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Vol. 10, Issue 7, 2377-2391, July 1999
Department of Molecular, Cellular, and Developmental Biology,
University of Colorado-Boulder, Boulder Colorado 80309-0347
We have identified a mutant allele of the DAM1 gene
in a screen for mutations that are lethal in combination with the
mps1-1 mutation. MPS1 encodes an
essential protein kinase that is required for duplication of the
spindle pole body and for the spindle assembly checkpoint. Mutations in
six different genes were found to be lethal in combination with
mps1-1, of which only DAM1 was novel. The
remaining genes encode a checkpoint protein, Bub1p, and four chaperone
proteins, Sti1p, Hsc82p, Cdc37p, and Ydj1p. DAM1 is an
essential gene that encodes a protein recently described as a member of
a microtubule binding complex. We report here that cells
harboring the dam1-1 mutation fail to maintain spindle
integrity during anaphase at the restrictive temperature. Consistent
with this phenotype, DAM1 displays genetic interactions
with STU1, CIN8, and KAR3, genes encoding
proteins involved in spindle function. We have observed that a
Dam1p-Myc fusion protein expressed at endogenous levels and localized
by immunofluorescence microscopy, appears to be evenly distributed
along short mitotic spindles but is found at the spindle poles at later
times in mitosis.
Corresponding author. E-mail address:
Mark.Winey{at}Colorado.edu.
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