![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 6, 1745-1761, June 1999
*Department of Biology, University of California, San Diego, La
Jolla, California 92093-0322; and §Laboratory of Electron
Microscopy, Genentech, South San Francisco, California 94080
We report the cloning and characterization of Pichia
pastoris PEX19 by complementation of a peroxisome-deficient
mutant strain. Import of peroxisomal targeting signal 1- and
2-containing peroxisomal matrix proteins is defective in
pex19 mutants. PEX19 encodes a hydrophilic 299-amino acid protein with sequence similarity to Saccharomyces cerevisiae Pex19p and human and Chinese
hamster PxF, all farnesylated proteins, as well as hypothetical
proteins from Caenorhabditis elegans and
Schizosaccharomyces pombe. The farnesylation consensus
is conserved in PpPex19p but dispensable for function and appears
unmodified under the conditions tested. Pex19p localizes predominantly
to the cytosolic fraction. Biochemical and two-hybrid analyses
confirmed that Pex19p interacts with Pex3p, as seen in S.
cerevisiae, but unexpectedly also with Pex10p. Two-hybrid analysis demonstrated that the amino-terminal 42 amino acids of Pex19p
interact with the carboxyl-terminal 335 amino acids of Pex3p. In
addition, the extreme carboxyl terminus of Pex19p (67 amino acids) is
required for interaction with the amino-terminal 380 amino acids of
Pex10p. Biochemical and immunofluorescence microscopy analyses of
pex19
cells identified the membrane protein Pex3p in
peroxisome remnants that were not previously observed in S.
cerevisiae. These small vesicular and tubular (early) remnants are morphologically distinct from other Pppex mutant
(late) remnants, suggesting that Pex19p functions at an early stage of
peroxisome biogenesis.
These authors contributed equally.
Present addresses: (K.N.F.) University of
Groningen, Department of Microbiology, 9751 NN Haren, The Netherlands;
(T.J.W.) Gist-Brocades, Food Specialities Division, 2600 MA Delft, The Netherlands; (G.H.L.) University of Bonn, Institute for Anatomy, 53115 Bonn, Germany.
Corresponding author: E-mail address:
ssubramani{at}ucsd.edu.
This article has been cited by other articles:
|
|
 |
|
  M. Yan, D. A. Rachubinski, S. Joshi, R. A. Rachubinski, and S. Subramani Dysferlin Domain-containing Proteins, Pex30p and Pex31p, Localized to Two Compartments, Control the Number and Size of Oleate-induced Peroxisomes in Pichia pastoris Mol. Biol. Cell, March 1, 2008; 19(3): 885 - 898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Woolfit, E. Rozpedowska, J. Piskur, and K. H. Wolfe Genome Survey Sequencing of the Wine Spoilage Yeast Dekkera (Brettanomyces) bruxellensis Eukaryot. Cell, April 1, 2007; 6(4): 721 - 733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Lockshon, L. E. Surface, E. O. Kerr, M. Kaeberlein, and B. K. Kennedy The Sensitivity of Yeast Mutants to Oleic Acid Implicates the Peroxisome and Other Processes in Membrane Function Genetics, January 1, 2007; 175(1): 77 - 91. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Matsuzono, T. Matsuzaki, and Y. Fujiki Functional domain mapping of peroxin Pex19p: interaction with Pex3p is essential for function and translocation J. Cell Sci., September 1, 2006; 119(17): 3539 - 3550. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Navarro, M. Russo, V. Pantaleo, and L. Rubino Cytological analysis of Saccharomyces cerevisiae cells supporting cymbidium ringspot virus defective interfering RNA replication. J. Gen. Virol., March 1, 2006; 87(Pt 3): 705 - 714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhang, S. Leon, and S. Subramani Two Independent Pathways Traffic the Intraperoxisomal Peroxin PpPex8p into Peroxisomes: Mechanism and Evolutionary Implications Mol. Biol. Cell, February 1, 2006; 17(2): 690 - 699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Matsuzono and Y. Fujiki In Vitro Transport of Membrane Proteins to Peroxisomes by Shuttling Receptor Pex19p J. Biol. Chem., January 6, 2006; 281(1): 36 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Kragt, T. Voorn-Brouwer, M. van den Berg, and B. Distel Endoplasmic Reticulum-directed Pex3p Routes to Peroxisomes and Restores Peroxisome Formation in a Saccharomyces cerevisiae pex3{Delta} Strain J. Biol. Chem., October 7, 2005; 280(40): 34350 - 34357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shibata, Y. Kashiwayama, T. Imanaka, and H. Kato Domain Architecture and Activity of Human Pex19p, a Chaperone-like Protein for Intracellular Trafficking of Peroxisomal Membrane Proteins J. Biol. Chem., September 10, 2004; 279(37): 38486 - 38494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Otzen, U. Perband, D. Wang, R. J. S. Baerends, W. H. Kunau, M. Veenhuis, and I. J. Van der Klei Hansenula polymorpha Pex19p Is Essential for the Formation of Functional Peroxisomal Membranes J. Biol. Chem., April 30, 2004; 279(18): 19181 - 19190. