QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zheng, H. Articles by Raikhel, N. V. Search for Related Content PubMed PubMed Citation Articles by Zheng, H. Articles by Raikhel, N. V.

Vol. 10, Issue 7, 2251-2264, July 1999

The Plant Vesicle-associated SNARE AtVTI1a Likely Mediates Vesicle Transport from the Trans-Golgi Network to the Prevacuolar Compartment

Haiyan Zheng,^* Gabriele Fischer von Mollard,^§ Valentina Kovaleva,^* Tom H. Stevens, and Natasha V. Raikhel^*

 ^*Department of Energy-Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824; and  Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403-1229

Membrane traffic in eukaryotic cells relies on recognition between v-SNAREs on transport vesicles and t-SNAREs on target membranes. Here we report the identification of AtVTI1a and AtVTI1b, two Arabidopsis homologues of the yeast v-SNARE Vti1p, which is required for multiple transport steps in yeast. AtVTI1a and AtVTI1b share 60% amino acid identity with one another and are 32 and 30% identical to the yeast protein, respectively. By suppressing defects found in specific strains of yeast vti1 temperature-sensitive mutants, we show that AtVTI1a can substitute for Vti1p in Golgi-to-prevacuolar compartment (PVC) transport, whereas AtVTI1b substitutes in two alternative pathways: the vacuolar import of alkaline phosphatase and the so-called cytosol-to-vacuole pathway used by aminopeptidase I. Both AtVTI1a and AtVTI1b are expressed in all major organs of Arabidopsis. Using subcellular fractionation and immunoelectron microscopy, we show that AtVTI1a colocalizes with the putative vacuolar cargo receptor AtELP on the trans-Golgi network and the PVC. AtVTI1a also colocalizes with the t-SNARE AtPEP12p to the PVC. In addition, AtVTI1a and AtPEP12p can be coimmunoprecipitated from plant cell extracts. We propose that AtVTI1a functions as a v-SNARE responsible for targeting AtELP-containing vesicles from the trans-Golgi network to the PVC, and that AtVTI1b is involved in a different membrane transport process.

---

   These authors contributed equally to this work.
^§   Present address: Georg-August-University, Biochemie II, Göttingen, Germany 37073.
   Corresponding author. E-mail address: nraikhel{at}pilot.msu.edu.

---

Molecular Biology of the Cell
Vol. 10, 2251-2264, July 1999
Copyright © 1999 by The American Society for Cell Biology

This article has been cited by other articles:

				R. U. Rehman, E. Stigliano, G. W. Lycett, L. Sticher, F. Sbano, M. Faraco, G. Dalessandro, and G.-P. Di Sansebastiano Tomato Rab11a Characterization Evidenced a Difference Between SYP121-Dependent and SYP122-Dependent exocytosis Plant Cell Physiol., May 1, 2008; 49(5): 751 - 766. [Abstract] [Full Text] [PDF]

				E. J. Sohn, M. Rojas-Pierce, S. Pan, C. Carter, A. Serrano-Mislata, F. Madueno, E. Rojo, M. Surpin, and N. V. Raikhel The shoot meristem identity gene TFL1 is involved in flower development and trafficking to the protein storage vacuole PNAS, November 20, 2007; 104(47): 18801 - 18806. [Abstract] [Full Text] [PDF]

				G.-J. Lee, H. Kim, H. Kang, M. Jang, D. W. Lee, S. Lee, and I. Hwang EpsinR2 Interacts with Clathrin, Adaptor Protein-3, AtVTI12, and Phosphatidylinositol-3-Phosphate. Implications for EpsinR2 Function in Protein Trafficking in Plant Cells Plant Physiology, April 1, 2007; 143(4): 1561 - 1575. [Abstract] [Full Text] [PDF]

				M. Sanmartin, A. Ordonez, E. J. Sohn, S. Robert, J. J. Sanchez-Serrano, M. A. Surpin, N. V. Raikhel, and E. Rojo Divergent functions of VTI12 and VTI11 in trafficking to storage and lytic vacuoles in Arabidopsis PNAS, February 27, 2007; 104(9): 3645 - 3650. [Abstract] [Full Text] [PDF]

				J. Song, M. H. Lee, G.-J. Lee, C. M. Yoo, and I. Hwang Arabidopsis EPSIN1 Plays an Important Role in Vacuolar Trafficking of Soluble Cargo Proteins in Plant Cells via Interactions with Clathrin, AP-1, VTI11, and VSR1 PLANT CELL, September 1, 2006; 18(9): 2258 - 2274. [Abstract] [Full Text] [PDF]

				O. Foresti, L. L.P. daSilva, and J. Denecke Overexpression of the Arabidopsis Syntaxin PEP12/SYP21 Inhibits Transport from the Prevacuolar Compartment to the Lytic Vacuole in Vivo PLANT CELL, September 1, 2006; 18(9): 2275 - 2293. [Abstract] [Full Text] [PDF]

