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Vol. 10, Issue 7, 2393-2406, July 1999
*Service de Biochimie et de Génétique
Moléculaire, Commissariat à l'Energie
Atomique/Saclay, F-91191 Gif-sur-Yvette, France;
The MPS2 (monopolar spindle two) gene is one of several
genes required for the proper execution of spindle pole body (SPB) duplication in the budding yeast Saccharomyces
cerevisiae (Winey et al., 1991). We report here
that the MPS2 gene encodes an essential 44-kDa protein
with two putative coiled-coil regions and a hydrophobic sequence.
Although MPS2 is required for normal mitotic growth, some null strains can survive; these survivors exhibit slow growth and
abnormal ploidy. The MPS2 protein was tagged with nine
copies of the myc epitope, and biochemical fractionation experiments show that it is an integral membrane protein. Visualization of a green
fluorescent protein (GFP) Mps2p fusion protein in living cells
and indirect immunofluorescence microscopy of 9xmyc-Mps2p revealed a
perinuclear localization with one or two brighter foci of staining
corresponding to the SPB. Additionally, immunoelectron microscopy shows
that GFP-Mps2p localizes to the SPB. Our analysis suggests that Mps2p
is required as a component of the SPB for insertion of the nascent SPB
into the nuclear envelope.
Department of Molecular, Cellular, and Developmental
Biology, University of Colorado-Boulder, Boulder, Colorado 80309-0347;
and §Department of Genetics, University of Washington,
Seattle, Washington 98195
These authors contributed equally to this work.
Corresponding author. E-mail address:
Mark.Winey{at}Colorado.edu.
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