![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 8, 2687-2702, August 1999
and
*Department of Pharmacology, University of Colorado Health Science
Center, Denver, Colorado 80111; and The clathrin assembly lymphoid myeloid leukemia
(CALM) gene encodes a putative homologue of the clathrin
assembly synaptic protein AP180. Hence the biochemical
properties, the subcellular localization, and the role in endocytosis
of a CALM protein were studied. In vitro binding and
coimmunoprecipitation demonstrated that the clathrin heavy chain is the
major binding partner of CALM. The bulk of cellular CALM was associated
with the membrane fractions of the cell and localized to
clathrin-coated areas of the plasma membrane. In the membrane fraction,
CALM was present at near stoichiometric amounts relative to clathrin.
To perform structure-function analysis of CALM, we engineered chimeric
fusion proteins of CALM and its fragments with the green fluorescent protein (GFP). GFP-CALM was targeted to the plasma membrane-coated pits and also found colocalized with clathrin in the Golgi area. High
levels of expression of GFP-CALM or its fragments with
clathrin-binding activity inhibited the endocytosis of transferrin and
epidermal growth factor receptors and altered the steady-state
distribution of the mannose-6-phosphate receptor in the cell. In
addition, GFP-CALM overexpression caused the loss of clathrin
accumulation in the trans-Golgi network area, whereas the localization
of the clathrin adaptor protein complex 1 in the trans-Golgi network remained unaffected. The ability of the GFP-tagged fragments of CALM to
affect clathrin-mediated processes correlated with the targeting of the
fragments to clathrin-coated areas and their clathrin-binding
capacities. Clathrin-CALM interaction seems to be regulated by
multiple contact interfaces. The C-terminal part of CALM binds
clathrin heavy chain, although the full-length protein exhibited
maximal ability for interaction. Altogether, the data suggest that CALM
is an important component of coated pit internalization machinery,
possibly involved in the regulation of clathrin recruitment to the
membrane and/or the formation of the coated pit.
Institute of Human
Genetics, 37073 Goettingen, Germany
Corresponding author. E-mail address:
alexander.sorkin{at}uchsc.edu.
This article has been cited by other articles:
|
|
 |
|
  Y.-H. Lin, P. M. Kakadia, Y. Chen, Y.-Q. Li, A. J. Deshpande, C. Buske, K.-L. Zhang, Y. Zhang, G.-L. Xu, and S. K. Bohlander Global reduction of the epigenetic H3K79 methylation mark and increased chromosomal instability in CALM-AF10-positive leukemias Blood, July 16, 2009; 114(3): 651 - 658. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Bushlin, R. S. Petralia, F. Wu, A. Harel, M. R. Mughal, M. P. Mattson, and P. J. Yao Clathrin Assembly Protein AP180 and CALM Differentially Control Axogenesis and Dendrite Outgrowth in Embryonic Hippocampal Neurons J. Neurosci., October 8, 2008; 28(41): 10257 - 10271. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Maldonado-Baez, M. R. Dores, E. M. Perkins, T. G. Drivas, L. Hicke, and B. Wendland Interaction between Epsin/Yap180 Adaptors and the Scaffolds Ede1/Pan1 Is Required for Endocytosis Mol. Biol. Cell, July 1, 2008; 19(7): 2936 - 2948. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Olesen, M. G. J. Ford, E. M. Schmid, Y. Vallis, M. M. Babu, P. H. Li, I. G. Mills, H. T. McMahon, and G. J. K. Praefcke Solitary and Repetitive Binding Motifs for the AP2 Complex {alpha}-Appendage in Amphiphysin and Other Accessory Proteins J. Biol. Chem., February 22, 2008; 283(8): 5099 - 5109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Navarro-Garcia, A. Canizalez-Roman, J. E. Vidal, and Ma. I. Salazar Intoxication of epithelial cells by plasmid-encoded toxin requires clathrin-mediated endocytosis Microbiology, September 1, 2007; 153(9): 2828 - 2838. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Caudell, Z. Zhang, Y. J. Chung, and P. D. Aplan Expression of a CALM-AF10 Fusion Gene Leads to Hoxa Cluster Overexpression and Acute Leukemia in Transgenic Mice Cancer Res., September 1, 2007; 67(17): 8022 - 8031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Henderson and S. D. Conner A Novel AAK1 Splice Variant Functions at Multiple Steps of the Endocytic Pathway Mol. Biol. Cell, July 1, 2007; 18(7): 2698 - 2706. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Zhang, Y.-I. Yim, S. Scarselletta, M. Norton, E. Eisenberg, and L. E. Greene Clathrin Adaptor GGA1 Polymerizes Clathrin into Tubules J. Biol. Chem., May 4, 2007; 282(18): 13282 - 13289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Stavrou and T. J. O'Halloran The Monomeric Clathrin Assembly Protein, AP180, Regulates Contractile Vacuole Size in Dictyostelium discoideum Mol. Biol. Cell, December 1, 2006; 17(12): 5381 - 5389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Dho, J. Trejo, D. P. Siderovski, and C. J. McGlade Dynamic Regulation of Mammalian Numb by G Protein-coupled Receptors and Protein Kinase C Activation: Structural Determinants of Numb Association with the Cortical Membrane Mol. Biol. Cell, September 1, 2006; 17(9): 4142 - 4155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Natsume, K. Tanabe, S. Kon, N. Yoshida, T. Watanabe, T. Torii, and M. Satake SMAP2, a Novel ARF GTPase-activating Protein, Interacts with Clathrin and Clathrin Assembly Protein and Functions on the AP-1-positive Early Endosome/Trans-Golgi Network Mol. Biol. Cell, June 1, 2006; 17(6): 2592 - 2603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanzaki, M. Furukawa, W. Raab, and J. E. Pessin Phosphatidylinositol 4,5-Bisphosphate Regulates Adipocyte Actin Dynamics and GLUT4 Vesicle Recycling J. Biol. Chem., July 16, 2004; 279(29): 30622 - 30633. