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Vol. 10, Issue 6, 2051-2062, June 1999

Direct Visualization of a Protein Nuclear Architecture

Michael J. Hendzel,^* F.-Michel Boisvert, and David P. Bazett-Jones

Department of Cell Biology and Anatomy, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada T2N 4N1

Whether the cell nucleus is organized by an underlying architecture analagous to the cytoskeleton has been a highly contentious issue since the original isolation of a nuclease and salt-resistant nuclear matrix. Despite electron microscopy studies that show that a nuclear architecture can be visualized after fractionation, the necessity to elute chromatin to visualize this structure has hindered general acceptance of a karyoskeleton. Using an analytical electron microscopy method capable of quantitative elemental analysis, electron spectroscopic imaging, we show that the majority of the fine structure within interchromatin regions of the cell nucleus in fixed whole cells is not nucleoprotein. Rather, this fine structure is compositionally similar to known protein-based cellular structures of the cytoplasm. This study is the first demonstration of a protein network in unfractionated and uninfected cells and provides a method for the ultrastructural characterization of the interaction of this protein architecture with chromatin and ribonucleoprotein elements of the cell nucleus.

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^*   Present address: Department of Oncology and Cross Cancer Institute, University of Alberta, 11560 University Avenue, Edmonton, Alberta, Canada T6G 1Z2.
   Corresponding author. E-mail address: bazett{at}acs.ucalgary.ca.

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Molecular Biology of the Cell
Vol. 10, 2051-2062, June 1999
Copyright © 1999 by The American Society for Cell Biology

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