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Vol. 10, Issue 7, 2297-2307, July 1999
Department of Cell Biology, John Innes Centre, Norwich NR4 7UH,
United Kingdom
Coiled bodies are nuclear organelles that contain components of at
least three RNA-processing pathways: pre-mRNA splicing, histone mRNA
3'- maturation, and pre-rRNA processing. Their function remains
unknown. However, it has been speculated that coiled bodies may be
sites of splicing factor assembly and/or recycling, play a role in
histone mRNA 3'-processing, or act as nuclear transport or sorting
structures. To study the dynamics of coiled bodies in living cells, we
have stably expressed a U2B"-green fluorescent protein fusion in
tobacco BY-2 cells and in Arabidopsis plants. Time-lapse
confocal microscopy has shown that coiled bodies are mobile organelles
in plant cells. We have observed movements of coiled bodies in the
nucleolus, in the nucleoplasm, and from the periphery of the nucleus
into the nucleolus, which suggests a transport function for coiled
bodies. Furthermore, we have observed coalescence of coiled bodies,
which suggests a mechanism for the decrease in coiled body number
during the cell cycle. Deletion analysis of the U2B"
gene construct has shown that the first RNP-80 motif is sufficient for
localization to the coiled body.
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