M. B. Beard, J. Seemann, A. B. Dirac-Svejstrup, and G. Warren Sequential tethering of Golgins and catalysis of SNAREpin assembly by the vesicle-tethering protein p115 J. Cell Biol., April 1, 2002; 157(1): 45 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kato, M. T. Morita, H. Fukaki, Y. Yamauchi, M. Uehara, M. Niihama, and M. Tasaka SGR2, a Phospholipase-Like Protein, and ZIG/SGR4, a SNARE, Are Involved in the Shoot Gravitropism of Arabidopsis PLANT CELL, January 1, 2002; 14(1): 33 - 46. [Abstract] [Full Text] [PDF]
|
|
