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Vol. 10, Issue 8, 2547-2557, August 1999

tRNAs and Proteins Are Imported into Mitochondria of Trypanosoma brucei by Two Distinct Mechanisms

Christoph E. Nabholz, Elke K. Horn, and André Schneider*

University of Fribourg, Institute of Zoology, Pérolles, CH-1700 Fribourg, Switzerland

Import of tRNA into the mitochondrial matrix of Trypanosoma brucei was reconstituted in vitro. Efficient import required the hydrolysis of externally added ATP and was shown to be a carrier-mediated process depending on proteinaceous receptors on the surface of mitochondria. A partly synthetic tRNATyr as well as a physiological tRNALys were imported along the same pathway. Contrary to import of all matrix-localized proteins, tRNA import does not require a membrane potential. Furthermore, addition of an excess of import-competent tRNA had no effect on import of a mitochondrial matrix protein. In summary, these results show that tRNAs and proteins in T. brucei are imported by fundamentally different mechanisms.

*   Corresponding author. E-mail address: andre.schneider{at}unifr.ch.

Molecular Biology of the Cell
Vol. 10, 2547-2557, August 1999
Copyright © 1999 by The American Society for Cell Biology


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