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Roth Phosphoinositides in Constitutive Membrane Traffic Physiol Rev, July 1, 2004; 84(3): 699 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Huang, A. Khvorova, W. Marshall, and A. Sorkin Analysis of Clathrin-mediated Endocytosis of Epidermal Growth Factor Receptor by RNA Interference J. Biol. Chem., April 16, 2004; 279(16): 16657 - 16661. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Barth and S. E. H. Holstein Identification and functional characterization of Arabidopsis AP180, a binding partner of plant {alpha}C-adaptin J. Cell Sci., April 15, 2004; 117(10): 2051 - 2062. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Legendre-Guillemin, S. Wasiak, N. K. Hussain, A. Angers, and P. S. McPherson ENTH/ANTH proteins and clathrin-mediated membrane budding J. Cell Sci., January 1, 2004; 117(1): 9 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Hinrichsen, J. Harborth, L. Andrees, K. Weber, and E. J. Ungewickell Effect of Clathrin Heavy Chain- and {alpha}-Adaptin-specific Small Inhibitory RNAs on Endocytic Accessory Proteins and Receptor Trafficking in HeLa Cells J. Biol. Chem., November 14, 2003; 278(46): 45160 - 45170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Kusner and C. Carlin Potential role for a novel AP180-related protein during endocytosis in MDCK cells Am J Physiol Cell Physiol, November 1, 2003; 285(5): C995 - C1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Conner and S. L. Schmid Differential requirements for AP-2 in clathrin-mediated endocytosis J. Cell Biol., September 1, 2003; 162(5): 773 - 780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Morgan, K. Prasad, S. Jin, G. J. Augustine, and E. M. Lafer Eps15 Homology Domain-NPF Motif Interactions Regulate Clathrin Coat Assembly during Synaptic Vesicle Recycling J. Biol. Chem., August 29, 2003; 278(35): 33583 - 33592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Asnafi, I. Radford-Weiss, N. Dastugue, C. Bayle, D. Leboeuf, C. Charrin, R. Garand, M. Lafage-Pochitaloff, E. Delabesse, A. Buzyn, et al. CALM-AF10 is a common fusion transcript in T-ALL and is specific to the TCR{gamma}{delta} lineage Blood, August 1, 2003; 102(3): 1000 - 1006. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Klebig, M. D. Wall, M. D. Potter, E. L. Rowe, D. A. Carpenter, and E. M. Rinchik Mutations in the clathrin-assembly gene Picalm are responsible for the hematopoietic and iron metabolism abnormalities in fit1 mice PNAS, July 8, 2003; 100(14): 8360 - 8365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Di Paolo and P. De Camilli Does clathrin pull the fission trigger? PNAS, April 29, 2003; 100(9): 4981 - 4983. [Full Text] [PDF]
|
|
|
|
|
|
C. Kalthoff, J. Alves, C. Urbanke, R. Knorr, and E. J. Ungewickell Unusual Structural Organization of the Endocytic Proteins AP180 and Epsin 1 J. Biol. Chem., March 1, 2002; 277(10): 8209 - 8216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Metzler, V. Legendre-Guillemin, L. Gan, V. Chopra, A. Kwok, P. S. McPherson, and M. R. Hayden HIP1 Functions in Clathrin-mediated Endocytosis through Binding to Clathrin and Adaptor Protein 2 J. Biol. Chem., October 12, 2001; 276(42): 39271 - 39276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Overduin, M. L. Cheever, and T. G. Kutateladze Signaling with Phosphoinositides: Better than Binary Mol. Interv., August 1, 2001; 1(3): 150 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Martina, C. J. Bonangelino, R. C. Aguilar, and J. S. Bonifacino Stonin 2: An Adaptor-like Protein That Interacts with Components of the Endocytic Machinery J. Cell Biol., May 28, 2001; 153(5): 1111 - 1120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. J. Ford, B. M. F. Pearse, M. K. Higgins, Y. Vallis, D. J. Owen, A. Gibson, C. R. Hopkins, P. R. Evans, and H. T. McMahon Simultaneous Binding of PtdIns(4,5)P2 and Clathrin by AP180 in the Nucleation of Clathrin Lattices on Membranes Science, February 9, 2001; 291(5506): 1051 - 1055. [Abstract] [Full Text]
|
|
|
|
|
|
X Zhao, T Greener, H Al-Hasani, S. Cushman, E Eisenberg, and L. Greene Expression of auxilin or AP180 inhibits endocytosis by mislocalizing clathrin: evidence for formation of nascent pits containing AP1 or AP2 but not clathrin J. Cell Sci., January 1, 2001; 114(2): 353 - 365. [Abstract] [PDF]
|
|
|
|
|
|
J. R. Morgan, K. Prasad, W. Hao, G. J. Augustine, and E. M. Lafer A Conserved Clathrin Assembly Motif Essential for Synaptic Vesicle Endocytosis J. Neurosci., December 1, 2000; 20(23): 8667 - 8676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Drake, M. A. Downs, and L. M. Traub Epsin Binds to Clathrin by Associating Directly with the Clathrin-terminal Domain. EVIDENCE FOR COOPERATIVE BINDING THROUGH TWO DISCRETE SITES J. Biol. Chem., February 25, 2000; 275(9): 6479 - 6489. [Abstract] [Full Text] [PDF]
|
|
