![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 8, 2507-2518, August 1999
*Department of Cell Biology and Anatomy and
The focal adhesion kinase (FAK) is discretely localized to focal
adhesions via its C-terminal focal adhesion-targeting (FAT) sequence. FAK is regulated by integrin-dependent cell adhesion and can regulate tyrosine phosphorylation of downstream substrates, like paxillin. By the use of a mutational strategy, the regions of FAK
that are required for cell adhesion-dependent regulation and for
inducing tyrosine phosphorylation of paxillin were determined. The
results show that the FAT sequence was the single region of FAK that
was required for each function. Furthermore, the FAT sequence of FAK
was replaced with a focal adhesion-targeting sequence from vinculin,
and the resulting chimera exhibited cell adhesion-dependent tyrosine
phosphorylation and could induce paxillin phosphorylation like
wild-type FAK. These results suggest that subcellular localization is
the major determinant of FAK function.
Lineberger Comprehensive Cancer Center, University of
North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599
Corresponding author. E-mail address:
crispy4{at}med.unc.edu.
This article has been cited by other articles:
|
|
 |
|
  P. Bryant, Q. Zheng, and K. Pumiglia Focal Adhesion Kinase Controls Cellular Levels of p27/Kip1 and p21/Cip1 through Skp2-Dependent and -Independent Mechanisms. Mol. Cell. Biol., June 1, 2006; 26(11): 4201 - 4213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Kragtorp and J. R. Miller Regulation of somitogenesis by Ena/VASP proteins and FAK during Xenopus development Development, February 15, 2006; 133(4): 685 - 695. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Moeller, Y. Shi, L. F. Reichardt, and I. M. Ethell EphB Receptors Regulate Dendritic Spine Morphogenesis through the Recruitment/Phosphorylation of Focal Adhesion Kinase and RhoA Activation J. Biol. Chem., January 20, 2006; 281(3): 1587 - 1598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. V. Usatyuk and V. Natarajan Regulation of reactive oxygen species-induced endothelial cell-cell and cell-matrix contacts by focal adhesion kinase and adherens junction proteins Am J Physiol Lung Cell Mol Physiol, December 1, 2005; 289(6): L999 - L1010. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Golubovskaya, R. Finch, and W. G. Cance Direct Interaction of the N-terminal Domain of Focal Adhesion Kinase with the N-terminal Transactivation Domain of p53 J. Biol. Chem., July 1, 2005; 280(26): 25008 - 25021. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Cohen and J.-L. Guan Residues within the First Subdomain of the FERM-like Domain in Focal Adhesion Kinase Are Important in Its Regulation J. Biol. Chem., March 4, 2005; 280(9): 8197 - 8207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Ding, J. R. Grammer, M. A. Nelson, J.-L. Guan, J. E. Stewart Jr., and C. L. Gladson p27Kip1 and Cyclin D1 Are Necessary for Focal Adhesion Kinase Regulation of Cell Cycle Progression in Glioblastoma Cells Propagated in Vitro and in Vivo in the Scid Mouse Brain J. Biol. Chem., February 25, 2005; 280(8): 6802 - 6815. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Giannone, P. Ronde, M. Gaire, J. Beaudouin, J. Haiech, J. Ellenberg, and K. Takeda Calcium Rises Locally Trigger Focal Adhesion Disassembly and Enhance Residency of Focal Adhesion Kinase at Focal Adhesions J. Biol. Chem., July 2, 2004; 279(27): 28715 - 28723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Dunty, V. Gabarra-Niecko, M. L. King, D. F. J. Ceccarelli, M. J. Eck, and M. D. Schaller FERM Domain Interaction Promotes FAK Signaling Mol. Cell. Biol., June 15, 2004; 24(12): 5353 - 5368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Gao, K. C. Prutzman, M. L. King, D. M. Scheswohl, E. F. DeRose, R. E. London, M. D. Schaller, and S. L. Campbell NMR Solution Structure of the Focal Adhesion Targeting Domain of Focal Adhesion Kinase in Complex with a Paxillin LD Peptide: EVIDENCE FOR A TWO-SITE BINDING MODEL J. Biol. Chem., February 27, 2004; 279(9): 8441 - 8451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Cooper, T.-L. Shen, and J.-L. Guan Regulation of Focal Adhesion Kinase by Its Amino-Terminal Domain through an Autoinhibitory Interaction Mol. Cell. Biol., November 15, 2003; 23(22): 8030 - 8041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.