![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 7, 2361-2375, July 1999
*Division of Experimental Medicine, Beth Israel Deaconess Medical
Center, Harvard Institutes of Medicine, Boston, Massachusetts 02115;
The cytoskeleton plays an important role in neuronal morphogenesis.
We have identified and characterized a novel actin-binding protein,
termed Mayven, predominantly expressed in brain. Mayven contains a BTB
(broad complex, tramtrack, bric-a-brac)/POZ (poxvirus, zinc finger)
domain-like structure in the predicted N terminus and "kelch
repeats" in the predicted C-terminal domain. Mayven shares 63%
identity (77% similarity) with the Drosophila ring canal ("kelch") protein. Somatic cell-hybrid analysis indicated that the human Mayven gene is located on chromosome 4q21.2, whereas the
murine homolog gene is located on chromosome 8. The BTB/POZ domain of
Mayven can self-dimerize in vitro, which might be important for its
interaction with other BTB/POZ-containing proteins. Confocal microscopic studies of endogenous Mayven protein revealed a highly dynamic localization pattern of the protein. In U373-MG
astrocytoma/glioblastoma cells, Mayven colocalized with actin filaments
in stress fibers and in patchy cortical actin-rich regions of the cell
margins. In primary rat hippocampal neurons, Mayven is highly expressed in the cell body and in neurite processes. Binding assays and far
Western blotting analysis demonstrated association of Mayven with
actin. This association is mediated through the "kelch repeats" within the C terminus of Mayven. Depolarization of primary hippocampal neurons with KCl enhanced the association of Mayven with actin. This
increased association resulted in dynamic changes in Mayven distribution from uniform to punctate localization along neuronal processes. These results suggest that Mayven functions as an
actin-binding protein that may be translocated along axonal processes
and might be involved in the dynamic organization of the actin
cytoskeleton in brain cells.
BioMedical Imaging Laboratory, Harvard School of Public
Health, Boston, Massachusetts 02115; and Section of
Medical Genetics and Molecular Medicine, Children's Mercy Hospital,
University of Missouri Kansas City School of Medicine, Kansas
City, Missouri 64108
Corresponding author.
This article has been cited by other articles:
|
|
 |
|
  S. Jiang, S. Seng, H. K. Avraham, Y. Fu, and S. Avraham Process Elongation of Oligodendrocytes Is Promoted by the Kelch-related Protein MRP2/KLHL1 J. Biol. Chem., April 20, 2007; 282(16): 12319 - 12329. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Seng, H. K. Avraham, S. Jiang, S. Venkatesh, and S. Avraham KLHL1/MRP2 Mediates Neurite Outgrowth in a Glycogen Synthase Kinase 3{beta}-Dependent Manner Mol. Cell. Biol., November 15, 2006; 26(22): 8371 - 8384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-A. Kim, S. Jiang, S. Seng, K. Cha, H. K. Avraham, and S. Avraham The BTB domain of the nuclear matrix protein NRP/B is required for neurite outgrowth J. Cell Sci., December 1, 2005; 118(23): 5537 - 5548. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Demir, P Bomont, S Erdem, L Cavalier, M Demirci, G Kose, S Muftuoglu, A N Cakar, E Tan, S Aysun, et al. Giant axonal neuropathy: clinical and genetic study in six cases J. Neurol. Neurosurg. Psychiatry, June 1, 2005; 76(6): 825 - 832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Gingerich, J. M. Gagne, D. W. Salter, H. Hellmann, M. Estelle, L. Ma, and R. D. Vierstra Cullins 3a and 3b Assemble with Members of the Broad Complex/Tramtrack/Bric-a-Brac (BTB) Protein Family to Form Essential Ubiquitin-Protein Ligases (E3s) in Arabidopsis J. Biol. Chem., May 13, 2005; 280(19): 18810 - 18821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Heiska and O. Carpen Src Phosphorylates Ezrin at Tyrosine 477 and Induces a Phosphospecific Association between Ezrin and a Kelch-Repeat Protein Family Member J. Biol. Chem., March 18, 2005; 280(11): 10244 - 10252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li, D. Zhang, M. Hannink, and L. J. Beamer Crystal Structure of the Kelch Domain of Human Keap1 J. Biol. Chem., December 24, 2004; 279(52): 54750 - 54758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ishida, X. Zhang, K. Erickson, and P. Ray Botulinum Toxin Type A Targets RhoB to Inhibit Lysophosphatidic Acid-Stimulated Actin Reorganization and Acetylcholine Release in Nerve Growth Factor-Treated PC12 Cells J. Pharmacol. Exp. Ther., September 1, 2004; 310(3): 881 - 889. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Yan, L. Ma, K. H. Burns, and M. M. Matzuk Haploinsufficiency of kelch-like protein homolog 10 causes infertility in male mice PNAS, May 18, 2004; 101(20): 7793 - 7798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hara, H. Ishida, R. Raziuddin, S. Dorkhom, K. Kamijo, and T. Miki Novel Kelch-like Protein, KLEIP, Is Involved in Actin Assembly at Cell-Cell Contact Sites of Madin-Darby Canine Kidney Cells Mol. Biol. Cell, March 1, 2004; 15(3): 1172 - 1184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-I. Kang, A. Kobayashi, N. Wakabayashi, S.-G. Kim, and M. Yamamoto Scaffolding of Keap1 to the actin cytoskeleton controls the function of Nrf2 as key regulator of cytoprotective phase 2 genes PNAS, February 17, 2004; 101(7): 2046 - 2051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sasagawa, Y. Matsudo, M. Kang, L. Fujimura, Y. Iitsuka, S. Okada, T. Ochiai, T. Tokuhisa, and M. Hatano Identification of Nd1, a Novel Murine Kelch Family Protein, Involved in Stabilization of Actin Filaments J. Biol. Chem., November 8, 2002; 277(46): 44140 - 44146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Zipper and R. T. Mulcahy The Keap1 BTB/POZ Dimerization Function Is Required to Sequester Nrf2 in Cytoplasm J. Biol. Chem., September 20, 2002; 277(39): 36544 - 36552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, R. Derin, R. S. Petralia, and a. M. Li Actinfilin, a Brain-specific Actin-binding Protein in Postsynaptic Density J. Biol. Chem., August 16, 2002; 277(34): 30495 - 30501. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Kelso, A. M. Hudson, and L. Cooley Drosophila Kelch regulates actin organization via Src64-dependent tyrosine phosphorylation J. Cell Biol., February 18, 2002; 156(4): 703 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Fujita, Y. Furukawa, T. Tsunoda, T. Tanaka, M. Ogawa, and Y. Nakamura Up-Regulation of the Ectodermal-Neural Cortex 1 (ENC1) Gene, a Downstream Target of the {beta}-Catenin/T-Cell Factor Complex, in Colorectal Carcinomas Cancer Res., November 1, 2001; 61(21): 7722 - 7726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Zhao, F. Gregoire, and H. Sook Sul Transient Induction of ENC-1, a Kelch-related Actin-binding Protein, Is Required for Adipocyte Differentiation J. Biol. Chem., May 26, 2000; 275(22): 16845 - 16850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Kelso, A. M. Hudson, and L. Cooley Drosophila Kelch regulates actin organization via Src64-dependent tyrosine phosphorylation J. Cell Biol., February 18, 2002; 156(4): 703 - 713. [Abstract] [Full Text] [PDF]
|
|