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Guo, Y. Y. Kit, J.-M. Nicaud, M.-T. Le Dall, S. K. Sears, H. Vali, H. Chan, R. A. Rachubinski, and V. I. Titorenko Peroxisome division in the yeast Yarrowia lipolytica is regulated by a signal from inside the peroxisome J. Cell Biol., September 29, 2003; 162(7): 1255 - 1266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Eckert and N. Johnsson Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome J. Cell Sci., September 1, 2003; 116(17): 3623 - 3634. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Schumann, G. Wanner, M. Veenhuis, M. Schmid, and C. Gietl AthPEX10, a nuclear gene essential for peroxisome and storage organelle formation during Arabidopsis embryogenesis PNAS, August 5, 2003; 100(16): 9626 - 9631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Honsho, T. Hiroshige, and Y. Fujiki The Membrane Biogenesis Peroxin Pex16p. TOPOGENESIS AND FUNCTIONAL ROLES IN PEROXISOMAL MEMBRANE ASSEMBLY J. Biol. Chem., November 8, 2002; 277(46): 44513 - 44524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wadhwa, T. Sugihara, Md. K. Hasan, K. Taira, R. R. Reddel, and S. C. Kaul A Major Functional Difference between the Mouse and Human ARF Tumor Suppressor Proteins J. Biol. Chem., September 20, 2002; 277(39): 36665 - 36670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Harper, S. T. South, J. M. McCaffery, and S. J. Gould Peroxisomal Membrane Protein Import Does Not Require Pex17p J. Biol. Chem., May 3, 2002; 277(19): 16498 - 16504. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Brosius, T. Dehmel, and J. Gartner Two Different Targeting Signals Direct Human Peroxisomal Membrane Protein 22 to Peroxisomes J. Biol. Chem., January 4, 2002; 277(1): 774 - 784. [Abstract] [Full Text]
|
|
|
|
|
|
P. E. Purdue and P. B. Lazarow Pex18p Is Constitutively Degraded during Peroxisome Biogenesis J. Biol. Chem., December 7, 2001; 276(50): 47684 - 47689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Lambkin and R. A. Rachubinski Yarrowia lipolytica Cells Mutant for the Peroxisomal Peroxin Pex19p Contain Structures Resembling Wild-Type Peroxisomes Mol. Biol. Cell, November 1, 2001; 12(11): 3353 - 3364. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fransen, T. Wylin, C. Brees, G. P. Mannaerts, and P. P. Van Veldhoven Human Pex19p Binds Peroxisomal Integral Membrane Proteins at Regions Distinct from Their Sorting Sequences Mol. Cell. Biol., July 1, 2001; 21(13): 4413 - 4424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bottger, P. Barnett, A. T. J. Klein, A. Kragt, H. F. Tabak, and B. Distel Saccharomyces cerevisiae PTS1 Receptor Pex5p Interacts with the SH3 Domain of the Peroxisomal Membrane Protein Pex13p in an Unconventional, Non-PXXP-related Manner Mol. Biol. Cell, November 1, 2000; 11(11): 3963 - 3976. [Abstract] [Full Text]
|
|
|
|
|
|
W. B. Snyder, A. Koller, A. J. Choy, and S. Subramani The Peroxin Pex19p Interacts with Multiple, Integral Membrane Proteins at the Peroxisomal Membrane J. Cell Biol., June 12, 2000; 149(6): 1171 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ghaedi, S. Tamura, K. Okumoto, Y. Matsuzono, and Y. Fujiki The Peroxin Pex3p Initiates Membrane Assembly in Peroxisome Biogenesis Mol. Biol. Cell, June 1, 2000; 11(6): 2085 - 2102. [Abstract] [Full Text]
|
|
|
|
|
|
K. A. Sacksteder, J. M. Jones, S. T. South, X. Li, Y. Liu, and S. J. Gould PEX19 Binds Multiple Peroxisomal Membrane Proteins, Is Predominantly Cytoplasmic, and Is Required for Peroxisome Membrane Synthesis J. Cell Biol., March 6, 2000; 148(5): 931 - 944. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. B. Snyder, A. Koller, A. J. Choy, M. A. Johnson, J. M. Cregg, L. Rangell, G. A. Keller, and S. Subramani Pex17p Is Required for Import of Both Peroxisome Membrane and Lumenal Proteins and Interacts with Pex19p and the Peroxisome Targeting Signal-Receptor Docking Complex in Pichia pastoris Mol. Biol. Cell, December 1, 1999; 10(12): 4005 - 4019. [Abstract] [Full Text]
|
|
|
|
|
|
K. N. Faber, A. M. Kram, M. Ehrmann, and M. Veenhuis A Novel Method to Determine the Topology of Peroxisomal Membrane Proteins in Vivo Using the Tobacco Etch Virus Protease J. Biol. Chem., September 21, 2001; 276(39): 36501 - 36507. [Abstract] [Full Text] [PDF]
|
|
