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Vol. 10, Issue 9, 2817-2828, September 1999
6
4 Integrin, 1
Integrins, and an E3 Laminin Receptor to Signal Morphogenesis
and -Casein Expression in Mammary Epithelial Cells
and
*Life Sciences Division, Lawrence Berkeley National Laboratory,
Berkeley, California 94720; Contact of cultured mammary epithelial cells with the basement
membrane protein laminin induces multiple responses, including cell
shape changes, growth arrest, and, in the presence of prolactin, transcription of the milk protein Center for Clinical and
Basic Research, Ballerup, Denmark; §Department of Anatomy,
University of British Columbia, Vancouver, British Columbia, Canada;
and Department of Pathology, Robert Woods Johnson
Medical School, Piscataway, New Jersey 08854
-casein. We sought to identify the
specific laminin receptor(s) mediating the multiple cell responses to
laminin. Using assays with clonal mammary epithelial cells, we reveal
distinct functions for the 
64 integrin, 1
integrins, and an E3 laminin receptor. Signals from laminin for
-casein expression were inhibited in the presence of
function-blocking antibodies against both the 6 and 1
integrin subunits and by the laminin E3 fragment. The
6-blocking antibody perturbed signals mediated by the 64
integrin, and the 1-blocking antibody perturbed signals
mediated by another integrin, the subunit(s) of which remains to be determined. Neither 6- nor 1-blocking antibodies perturbed the cell shape changes resulting from cell exposure to
laminin. However, the E3 laminin fragment and heparin both inhibited
cell shape changes induced by laminin, thereby implicating an E3
laminin receptor in this function. These results elucidate the
multiplicity of cell-extracellular matrix interactions required to
integrate cell structure and signaling and ultimately permit normal
cell function.
Corresponding author. E-mail address:
jlmuschler{at}lbl.gov.
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