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Vol. 11, Issue 1, 325-337, January 2000
Structural Cell Biology Unit, Department of Medical Anatomy, The
Panum Institute, University of Copenhagen, DK-2200 Copenhagen N,
Denmark
Reports on the ultrastructure of cells as well as biochemical data
have, for several years, been indicating a connection between caveolae
and the actin cytoskeleton. Here, using a yeast two-hybrid approach, we
have identified the F-actin cross-linking protein filamin as a ligand
for the caveolae-associated protein caveolin-1. Binding of caveolin-1
to filamin involved the N-terminal region of caveolin-1 and the C
terminus of filamin close to the filamin-dimerization domain. In in
vitro binding assays, recombinant caveolin-1 bound to both nonmuscle
and muscle filamin, indicating that the interaction might not be cell
type specific. With the use of confocal microscopy, colocalization of
caveolin-1 and filamin was observed in elongated patches at the plasma
membrane. Remarkably, when stress fiber formation was induced with
Rho-stimulating Escherichia coli cytotoxic necrotizing
factor 1, the caveolin-1-positive structures became coaligned with
stress fibers, indicating that there was a physical link connecting
them. Immunogold double-labeling electron microscopy confirmed that
caveolin-1-labeled racemose caveolae clusters were positive for
filamin. The actin network, therefore, seems to be directly involved in
the spatial organization of caveolin-1-associated membrane domains.
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 D. D. Doyle, G. Goings, J. Upshaw-Earley, S. K. Ambler, A. Mondul, H. C. Palfrey, and E. Page Dystrophin Associates With Caveolae of Rat Cardiac Myocytes : Relationship to Dystroglycan Circ. Res., September 15, 2000; 87(6): 480 - 488. [Abstract] [Full Text] [PDF]
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M. L. Lu, M. C. Schneider, Y. Zheng, X. Zhang, and J. P. Richie Caveolin-1 Interacts with Androgen Receptor. A POSITIVE MODULATOR OF ANDROGEN RECEPTOR MEDIATED TRANSACTIVATION J. Biol. Chem., April 13, 2001; 276(16): 13442 - 13451. [Abstract] [Full Text] [PDF]
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H. Awata, C. Huang, M. E. Handlogten, and R. T. Miller Interaction of the Calcium-sensing Receptor and Filamin, a Potential Scaffolding Protein J. Biol. Chem., September 7, 2001; 276(37): 34871 - 34879. [Abstract] [Full Text] [PDF]
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G. Hjalm, R. J. MacLeod, O. Kifor, N. Chattopadhyay, and E. M. Brown Filamin-A Binds to the Carboxyl-terminal Tail of the Calcium-sensing Receptor, an Interaction That Participates in CaR-mediated Activation of Mitogen-activated Protein Kinase J. Biol. Chem., September 7, 2001; 276(37): 34880 - 34887. [Abstract] [Full Text] [PDF]
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F. Takada, D. L. V. Woude, H.-Q. Tong, T. G. Thompson, S. C. Watkins, L. M. Kunkel, and A. H. Beggs Myozenin: An alpha -actinin- and gamma -filamin-binding protein of skeletal muscle Z lines PNAS, February 13, 2001; 98(4): 1595 - 1600. [Abstract] [Full Text] [PDF]
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