![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 6, 1939-1955, June 1999
3):
Complex Formation with Other p24 Family Members
*Cell Biology and Cell Biophysics Program, European
Molecular Biology Laboratory, 69117 Heidelberg, Germany;
We report here the characterization of gp27 (hp24Department of Anatomy, Miyazaki Medical College, Miyazaki
889-1692, Japan; Membrane Biology Laboratory, Institute
of Molecular and Cell Biology, Singapore 117609, Republic of Singapore;
and §Department of Biochemistry, Virginia Polytechnic
Institute and State University, Blacksburg, Virginia 24061-0308
3),
a glycoprotein of the p24 family of small and abundant transmembrane proteins of the secretory pathway. Immunoelectron and confocal scanning
microscopy show that at steady state, gp27 localizes to the
cis side of the Golgi apparatus. In addition, some gp27 was detected in COPI- and COPII-coated structures throughout the cytoplasm. This indicated cycling that was confirmed in three ways.
First, 15°C temperature treatment resulted in accumulation of gp27 in
pre-Golgi structures colocalizing with anterograde cargo. Second,
treatment with brefeldin A caused gp27 to relocate into peripheral
structures positive for both KDEL receptor and COPII. Third,
microinjection of a dominant negative mutant of Sar1p trapped gp27 in
the endoplasmic reticulum (ER) by blocking ER export. Together, this
shows that gp27 cycles extensively in the early secretory pathway.
Immunoprecipitation and coexpression studies further revealed that a
significant fraction of gp27 existed in a hetero-oligomeric complex.
Three members of the p24 family, GMP25 (hp24
2), p24
(hp24
1), and p23 (hp24
1), coprecipitated in what appeared to be stochiometric amounts. This heterocomplex was
specific. Immunoprecipitation of p26 (hp244) failed to
coprecipitate GMP25, p24, or p23. Also, very little p26 was found
coprecipitating with gp27. A functional requirement for complex
formation was suggested at the level of ER export. Transiently
expressed gp27 failed to leave the ER unless other p24 family proteins
were coexpressed. Comparison of attached oligosaccharides showed that
gp27 and GMP25 recycled differentially. Only a very minor portion of
GMP25 displayed complex oligosaccharides. In contrast, all of gp27
showed modifications by medial and trans enzymes at
steady state. We conclude from these data that a portion of gp27 exists
as hetero-oligomeric complexes with GMP25, p24, and p23 and that these
complexes are in dynamic equilibrium with individual p24 proteins to
allow for differential recycling and distributions.
Corresponding author. E-mail address:
nilsson{at}embl-heidelberg.de.
This article has been cited by other articles:
|
|
 |
|
  S. Mitrovic, H. Ben-Tekaya, E. Koegler, J. Gruenberg, and H.-P. Hauri The Cargo Receptors Surf4, Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC)-53, and p25 Are Required to Maintain the Architecture of ERGIC and Golgi Mol. Biol. Cell, May 1, 2008; 19(5): 1976 - 1990. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Aguilera-Romero, J. Kaminska, A. Spang, H. Riezman, and M. Muniz The yeast p24 complex is required for the formation of COPI retrograde transport vesicles from the Golgi apparatus J. Cell Biol., February 25, 2008; 180(4): 713 - 720. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Moelleken, J. Malsam, M. J. Betts, A. Movafeghi, I. Reckmann, I. Meissner, A. Hellwig, R. B. Russell, T. Sollner, B. Brugger, et al. Differential localization of coatomer complex isoforms within the Golgi apparatus PNAS, March 13, 2007; 104(11): 4425 - 4430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hosaka, T. Watanabe, Y. Yamauchi, Y. Sakai, M. Suda, S. Mizutani, T. Takeuchi, T. Isobe, and T. Izumi A Subset of p23 Localized on Secretory Granules in Pancreatic {beta}-cells J. Histochem. Cytochem., March 1, 2007; 55(3): 235 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Gupta and G. Swarup Evidence for a role of transmembrane protein p25 in localization of protein tyrosine phosphatase TC48 to the ER J. Cell Sci., May 1, 2006; 119(9): 1703 - 1714. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Simpson, T. Nilsson, and R. Pepperkok Biogenesis of Tubular ER-to-Golgi Transport Intermediates Mol. Biol. Cell, February 1, 2006; 17(2): 723 - 737. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Young, T. Stauber, E. del Nery, I. Vernos, R. Pepperkok, and T. Nilsson Regulation of Microtubule-dependent Recycling at the Trans-Golgi Network by Rab6A and Rab6A' Mol. Biol. Cell, January 1, 2005; 16(1): 162 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Contreras, Y. Yang, D. G. Robinson, and F. Aniento Sorting Signals in the Cytosolic Tail of Plant p24 Proteins Involved in the Interaction with the COPII Coat Plant Cell Physiol., December 15, 2004; 45(12): 1779 - 1786. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Breuza, R. Halbeisen, P. Jeno, S. Otte, C. Barlowe, W. Hong, and H.-P. Hauri Proteomics of Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC) Membranes from Brefeldin A-treated HepG2 Cells Identifies ERGIC-32, a New Cycling Protein That Interacts with Human Erv46 J. Biol. Chem., November 5, 2004; 279(45): 47242 - 47253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bouw, R. Van Huizen, E. J.R. Jansen, and G. J.M. Martens A Cell-Specific Transgenic Approach in Xenopus Reveals the Importance of a Functional p24 System for a Secretory Cell Mol. Biol. Cell, March 1, 2004; 15(3): 1244 - 1253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Schafer, H. Zentgraf, C. Zehe, B. Brugger, J. Bernhagen, and W. Nickel Unconventional Secretion of Fibroblast Growth Factor 2 Is Mediated by Direct Translocation across the Plasma Membrane of Mammalian Cells J. Biol. Chem., February 20, 2004; 279(8): 6244 - 6251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Emery, R. G. Parton, M. Rojo, and J. Gruenberg The trans-membrane protein p25 forms highly specialized domains that regulate membrane composition and dynamics J. Cell Sci., December 1, 2003; 116(23): 4821 - 4832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Giraudo and H. J.F. Maccioni Endoplasmic Reticulum Export of Glycosyltransferases Depends on Interaction of a Cytoplasmic Dibasic Motif with Sar1 Mol. Biol. Cell, September 1, 2003; 14(9): 3753 - 3766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. E. Carney and B. J. Taylor logjam Encodes a Predicted EMP24/GP25 Protein That Is Required for Drosophila Oviposition Behavior Genetics, May 1, 2003; 164(1): 173 - 186. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Orci, M. Ravazzola, G. J. Mack, C. Barlowe, and S. Otte Mammalian Erv46 localizes to the endoplasmic reticulum-Golgi intermediate compartment and to cis-Golgi cisternae PNAS, April 15, 2003; 100(8): 4586 - 4591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Seelenmeyer, S. Wegehingel, J. Lechner, and W. Nickel The cancer antigen CA125 represents a novel counter receptor for galectin-1 J. Cell Sci., April 1, 2003; 116(7): 1305 - 1318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Jenne, K. Frey, B. Brugger, and F. T. Wieland Oligomeric State and Stoichiometry of p24 Proteins in the Early Secretory Pathway J. Biol. Chem., November 22, 2002; 277(48): 46504 - 46511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Xu, S. Martin, D. E. James, and W. Hong GS15 Forms a SNARE Complex with Syntaxin 5, GS28, and Ykt6 and Is Implicated in Traffic in the Early Cisternae of the Golgi Apparatus Mol. Biol. Cell, October 1, 2002; 13(10): 3493 - 3507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Nickel, B. Brugger, and F. T. Wieland Vesicular transport: the core machinery of COPI recruitment and budding J. Cell Sci., August 15, 2002; 115(16): 3235 - 3240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Sancho, S. Schleich, G. Griffiths, and J. Krijnse-Locker The Block in Assembly of Modified Vaccinia Virus Ankara in HeLa Cells Reveals New Insights into Vaccinia Virus Morphogenesis J. Virol., July 17, 2002; 76(16): 8318 - 8334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rotter, R. P. Kuiper, G. Bouw, and G. J. M. Martens Cell-type-specific and selectively induced expression of members of the p24 family of putative cargo receptors J. Cell Sci., January 3, 2002; 115(5): 1049 - 1058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. A. Barr, C. Preisinger, R. Kopajtich, and R. Korner Golgi matrix proteins interact with p24 cargo receptors and