![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Vol. 10, Issue 9, 2861-2878, September 1999
1-Integrin Function by Epidermal Growth
Factor and Heregulin- Has Distinct Requirements for erbB2 but a
Similar Dependence on Phosphoinositide 3-OH Kinase
and§
*Department of Laboratory Medicine and Pathology,
Integrins and growth factor receptors are important
participants in cellular adhesion and migration. The EGF receptor
(EGFR) family of tyrosine kinases and the Center for Immunology, Cancer Center,
University of Minnesota, Minneapolis, Minnesota 55455
1-integrin
adhesion receptors are of particular interest, given the implication
for their involvement in the initiation and progression of
tumorigenesis. We used adhesion and chemotaxis assays to further
elucidate the relationship between these two families of transmembrane
signaling molecules. Specifically, we examined
integrin-mediated adhesive and migratory characteristics of the
metastatic breast carcinoma cell line MDA-MB-435 in response to
stimulation with growth factors that bind to and activate the EGFR or
erbB3 in these cells. Although ligand engagement of the EGFR stimulated
modest 1-dependent increases in cell adhesion and motility,
heregulin- (HRG) binding to the erbB3 receptor initiated rapid
and potent induction of breast carcinoma cell adhesion and migration
and required dimerization of erbB3 with erbB2. Pharmacologic inhibitors
of phosphoinositide 3-OH kinase (PI 3-K) or transient expression of
dominant negative forms of PI 3-K inhibited both EGF- and
HRG-mediated adhesion and potently blocked HRG- and EGF-induced
cell motility. Our results illustrate the critical role of PI 3-K
activity in signaling pathways initiated by the EGFR or erbB3 to
up-regulate 1-integrin function.
This article has been cited by other articles:
|
|
 |
|
  S. Luangdilok, C. Box, L. Patterson, W. Court, K. Harrington, L. Pitkin, P. Rhys-Evans, P. O-charoenrat, and S. Eccles Syk Tyrosine Kinase Is Linked to Cell Motility and Progression in Squamous Cell Carcinomas of the Head and Neck Cancer Res., August 15, 2007; 67(16): 7907 - 7916. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Belvindrah, S. Hankel, J. Walker, B. L. Patton, and U. Muller {beta}1 Integrins Control the Formation of Cell Chains in the Adult Rostral Migratory Stream J. Neurosci., March 7, 2007; 27(10): 2704 - 2717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Yen, Z. Cao, X. Wu, E. R.Q. Ingalla, C. Baron, L. J.T. Young, J. P. Gregg, R. D. Cardiff, A. D. Borowsky, C. Sweeney, et al. Loss of Nrdp1 Enhances ErbB2/ErbB3-Dependent Breast Tumor Cell Growth Cancer Res., December 1, 2006; 66(23): 11279 - 11286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Xue, F. Liang, R. Mahmood, M. Vuolo, J. Wyckoff, H. Qian, K.-L. Tsai, M. Kim, J. Locker, Z.-Y. Zhang, et al. ErbB3-Dependent Motility and Intravasation in Breast Cancer Metastasis Cancer Res., February 1, 2006; 66(3): 1418 - 1426. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. L. Goel, L. Moro, M. King, N. Teider, M. Centrella, T. L. McCarthy, M. Holgado-Madruga, A. J. Wong, E. Marra, and L. R. Languino {beta}1 Integrins Modulate Cell Adhesion by Regulating Insulin-Like Growth Factor-II Levels in the Microenvironment Cancer Res., January 1, 2006; 66(1): 331 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. V. Lund, M. Popkov, L. Magnenat, and C. F. Barbas III Zinc Finger Transcription Factors Designed for Bispecific Coregulation of ErbB2 and ErbB3 Receptors: Insights into ErbB Receptor Biology Mol. Cell. Biol., October 15, 2005; 25(20): 9082 - 9091. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yang, O. V. Kovalenko, W. Tang, C. Claas, C. S. Stipp, and M. E. Hemler Palmitoylation supports assembly and function of integrin-tetraspanin complexes J. Cell Biol., December 20, 2004; 167(6): 1231 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Goda, A. C. Quale, M. L. Woods, A. Felthauser, and Y. Shimizu Control of TCR-Mediated Activation of {beta}1 Integrins by the ZAP-70 Tyrosine Kinase Interdomain B Region and the Linker for Activation of T Cells Adapter Protein J. Immunol., May 1, 2004; 172(9): 5379 - 5387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li, H. Chen, M.-S. Tang, X. Shi, S. Amin, D. Desai, M. Costa, and C. Huang PI-3K and Akt are mediators of AP-1 induction by 5-MCDE in mouse epidermal Cl41 cells J. Cell Biol., April 12, 2004; 165(1): 77 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-Q. Wang, P. Sun, and A. S. Paller Ganglioside GM3 Blocks the Activation of Epidermal Growth Factor Receptor Induced by Integrin at Specific Tyrosine Sites J. Biol. Chem., December 5, 2003; 278(49): 48770 - 48778. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Bajo, A. V. Schally, M. Krupa, F. Hebert, K. Groot, and K. Szepeshazi Bombesin antagonists inhibit growth of MDA-MB-435 estrogen-independent breast cancers and decrease the expression of the ErbB-2/HER-2 oncoprotein and c-jun and c-fos oncogenes PNAS, March 19, 2002; 99(6): 3836 - 3841. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Schultheis, M. Carapeti-Marootian, A. Hochhaus, A. Weibeta er, J. M. Goldman, and J. V. Melo Overexpression of SOCS-2 in advanced stages of chronic myeloid leukemia: possible inadequacy of a negative feedback mechanism Blood, March 1, 2002; 99(5): 1766 - 1775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Alper, E. S. Bergmann-Leitner, T. A. Bennett, N. F. Hacker, K. Stromberg, and W. G. Stetler-Stevenson Epidermal Growth Factor Receptor Signaling and the Invasive Phenotype of Ovarian Carcinoma Cells J Natl Cancer Inst, September 19, 2001; 93(18): 1375 - 1384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. C. Ottoson, J. T. Pribila, A. S. H. Chan, and Y. Shimizu Cutting Edge: T Cell Migration Regulated by CXCR4 Chemokine Receptor Signaling to ZAP-70 Tyrosine Kinase J. Immunol., August 15, 2001; 167(4): 1857 - 1861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. D. Thor, S. M. Edgerton, S. Liu, D. H. Moore II, and D. J. Kwiatkowski Gelsolin as a Negative Prognostic Factor and Effector of Motility in erbB-2-positive Epidermal Growth Factor Receptor-positive Breast Cancers Clin. Cancer Res., August 1, 2001; 7(8): 2415 - 2424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Woods and Y. Shimizu Signaling networks regulating {beta}1 integrin-mediated adhesion of T lymphocytes to extracellular matrix J. Leukoc. Biol., June 1, 2001; 69(6): 874 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Nakamura, L. Lipfert, G. A. Rodan, and Le T. Duong Convergence of {{alpha}}v{beta}3 Integrin- and Macrophage Colony Stimulating Factor-mediated Signals on Phospholipase C{{gamma}} in Prefusion Osteoclasts J. Cell Biol., January 22, 2001; 152(2): 361 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Hunter, N. Ottoson, N. Boerth, G. A. Koretzky, and Y. Shimizu Cutting Edge: A Novel Function for the SLAP-130/FYB Adapter Protein in {beta}1 Integrin Signaling and T Lymphocyte Migration J. Immunol., February 1, 2000; 164(3): 1143 - 1147. [Abstract] [Full Text] [PDF]
|
|
