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Vol. 10, Issue 6, 1859-1872, June 1999
Departments of *Biochemistry and ¶Anatomy
and Embryology, University of Amsterdam, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands; §European Molecular Biology
Laboratory, Biochemical Instrumentation Programme, D-69117 Heidelberg,
Germany; We describe a genome-wide characterization of mRNA
transcript levels in yeast grown on the fatty acid oleate, determined
using Serial Analysis of Gene Expression (SAGE). Comparison of this SAGE library with that reported for glucose grown cells revealed the dramatic adaptive response of yeast to a change in carbon source. A
major fraction (>20%) of the 15,000 mRNA molecules in a yeast cell
comprised differentially expressed transcripts, which were derived from
only 2% of the total number of ~6300 yeast genes. Most of the mRNAs
that were differentially expressed code for enzymes or for other
proteins participating in metabolism (e.g., metabolite transporters).
In oleate-grown cells, this was exemplified by the huge increase of
mRNAs encoding the peroxisomal Munich Information Centre for Protein
Sequences, Max-Planck-Institut für Biochemie, D-82152
Martinsried, Germany; and #Unité de
Génétique Moleculaire des Levures, Institut Pasteur,
F-75724 Paris Cedex 15, France
-oxidation enzymes required for
degradation of fatty acids. The data provide evidence for the existence
of redox shuttles across organellar membranes that involve peroxisomal,
cytoplasmic, and mitochondrial enzymes. We also analyzed the mRNA
profile of a mutant strain with deletions of the PIP2
and OAF1 genes, encoding transcription factors required
for induction of genes encoding peroxisomal proteins. Induction of
genes under the immediate control of these factors was abolished; other
genes were up-regulated, indicating an adaptive response to the changed
metabolism imposed by the genetic impairment. We describe a statistical
method for analysis of data obtained by SAGE.
Online version of this article contains a complete data
set. Online version available at www.molbiolcell.org.
Present address: Gene Expression Control
Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields,
London WC2A 3PX, United Kingdom.
Present address: Introgene BV, 2333 AL
Leiden, The Netherlands.
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