![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 7, 2191-2197, July 1999
Department of Biochemistry, Stanford University School of Medicine,
Stanford, California 94305-5307
Late endosomes and the Golgi complex maintain their cellular
localizations by virtue of interactions with the microtubule-based cytoskeleton. We study the transport of mannose 6-phosphate receptors from late endosomes to the trans-Golgi network in vitro.
We show here that this process is facilitated by microtubules and the microtubule-based motor cytoplasmic dynein; transport is inhibited by
excess recombinant dynamitin or purified microtubule-associated proteins. Mapmodulin, a protein that interacts with the
microtubule-associated proteins MAP2, MAP4, and tau, stimulates the
microtubule- and dynein-dependent localization of Golgi complexes in
semi-intact Chinese hamster ovary cells. The present study shows
that mapmodulin also stimulates the initial rate with which mannose
6-phosphate receptors are transported from late endosomes to the
trans-Golgi network in vitro. These findings represent
the first indication that mapmodulin can stimulate a vesicle transport
process, and they support a model in which the microtubule-based
cytoskeleton enhances the efficiency of vesicle transport between
membrane-bound compartments in mammalian cells.
Present address: Department of Food Engineering and
Biotechnology, The Technion, 32000 Haifa, Israel.
Corresponding author. E-mail address:
pfeffer{at}cmgm.stanford.edu.
This article has been cited by other articles:
|
|
 |
|
  G. L. Hayes, F. C. Brown, A. K. Haas, R. M. Nottingham, F. A. Barr, and S. R. Pfeffer Multiple Rab GTPase Binding Sites in GCC185 Suggest a Model for Vesicle Tethering at the Trans-Golgi Mol. Biol. Cell, January 1, 2009; 20(1): 209 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hehnly, D. Sheff, and M. Stamnes Shiga Toxin Facilitates Its Retrograde Transport by Modifying Microtubule Dynamics Mol. Biol. Cell, October 1, 2006; 17(10): 4379 - 4389. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. G. Ganley and S. R. Pfeffer Cholesterol Accumulation Sequesters Rab9 and Disrupts Late Endosome Function in NPC1-deficient Cells J. Biol. Chem., June 30, 2006; 281(26): 17890 - 17899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. G. Ganley, K. Carroll, L. Bittova, and S. Pfeffer Rab9 GTPase Regulates Late Endosome Size and Requires Effector Interaction for Its Stability Mol. Biol. Cell, December 1, 2004; 15(12): 5420 - 5430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. U. Lauvrak, M. L. Torgersen, and K. Sandvig Efficient endosome-to-Golgi transport of Shiga toxin is dependent on dynamin and clathrin J. Cell Sci., May 1, 2004; 117(11): 2321 - 2331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ghosh-Roy, M. Kulkarni, V. Kumar, S. Shirolikar, and K. Ray Cytoplasmic Dynein-Dynactin Complex Is Required for Spermatid Growth but Not Axoneme Assembly in Drosophila Mol. Biol. Cell, May 1, 2004; 15(5): 2470 - 2483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Opal, J. J. Garcia, A. E. McCall, B. Xu, E. J. Weeber, J. D. Sweatt, H. T. Orr, and H. Y. Zoghbi Generation and Characterization of LANP/pp32 Null Mice Mol. Cell. Biol., April 15, 2004; 24(8): 3140 - 3149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. C. Ikonomov, D. Sbrissa, K. Mlak, R. Deeb, J. Fligger, A. Soans, R. L. Finley Jr., and A. Shisheva Active PIKfyve Associates with and Promotes the Membrane Attachment of the Late Endosome-to-trans-Golgi Network Transport Factor Rab9 Effector p40 J. Biol. Chem., December 19, 2003; 278(51): 50863 - 50871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Opal, J. J. Garcia, F. Propst, A. Matilla, H. T. Orr, and H. Y. Zoghbi Mapmodulin/Leucine-rich Acidic Nuclear Protein Binds the Light Chain of Microtubule-associated Protein 1B and Modulates Neuritogenesis J. Biol. Chem., September 5, 2003; 278(36): 34691 - 34699. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Guillaud, M. Setou, and N. Hirokawa KIF17 Dynamics and Regulation of NR2B Trafficking in Hippocampal Neurons J. Neurosci., January 1, 2003; 23(1): 131 - 140. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Barbero, L. Bittova, and S. R. Pfeffer Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells J. Cell Biol., February 4, 2002; 156(3): 511 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. U. Lauvrak, A. Llorente, T.-G. Iversen, and K. Sandvig Selective regulation of the Rab9-independent transport of ricin to the Golgi apparatus by calcium J. Cell Sci., January 9, 2002; 115(17): 3449 - 3456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Alonso, J. Miskin, B. Hernaez, P. Fernandez-Zapatero, L. Soto, C. Canto, I. Rodriguez-Crespo, L. Dixon, and J. M. Escribano African Swine Fever Virus Protein p54 Interacts with the Microtubular Motor Complex through Direct Binding to Light-Chain Dynein J. Virol., October 15, 2001; 75(20): 9819 - 9827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Walenta, A. J. Didier, X. Liu, and H. Kramer The Golgi-Associated Hook3 Protein Is a Member of a Novel Family of Microtubule-Binding Proteins J. Cell Biol., March 5, 2001; 152(5): 923 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A Habermann, T. Schroer, G Griffiths, and J. Burkhardt Immunolocalization of cytoplasmic dynein and dynactin subunits in cultured macrophages: enrichment on early endocytic organelles J. Cell Sci., January 1, 2001; 114(1): 229 - 240. [Abstract] [PDF]
|
|
|
|
|
|
V. Patki, J. Buxton, A. Chawla, L. Lifshitz, K. Fogarty, W. Carrington, R. Tuft, and S. Corvera Insulin Action on GLUT4 Traffic Visualized in Single 3T3-L1 Adipocytes by Using Ultra-fast Microscopy Mol. Biol. Cell, January 1, 2001; 12(1): 129 - 141. [Abstract] [Full Text]
|
|
|
|
 |
|
 Nadia Khelef, T. T. Soe, O. Quehenberger, N. Beatini, I. Tabas, and F. R. Maxfield Enrichment of Acyl Coenzyme A:Cholesterol O-Acyltransferase Near Trans-Golgi Network and Endocytic Recycling Compartment Arterioscler Thromb Vasc Biol, July 1, 2000; 20(7): 1769 - 1776. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Somsel Rodman and A Wandinger-Ness Rab GTPases coordinate endocytosis J. Cell Sci., January 1, 2000; 113(2): 183 - 192. [Abstract] [PDF]
|
|
|
|
|
|
J. P. Krise, P. M. Sincock, J. G. Orsel, and S. R. Pfeffer Quantitative Analysis of TIP47-Receptor Cytoplasmic Domain Interactions. IMPLICATIONS FOR ENDOSOME-TO-TRANS GOLGI NETWORK TRAFFICKING J. Biol. Chem., August 11, 2000; 275(33): 25188 - 25193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Barbero, L. Bittova, and S. R. Pfeffer Visualization of Rab9-mediated vesicle transport from endosomes to the trans-Golgi in living cells J. Cell Biol., February 4, 2002; 156(3): 511 - 518. [Abstract] [Full Text] [PDF]
|
|