-Z. Katz, L. Romer, S. Miyamoto, T. Volberg, K. Matsumoto, E. Cukierman, B. Geiger, and K. M. Yamada Targeting Membrane-localized Focal Adhesion Kinase to Focal Adhesions: ROLES OF TYROSINE PHOSPHORYLATION AND SRC FAMILY KINASES J. Biol. Chem., August 1, 2003; 278(31): 29115 - 29120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Hunger-Glaser, E. P. Salazar, J. Sinnett-Smith, and E. Rozengurt Bombesin, Lysophosphatidic Acid, and Epidermal Growth Factor Rapidly Stimulate Focal Adhesion Kinase Phosphorylation at Ser-910: REQUIREMENT FOR ERK ACTIVATION J. Biol. Chem., June 13, 2003; 278(25): 22631 - 22643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Kwong, M. A. Wozniak, A. S. Collins, S. D. Wilson, and P. J. Keely R-Ras Promotes Focal Adhesion Formation through Focal Adhesion Kinase and p130Cas by a Novel Mechanism That Differs from Integrins Mol. Cell. Biol., February 1, 2003; 23(3): 933 - 949. [Abstract] [Full Text]
|
|
|
|
|
|
M. Toutant, A. Costa, J.-M. Studler, G. Kadare, M. Carnaud, and J.-A. Girault Alternative Splicing Controls the Mechanisms of FAK Autophosphorylation Mol. Cell. Biol., November 15, 2002; 22(22): 7731 - 7743. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Dunty and M. D. Schaller The N Termini of Focal Adhesion Kinase Family Members Regulate Substrate Phosphorylation, Localization, and Cell Morphology J. Biol. Chem., November 15, 2002; 277(47): 45644 - 45654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Martin and P. Leder Human MCF10A Mammary Epithelial Cells Undergo Apoptosis following Actin Depolymerization That Is Independent of Attachment and Rescued by Bcl-2 Mol. Cell. Biol., October 1, 2001; 21(19): 6529 - 6536. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Kovacic-Milivojevic, F. Roediger, E. A.C. Almeida, C. H. Damsky, D. G. Gardner, and D. Ilic Focal Adhesion Kinase and p130Cas Mediate Both Sarcomeric Organization and Activation of Genes Associated with Cardiac Myocyte Hypertrophy Mol. Biol. Cell, August 1, 2001; 12(8): 2290 - 2307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Jones, J. Machado Jr., and A. Merlo Loss of Focal Adhesion Kinase (FAK) Inhibits Epidermal Growth Factor Receptor-dependent Migration and Induces Aggregation of NH2-Terminal FAK in the Nuclei of Apoptotic Glioblastoma Cells Cancer Res., July 1, 2001; 61(13): 4978 - 4981. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. D. WILSON Polycystin: New Aspects of Structure, Function, and Regulation J. Am. Soc. Nephrol., April 1, 2001; 12(4): 834 - 845. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Reddy, C. A. Wass, K. S. Kim, D. D. Schlaepfer, and N. V. Prasadarao Involvement of Focal Adhesion Kinase in Escherichia coli Invasion of Human Brain Microvascular Endothelial Cells Infect. Immun., November 1, 2000; 68(11): 6423 - 6430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Cooley, J. M. Broome, C. Ohngemach, L. H. Romer, and M. D. Schaller Paxillin Binding Is Not the Sole Determinant of Focal Adhesion Localization or Dominant-Negative Activity of Focal Adhesion Kinase/Focal Adhesion Kinase-related Nonkinase Mol. Biol. Cell, September 1, 2000; 11(9): 3247 - 3263. [Abstract] [Full Text]
|
|
|
|
|
|
K. Wennerberg, A. Armulik, T. Sakai, M. Karlsson, R. Fässler, E. M. Schaefer, D. F. Mosher, and S. Johansson The Cytoplasmic Tyrosines of Integrin Subunit beta 1 Are Involved in Focal Adhesion Kinase Activation Mol. Cell. Biol., August 1, 2000; 20(15): 5758 - 5765. [Abstract] [Full Text]
|
|
|
|
|
|
J. W. Thomas, M. A. Cooley, J. M. Broome, R. Salgia, J. D. Griffin, C. R. Lombardo, and M. D. Schaller The Role of Focal Adhesion Kinase Binding in the Regulation of Tyrosine Phosphorylation of Paxillin J. Biol. Chem., December 17, 1999; 274(51): 36684 - 36692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Vial, H. Okazaki, and R. P. Siraganian The NH2-terminal Region of Focal Adhesion Kinase Reconstitutes High Affinity IgE Receptor-induced Secretion in Mast Cells J. Biol. Chem., September 1, 2000; 275(36): 28269 - 28275. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. T. Arold, T. S. Ulmer, T. D. Mulhern, J. M. Werner, J. E. Ladbury, I. D. Campbell, and M. E. M. Noble The Role of the Src Homology 3-Src Homology 2 Interface in the Regulation of Src Kinases J. Biol. Chem., May 11, 2001; 276(20): 17199 - 17205. [Abstract] [Full Text] [PDF]
|
|