				J. B. Heo, H. S. Rho, S. W. Kim, S. M. Hwang, H. J. Kwon, M. Y. Nahm, W. Y. Bang, and J. D. Bahk OsGAP1 Functions as a Positive Regulator of OsRab11-mediated TGN to PM or Vacuole Trafficking Plant Cell Physiol., December 1, 2005; 46(12): 2005 - 2018. [Abstract] [Full Text] [PDF]

				M. Palmieri and J. Z. Kiss Disruption of the F-actin cytoskeleton limits statolith movement in Arabidopsis hypocotyls J. Exp. Bot., September 1, 2005; 56(419): 2539 - 2550. [Abstract] [Full Text] [PDF]

				I. Ohtomo, H. Ueda, T. Shimada, C. Nishiyama, Y. Komoto, I. Hara-Nishimura, and T. Takahashi Identification of an Allele of VAM3/SYP22 that Confers a Semi-dwarf Phenotype in Arabidopsis thaliana Plant Cell Physiol., August 1, 2005; 46(8): 1358 - 1365. [Abstract] [Full Text] [PDF]

				H. Kim, M. Park, S. J. Kim, and I. Hwang Actin Filaments Play a Critical Role in Vacuolar Trafficking at the Golgi Complex in Plant Cells PLANT CELL, March 1, 2005; 17(3): 888 - 902. [Abstract] [Full Text] [PDF]

				E. Lobstein, A. Guyon, M. Ferault, D. Twell, G. Pelletier, and S. Bonhomme The Putative Arabidopsis Homolog of Yeast Vps52p Is Required for Pollen Tube Elongation, Localizes to Golgi, and Might Be Involved in Vesicle Trafficking Plant Physiology, July 1, 2004; 135(3): 1480 - 1490. [Abstract] [Full Text] [PDF]

				M. L. Preuss, J. Serna, T. G. Falbel, S. Y. Bednarek, and E. Nielsen The Arabidopsis Rab GTPase RabA4b Localizes to the Tips of Growing Root Hair Cells PLANT CELL, June 1, 2004; 16(6): 1589 - 1603. [Abstract] [Full Text] [PDF]

				M. Surpin, H. Zheng, M. T. Morita, C. Saito, E. Avila, J. J. Blakeslee, A. Bandyopadhyay, V. Kovaleva, D. Carter, A. Murphy, et al. The VTI Family of SNARE Proteins Is Necessary for Plant Viability and Mediates Different Protein Transport Pathways PLANT CELL, December 1, 2003; 15(12): 2885 - 2899. [Abstract] [Full Text] [PDF]

				D. Yano, M. Sato, C. Saito, M. H. Sato, M. T. Morita, and M. Tasaka A SNARE complex containing SGR3/AtVAM3 and ZIG/VTI11 in gravity-sensing cells is important for Arabidopsis shoot gravitropism PNAS, July 8, 2003; 100(14): 8589 - 8594. [Abstract] [Full Text] [PDF]

				M. H. Lee, M. K. Min, Y. J. Lee, J. B. Jin, D. H. Shin, D. H. Kim, K.-H. Lee, and I. Hwang ADP-Ribosylation Factor 1 of Arabidopsis Plays a Critical Role in Intracellular Trafficking and Maintenance of Endoplasmic Reticulum Morphology in Arabidopsis Plant Physiology, August 1, 2002; 129(4): 1507 - 1520. [Abstract] [Full Text] [PDF]

				H. Zheng, S. Y. Bednarek, A. A. Sanderfoot, J. Alonso, J. R. Ecker, and N. V. Raikhel NPSN11 Is a Cell Plate-Associated SNARE Protein That Interacts with the Syntaxin KNOLLE Plant Physiology, June 1, 2002; 129(2): 530 - 539. [Abstract] [Full Text] [PDF]

				E. Rojo, V. K. Sharma, V. Kovaleva, N. V. Raikhel, and J. C. Fletcher CLV3 Is Localized to the Extracellular Space, Where It Activates the Arabidopsis CLAVATA Stem Cell Signaling Pathway PLANT CELL, May 1, 2002; 14(5): 969 - 977. [Abstract] [Full Text] [PDF]

				T. Inaba, Y. Nagano, T. Nagasaki, and Y. Sasaki Distinct Localization of Two Closely Related Ypt3/Rab11 Proteins on the Trafficking Pathway in Higher Plants J. Biol. Chem., March 8, 2002; 277(11): 9183 - 9188. [Abstract] [Full Text] [PDF]

				F. Mallard, B. L. Tang, T. Galli, D. Tenza, A. Saint-Pol, X. Yue, C. Antony, W. Hong, B. Goud, and L. Johannes Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform J. Cell Biol., February 18, 2002; 156(4): 653 - 664. [Abstract] [Full Text] [PDF]