aid their efficient retention in the Golgi apparatus J. Cell Biol., December 10, 2001; 155(6): 885 - 892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Miles, H. McManus, K. E. Forsten, and B. Storrie Evidence that the entire Golgi apparatus cycles in interphase HeLa cells: sensitivity of Golgi matrix proteins to an ER exit block J. Cell Biol., November 12, 2001; 155(4): 543 - 556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. J. Belden and C. Barlowe Distinct Roles for the Cytoplasmic Tail Sequences of Emp24p and Erv25p in Transport between the Endoplasmic Reticulum and Golgi Complex J. Biol. Chem., November 9, 2001; 276(46): 43040 - 43048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Pentcheva and M. Edidin Clustering of Peptide-Loaded MHC Class I Molecules for Endoplasmic Reticulum Export Imaged by Fluorescence Resonance Energy Transfer J. Immunol., June 1, 2001; 166(11): 6625 - 6632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Dahm, J. White, S. Grill, J. Füllekrug, and E. H.K. Stelzer Quantitative ER {left-right-arrow} Golgi Transport Kinetics and Protein Separation upon Golgi Exit Revealed by Vesicular Integral Membrane Protein 36 Dynamics in Live Cells Mol. Biol. Cell, May 1, 2001; 12(5): 1481 - 1498. [Abstract] [Full Text]
|
|
|
|
|
|
J. H. Walenta, A. J. Didier, X. Liu, and H. Kramer The Golgi-associated Hook3 Protein Is a Member of a Novel Family of Microtubule-binding Proteins J. Cell Biol., February 26, 2001; 152(5): 923 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kaiser Thinking about p24 proteins and how transport vesicles select their cargo PNAS, April 11, 2000; 97(8): 3783 - 3785. [Full Text] [PDF]
|
|
|
|
|
|
L. F. Ciufo and A. Boyd Identification of a Lumenal Sequence Specifying the Assembly of Emp24p into p24 Complexes in the Yeast Secretory Pathway J. Biol. Chem., March 17, 2000; 275(12): 8382 - 8388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Muniz, C. Nuoffer, H.-P. Hauri, and H. Riezman The Emp24 Complex Recruits a Specific Cargo Molecule into Endoplasmic Reticulum-derived Vesicles J. Cell Biol., March 6, 2000; 148(5): 925 - 930. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Ying, T Flatmark, and J Saraste The p58-positive pre-golgi intermediates consist of distinct subpopulations of particles that show differential binding of COPI and COPII coats and contain vacuolar H(+)-ATPase J. Cell Sci., January 10, 2000; 113(20): 3623 - 3638. [Abstract] [PDF]
|
|
|
|
|
|
G Emery, M Rojo, and J Gruenberg Coupled transport of p24 family members J. Cell Sci., January 7, 2000; 113(13): 2507 - 2516. [Abstract] [PDF]
|
|
|
|
|
|
H. Hauri, F Kappeler, H Andersson, and C Appenzeller ERGIC-53 and traffic in the secretory pathway J. Cell Sci., January 2, 2000; 113(4): 587 - 596. [Abstract] [PDF]
|
|
|
|
|
|
R. P. Kuiper, H. R. Waterham, J. Rötter, G. Bouw, and G. J. M. Martens Differential Induction of Two p24delta Putative Cargo Receptors upon Activation of a Prohormoneproducing Cell Mol. Biol. Cell, January 1, 2000; 11(1): 131 - 140. [Abstract] [Full Text]
|
|
|
|
|
|
C. Alvarez, H. Fujita, A. Hubbard, and E. Sztul ER to Golgi Transport: Requirement for p115 at a Pre-Golgi VTC Stage J. Cell Biol., December 13, 1999; 147(6): 1205 - 1222. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. White, L. Johannes, F. Mallard, A. Girod, S. Grill, S. Reinsch, P. Keller, B. Tzschaschel, A. Echard, B. Goud, et al. Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells J. Cell Biol., November 15, 1999; 147(4): 743 - 760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Fullekrug, P Scheiffele, and K Simons VIP36 localisation to the early secretory pathway J. Cell Sci., January 9, 1999; 112(17): 2813 - 2821. [Abstract] [PDF]
|
|
|
|
|
|
T. Zhang and W. Hong Ykt6 Forms a SNARE Complex with Syntaxin 5, GS28, and Bet1 and Participates in a Late Stage in Endoplasmic Reticulum-Golgi Transport J. Biol. Chem., July 13, 2001; 276(29): 27480 - 27487. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lanoix, J. Ouwendijk, A. Stark, E. Szafer, D. Cassel, K. Dejgaard, M. Weiss, and T. Nilsson Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1 J. Cell Biol., December 24, 2001; 155(7): 1199 - 1212. [Abstract] [Full Text] [PDF]
|
|
