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Vol. 10, Issue 8, 2771-2785, August 1999
and
*School of Biological Sciences, University of Manchester,
Manchester M13 9PT, United Kingdom; and Polo kinases execute multiple roles during cell division. The
fission yeast polo related kinase Plo1 is required to assemble the
mitotic spindle, the prophase actin ring that predicts the site for
cytokinesis and for septation after the completion of mitosis (Ohkura
et al., 1995; Bahler et al., 1998). We
show that Plo1 associates with the mitotic but not interphase spindle
pole body (SPB). SPB association of Plo1 is the earliest fission yeast mitotic event recorded to date. SPB association is strong from mitotic
commitment to early anaphase B, after which the Plo1 signal becomes
very weak and finally disappears upon spindle breakdown. SPB
association of Plo1 requires mitosis-promoting factor (MPF) activity,
whereas its disassociation requires the activity of the
anaphase-promoting complex. The stf1.1 mutation bypasses
the usual requirement for the MPF activator Cdc25 (Hudson et
al., 1990). Significantly, Plo1 associates inappropriately with
the interphase SPB of stf1.1 cells. These data are
consistent with the emerging theme from many systems that polo kinases
participate in the regulation of MPF to determine the timing of
commitment to mitosis and may indicate that pole association is a key
aspect of Plo1 function. Plo1 does not associate with the SPB when
septation is inappropriately driven by deregulation of the Spg1 pathway and remains SPB associated if septation occurs in the presence of a
spindle. Thus, neither Plo1 recruitment to nor its departure from the
SPB are required for septation; however, overexpression of
plo1+ activates the Spg1 pathway and causes
transient Cdc7 recruitment to the SPB and multiple rounds of septation.
CRC Cell Cycle
Genetics Group, Department of Anatomy and Physiology, Medical Sciences
Institute, University of Dundee, Dundee DD1 4HN, Scotland
Present address: Institute of Cell and
Molecular Biology, University of Edinburgh, S5.21 Michael Swann
Building, Kings Buildings, Mayfield Road, Edinburgh EH9 3JR, Scotland.
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