				D. Geelen, B. Leyman, H. Batoko, G.-P. Di Sansebastiano, I. Moore, and M. R. Blatt The Abscisic Acid-Related SNARE Homolog NtSyr1 Contributes to Secretion and Growth: Evidence from Competition with Its Cytosolic Domain PLANT CELL, February 1, 2002; 14(2): 387 - 406. [Abstract] [Full Text] [PDF]

				A. A. Sanderfoot, V. Kovaleva, D. C. Bassham, and N. V. Raikhel Interactions between Syntaxins Identify at Least Five SNARE Complexes within the Golgi/Prevacuolar System of the Arabidopsis Cell Mol. Biol. Cell, December 1, 2001; 12(12): 3733 - 3743. [Abstract] [Full Text] [PDF]

				J. B. Jin, Y. A Kim, S. J. Kim, S. H. Lee, D. H. Kim, G.-W. Cheong, and I. Hwang A New Dynamin-Like Protein, ADL6, Is Involved in Trafficking from the trans-Golgi Network to the Central Vacuole in Arabidopsis PLANT CELL, July 1, 2001; 13(7): 1511 - 1526. [Abstract] [Full Text] [PDF]

				D. H. Kim, Y.-J. Eu, C. M. Yoo, Y.-W. Kim, K. T. Pih, J. B. Jin, S. J. Kim, H. Stenmark, and I. Hwang Trafficking of Phosphatidylinositol 3-Phosphate from the trans-Golgi Network to the Lumen of the Central Vacuole in Plant Cells PLANT CELL, February 1, 2001; 13(2): 287 - 301. [Abstract] [Full Text]

				Y. Lin, D. F. Seals, S. K. Randall, and Z. Yang Dynamic Localization of Rop GTPases to the Tonoplast during Vacuole Development Plant Physiology, January 1, 2001; 125(1): 241 - 251. [Abstract] [Full Text]

				A. A. Sanderfoot, F. F. Assaad, and N. V. Raikhel The Arabidopsis Genome. An Abundance of Soluble N-Ethylmaleimide-Sensitive Factor Adaptor Protein Receptors Plant Physiology, December 1, 2000; 124(4): 1558 - 1569. [Abstract] [Full Text]

				D. C. Bassham, A. A. Sanderfoot, V. Kovaleva, H. Zheng, and N. V. Raikhel AtVPS45 Complex Formation at the trans-Golgi Network Mol. Biol. Cell, July 1, 2000; 11(7): 2251 - 2265. [Abstract] [Full Text]

				S. U. Ahmed, E. Rojo, V. Kovaleva, S. Venkataraman, J. E. Dombrowski, K. Matsuoka, and N. V. Raikhel The Plant Vacuolar Sorting Receptor AtELP Is Involved in Transport of NH2-terminal Propeptide-containing Vacuolar Proteins in Arabidopsis thaliana J. Cell Biol., June 26, 2000; 149(7): 1335 - 1344. [Abstract] [Full Text] [PDF]

				D. C. Bassham and N. V. Raikhel Plant Cells Are Not Just Green Yeast Plant Physiology, April 1, 2000; 122(4): 999 - 1002. [Full Text]

				M. Gasparian, M. Pusterla, B. Baldan, P. M. Downey, O. Rossetto, P. P. de Laureto, F. Filippini, M. Terzi, and F. L. Schiavo Identification and Characterization of an 18-Kilodalton, VAMP-Like Protein in Suspension-Cultured Carrot Cells Plant Physiology, January 1, 2000; 122(1): 25 - 34. [Abstract] [Full Text] [PDF]

				A. A. Sanderfoot, V. Kovaleva, H. Zheng, and N. V. Raikhel The t-SNARE AtVAM3p Resides on the Prevacuolar Compartment in Arabidopsis Root Cells Plant Physiology, November 1, 1999; 121(3): 929 - 938. [Abstract] [Full Text]

				F. Mallard, B. L. Tang, T. Galli, D. Tenza, A. Saint-Pol, X. Yue, C. Antony, W. Hong, B. Goud, and L. Johannes Early/recycling endosomes-to-TGN transport involves two SNARE complexes and a Rab6 isoform J. Cell Biol., February 18, 2002; 156(4): 653 - 664. [Abstract] [Full Text] [PDF]

				T. Kato, M. T. Morita, H. Fukaki, Y. Yamauchi, M. Uehara, M. Niihama, and M. Tasaka SGR2, a Phospholipase-Like Protein, and ZIG/SGR4, a SNARE, Are Involved in the Shoot Gravitropism of Arabidopsis PLANT CELL, January 1, 2002; 14(1): 33 - 46. [Abstract] [Full Text] [PDF]

				M. T. Morita, T. Kato, K. Nagafusa, C. Saito, T. Ueda, A. Nakano, and M. Tasaka Involvement of the Vacuoles of the Endodermis in the Early Process of Shoot Gravitropism in Arabidopsis PLANT CELL, January 1, 2002; 14(1): 47 - 56. [Abstract] [Full Text] [PDF]